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Fate predetermination of cardiac myocytes during zebrafish heart regeneration

Adult zebrafish have the remarkable ability to regenerate their heart upon injury, a process that involves limited dedifferentiation and proliferation of spared cardiomyocytes (CMs), and migration of their progeny. During regeneration, proliferating CMs are detected throughout the myocardium, includ...

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Autores principales: Tekeli, Isil, Garcia-Puig, Anna, Notari, Mario, García-Pastor, Cristina, Aujard, Isabelle, Jullien, Ludovic, Raya, Angel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5493782/
https://www.ncbi.nlm.nih.gov/pubmed/28659386
http://dx.doi.org/10.1098/rsob.170116
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author Tekeli, Isil
Garcia-Puig, Anna
Notari, Mario
García-Pastor, Cristina
Aujard, Isabelle
Jullien, Ludovic
Raya, Angel
author_facet Tekeli, Isil
Garcia-Puig, Anna
Notari, Mario
García-Pastor, Cristina
Aujard, Isabelle
Jullien, Ludovic
Raya, Angel
author_sort Tekeli, Isil
collection PubMed
description Adult zebrafish have the remarkable ability to regenerate their heart upon injury, a process that involves limited dedifferentiation and proliferation of spared cardiomyocytes (CMs), and migration of their progeny. During regeneration, proliferating CMs are detected throughout the myocardium, including areas distant to the injury site, but whether all of them are able to contribute to the regenerated tissue remains unknown. Here, we developed a CM-specific, photoinducible genetic labelling system, and show that CMs labelled in embryonic hearts survive and contribute to all three (primordial, trabecular and cortical) layers of the adult zebrafish heart. Next, using this system to investigate the fate of CMs from different parts of the myocardium during regeneration, we show that only CMs immediately adjacent to the injury site contributed to the regenerated tissue. Finally, our results show an extensive predetermination of CM fate during adult heart regeneration, with cells from each myocardial layer giving rise to cells that retain their layer identity in the regenerated myocardium. Overall, our results indicate that adult heart regeneration in the zebrafish is a rather static process governed by short-range signals, in contrast to the highly dynamic plasticity of CM fates that takes place during embryonic heart regeneration.
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spelling pubmed-54937822017-07-05 Fate predetermination of cardiac myocytes during zebrafish heart regeneration Tekeli, Isil Garcia-Puig, Anna Notari, Mario García-Pastor, Cristina Aujard, Isabelle Jullien, Ludovic Raya, Angel Open Biol Research Adult zebrafish have the remarkable ability to regenerate their heart upon injury, a process that involves limited dedifferentiation and proliferation of spared cardiomyocytes (CMs), and migration of their progeny. During regeneration, proliferating CMs are detected throughout the myocardium, including areas distant to the injury site, but whether all of them are able to contribute to the regenerated tissue remains unknown. Here, we developed a CM-specific, photoinducible genetic labelling system, and show that CMs labelled in embryonic hearts survive and contribute to all three (primordial, trabecular and cortical) layers of the adult zebrafish heart. Next, using this system to investigate the fate of CMs from different parts of the myocardium during regeneration, we show that only CMs immediately adjacent to the injury site contributed to the regenerated tissue. Finally, our results show an extensive predetermination of CM fate during adult heart regeneration, with cells from each myocardial layer giving rise to cells that retain their layer identity in the regenerated myocardium. Overall, our results indicate that adult heart regeneration in the zebrafish is a rather static process governed by short-range signals, in contrast to the highly dynamic plasticity of CM fates that takes place during embryonic heart regeneration. The Royal Society 2017-06-28 /pmc/articles/PMC5493782/ /pubmed/28659386 http://dx.doi.org/10.1098/rsob.170116 Text en © 2017 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Research
Tekeli, Isil
Garcia-Puig, Anna
Notari, Mario
García-Pastor, Cristina
Aujard, Isabelle
Jullien, Ludovic
Raya, Angel
Fate predetermination of cardiac myocytes during zebrafish heart regeneration
title Fate predetermination of cardiac myocytes during zebrafish heart regeneration
title_full Fate predetermination of cardiac myocytes during zebrafish heart regeneration
title_fullStr Fate predetermination of cardiac myocytes during zebrafish heart regeneration
title_full_unstemmed Fate predetermination of cardiac myocytes during zebrafish heart regeneration
title_short Fate predetermination of cardiac myocytes during zebrafish heart regeneration
title_sort fate predetermination of cardiac myocytes during zebrafish heart regeneration
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5493782/
https://www.ncbi.nlm.nih.gov/pubmed/28659386
http://dx.doi.org/10.1098/rsob.170116
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