Cargando…
The influence of storage time and temperature on propofol concentrations in canine blood and plasma
Propofol is an intravenous anesthetic commonly used due to its favorable pharmacokinetic and pharmacodynamic profile. There are discrepancies in the literature about the most appropriate sample for determining propofol concentrations. Although plasma has been used for determining propofol concentrat...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494168/ https://www.ncbi.nlm.nih.gov/pubmed/28674652 http://dx.doi.org/10.7717/peerj.3476 |
_version_ | 1783247630725283840 |
---|---|
author | Cox, Sherry Bailey, Joan Okafor, Chika Seddighi, Reza Doherty, Tom |
author_facet | Cox, Sherry Bailey, Joan Okafor, Chika Seddighi, Reza Doherty, Tom |
author_sort | Cox, Sherry |
collection | PubMed |
description | Propofol is an intravenous anesthetic commonly used due to its favorable pharmacokinetic and pharmacodynamic profile. There are discrepancies in the literature about the most appropriate sample for determining propofol concentrations. Although plasma has been used for determining propofol concentrations, whole blood has been the preferred sample. There is also a lack of consistency in the literature on the effect of storage time and temperature on propofol concentrations and this may lead to errors in the design of pharmacokinetic/pharmacodynamics studies. The purpose of this study was to determine the difference in propofol concentrations in whole blood versus plasma and to evaluate the influence of storage time (56 days) and temperature (4 °C, −20 °C, −80 °C) on the stability of propofol concentrations in blood and plasma samples. Results from the study indicate that whole blood and plasma samples containing propofol stored at −80 °C have concentrations as high as or higher than those stored at 4 °C or −20 °C for 56 days; thus, −80 °C is an appropriate temperature for propofol sample storage. Plasma propofol concentrations were consistently higher than whole blood for all three storage temperatures. Consequently, plasma is the most appropriate sample for propofol analysis due to its consistent determinations. |
format | Online Article Text |
id | pubmed-5494168 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-54941682017-07-03 The influence of storage time and temperature on propofol concentrations in canine blood and plasma Cox, Sherry Bailey, Joan Okafor, Chika Seddighi, Reza Doherty, Tom PeerJ Veterinary Medicine Propofol is an intravenous anesthetic commonly used due to its favorable pharmacokinetic and pharmacodynamic profile. There are discrepancies in the literature about the most appropriate sample for determining propofol concentrations. Although plasma has been used for determining propofol concentrations, whole blood has been the preferred sample. There is also a lack of consistency in the literature on the effect of storage time and temperature on propofol concentrations and this may lead to errors in the design of pharmacokinetic/pharmacodynamics studies. The purpose of this study was to determine the difference in propofol concentrations in whole blood versus plasma and to evaluate the influence of storage time (56 days) and temperature (4 °C, −20 °C, −80 °C) on the stability of propofol concentrations in blood and plasma samples. Results from the study indicate that whole blood and plasma samples containing propofol stored at −80 °C have concentrations as high as or higher than those stored at 4 °C or −20 °C for 56 days; thus, −80 °C is an appropriate temperature for propofol sample storage. Plasma propofol concentrations were consistently higher than whole blood for all three storage temperatures. Consequently, plasma is the most appropriate sample for propofol analysis due to its consistent determinations. PeerJ Inc. 2017-06-30 /pmc/articles/PMC5494168/ /pubmed/28674652 http://dx.doi.org/10.7717/peerj.3476 Text en ©2017 Cox et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Veterinary Medicine Cox, Sherry Bailey, Joan Okafor, Chika Seddighi, Reza Doherty, Tom The influence of storage time and temperature on propofol concentrations in canine blood and plasma |
title | The influence of storage time and temperature on propofol concentrations in canine blood and plasma |
title_full | The influence of storage time and temperature on propofol concentrations in canine blood and plasma |
title_fullStr | The influence of storage time and temperature on propofol concentrations in canine blood and plasma |
title_full_unstemmed | The influence of storage time and temperature on propofol concentrations in canine blood and plasma |
title_short | The influence of storage time and temperature on propofol concentrations in canine blood and plasma |
title_sort | influence of storage time and temperature on propofol concentrations in canine blood and plasma |
topic | Veterinary Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494168/ https://www.ncbi.nlm.nih.gov/pubmed/28674652 http://dx.doi.org/10.7717/peerj.3476 |
work_keys_str_mv | AT coxsherry theinfluenceofstoragetimeandtemperatureonpropofolconcentrationsincaninebloodandplasma AT baileyjoan theinfluenceofstoragetimeandtemperatureonpropofolconcentrationsincaninebloodandplasma AT okaforchika theinfluenceofstoragetimeandtemperatureonpropofolconcentrationsincaninebloodandplasma AT seddighireza theinfluenceofstoragetimeandtemperatureonpropofolconcentrationsincaninebloodandplasma AT dohertytom theinfluenceofstoragetimeandtemperatureonpropofolconcentrationsincaninebloodandplasma AT coxsherry influenceofstoragetimeandtemperatureonpropofolconcentrationsincaninebloodandplasma AT baileyjoan influenceofstoragetimeandtemperatureonpropofolconcentrationsincaninebloodandplasma AT okaforchika influenceofstoragetimeandtemperatureonpropofolconcentrationsincaninebloodandplasma AT seddighireza influenceofstoragetimeandtemperatureonpropofolconcentrationsincaninebloodandplasma AT dohertytom influenceofstoragetimeandtemperatureonpropofolconcentrationsincaninebloodandplasma |