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The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression
The Polycomb group (PcG) proteins have been implicated in epigenetic transcriptional repression in development, stem cell maintenance and in cancer. The chromodomain protein Polycomb (Pc) is a key member of the PcG. Pc binds to the histone mark, trimethylated histone 3 lysine 27 (H3K27me3), to initi...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5493352/ https://www.ncbi.nlm.nih.gov/pubmed/28665982 http://dx.doi.org/10.1371/journal.pone.0180026 |
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author | Dutta, Pranabananda Li, Willis X. |
author_facet | Dutta, Pranabananda Li, Willis X. |
author_sort | Dutta, Pranabananda |
collection | PubMed |
description | The Polycomb group (PcG) proteins have been implicated in epigenetic transcriptional repression in development, stem cell maintenance and in cancer. The chromodomain protein Polycomb (Pc) is a key member of the PcG. Pc binds to the histone mark, trimethylated histone 3 lysine 27 (H3K27me3), to initiate transcriptional repression. How PcG proteins are recruited to target loci is not fully understood. Here we show that the Drosophila SERTA domain protein Taranis (Tara) is involved in transcriptional regulation of Pc target genes. Embryos lacking Tara exhibit a partial homeotic transformation of cuticular the segments, a phenotype associated with the loss of Pc function. Moreover, Drosophila embryos homozygous for a tara hypomorphic allele also misexpress engrailed, a Pc-regulated gene, and this phenotype is associated with the loss of Pc binding to the cis response element in the engrailed enhancer. In relation to that, Pc recruitment is reduced on the salivary gland polytene chromosomes and specifically at the engrailed locus. These results suggest that Tara might be required for positioning Pc to a subset of its target genes. |
format | Online Article Text |
id | pubmed-5493352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54933522017-07-18 The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression Dutta, Pranabananda Li, Willis X. PLoS One Research Article The Polycomb group (PcG) proteins have been implicated in epigenetic transcriptional repression in development, stem cell maintenance and in cancer. The chromodomain protein Polycomb (Pc) is a key member of the PcG. Pc binds to the histone mark, trimethylated histone 3 lysine 27 (H3K27me3), to initiate transcriptional repression. How PcG proteins are recruited to target loci is not fully understood. Here we show that the Drosophila SERTA domain protein Taranis (Tara) is involved in transcriptional regulation of Pc target genes. Embryos lacking Tara exhibit a partial homeotic transformation of cuticular the segments, a phenotype associated with the loss of Pc function. Moreover, Drosophila embryos homozygous for a tara hypomorphic allele also misexpress engrailed, a Pc-regulated gene, and this phenotype is associated with the loss of Pc binding to the cis response element in the engrailed enhancer. In relation to that, Pc recruitment is reduced on the salivary gland polytene chromosomes and specifically at the engrailed locus. These results suggest that Tara might be required for positioning Pc to a subset of its target genes. Public Library of Science 2017-06-30 /pmc/articles/PMC5493352/ /pubmed/28665982 http://dx.doi.org/10.1371/journal.pone.0180026 Text en © 2017 Dutta, Li http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Dutta, Pranabananda Li, Willis X. The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression |
title | The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression |
title_full | The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression |
title_fullStr | The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression |
title_full_unstemmed | The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression |
title_short | The SERTAD protein Taranis plays a role in Polycomb-mediated gene repression |
title_sort | sertad protein taranis plays a role in polycomb-mediated gene repression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5493352/ https://www.ncbi.nlm.nih.gov/pubmed/28665982 http://dx.doi.org/10.1371/journal.pone.0180026 |
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