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Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective
Surface enhanced Raman scattering (SERS) nanoparticles are an attractive alternative to fluorescent probes for biological labeling because of their photostability and multiplexing capabilities. However, nanoparticle size, shape, and surface properties are known to affect nanoparticle-cell interactio...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5493633/ https://www.ncbi.nlm.nih.gov/pubmed/28667313 http://dx.doi.org/10.1038/s41598-017-04066-0 |
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author | Navas-Moreno, Maria Mehrpouyan, Majid Chernenko, Tatyana Candas, Demet Fan, Ming Li, Jian Jian Yan, Ming Chan, James W. |
author_facet | Navas-Moreno, Maria Mehrpouyan, Majid Chernenko, Tatyana Candas, Demet Fan, Ming Li, Jian Jian Yan, Ming Chan, James W. |
author_sort | Navas-Moreno, Maria |
collection | PubMed |
description | Surface enhanced Raman scattering (SERS) nanoparticles are an attractive alternative to fluorescent probes for biological labeling because of their photostability and multiplexing capabilities. However, nanoparticle size, shape, and surface properties are known to affect nanoparticle-cell interactions. Other issues such as the formation of a protein corona and antibody multivalency interfere with the labeling properties of nanoparticle-antibody conjugates. Hence, it is important to consider these aspects in order to validate such conjugates for live cell imaging applications. Using SERS nanoparticles that target HER2 and CD44 in breast cancer cells, we demonstrate labeling of fixed cells with high specificity that correlates well with fluorescent labels. However, when labeling live cells to monitor surface biomarker expression and dynamics, the nanoparticles are rapidly uptaken by the cells and become compartmentalized into different cellular regions. This behavior is in stark contrast to that of fluorescent antibody conjugates. This study highlights the impact of nanoparticle internalization and trafficking on the ability to use SERS nanoparticle-antibody conjugates to monitor cell dynamics. |
format | Online Article Text |
id | pubmed-5493633 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54936332017-07-05 Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective Navas-Moreno, Maria Mehrpouyan, Majid Chernenko, Tatyana Candas, Demet Fan, Ming Li, Jian Jian Yan, Ming Chan, James W. Sci Rep Article Surface enhanced Raman scattering (SERS) nanoparticles are an attractive alternative to fluorescent probes for biological labeling because of their photostability and multiplexing capabilities. However, nanoparticle size, shape, and surface properties are known to affect nanoparticle-cell interactions. Other issues such as the formation of a protein corona and antibody multivalency interfere with the labeling properties of nanoparticle-antibody conjugates. Hence, it is important to consider these aspects in order to validate such conjugates for live cell imaging applications. Using SERS nanoparticles that target HER2 and CD44 in breast cancer cells, we demonstrate labeling of fixed cells with high specificity that correlates well with fluorescent labels. However, when labeling live cells to monitor surface biomarker expression and dynamics, the nanoparticles are rapidly uptaken by the cells and become compartmentalized into different cellular regions. This behavior is in stark contrast to that of fluorescent antibody conjugates. This study highlights the impact of nanoparticle internalization and trafficking on the ability to use SERS nanoparticle-antibody conjugates to monitor cell dynamics. Nature Publishing Group UK 2017-06-30 /pmc/articles/PMC5493633/ /pubmed/28667313 http://dx.doi.org/10.1038/s41598-017-04066-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Navas-Moreno, Maria Mehrpouyan, Majid Chernenko, Tatyana Candas, Demet Fan, Ming Li, Jian Jian Yan, Ming Chan, James W. Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective |
title | Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective |
title_full | Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective |
title_fullStr | Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective |
title_full_unstemmed | Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective |
title_short | Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective |
title_sort | nanoparticles for live cell microscopy: a surface-enhanced raman scattering perspective |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5493633/ https://www.ncbi.nlm.nih.gov/pubmed/28667313 http://dx.doi.org/10.1038/s41598-017-04066-0 |
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