Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale
Mitochondria perform central functions in cellular bioenergetics, metabolism, and signaling, and their dysfunction has been linked to numerous diseases. The available studies cover only part of the mitochondrial proteome, and a separation of core mitochondrial proteins from associated fractions has...
Autores principales: | , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494306/ https://www.ncbi.nlm.nih.gov/pubmed/28658629 http://dx.doi.org/10.1016/j.celrep.2017.06.014 |
_version_ | 1783247653062049792 |
---|---|
author | Morgenstern, Marcel Stiller, Sebastian B. Lübbert, Philipp Peikert, Christian D. Dannenmaier, Stefan Drepper, Friedel Weill, Uri Höß, Philipp Feuerstein, Reinhild Gebert, Michael Bohnert, Maria van der Laan, Martin Schuldiner, Maya Schütze, Conny Oeljeklaus, Silke Pfanner, Nikolaus Wiedemann, Nils Warscheid, Bettina |
author_facet | Morgenstern, Marcel Stiller, Sebastian B. Lübbert, Philipp Peikert, Christian D. Dannenmaier, Stefan Drepper, Friedel Weill, Uri Höß, Philipp Feuerstein, Reinhild Gebert, Michael Bohnert, Maria van der Laan, Martin Schuldiner, Maya Schütze, Conny Oeljeklaus, Silke Pfanner, Nikolaus Wiedemann, Nils Warscheid, Bettina |
author_sort | Morgenstern, Marcel |
collection | PubMed |
description | Mitochondria perform central functions in cellular bioenergetics, metabolism, and signaling, and their dysfunction has been linked to numerous diseases. The available studies cover only part of the mitochondrial proteome, and a separation of core mitochondrial proteins from associated fractions has not been achieved. We developed an integrative experimental approach to define the proteome of east mitochondria. We classified > 3,300 proteins of mitochondria and mitochondria-associated fractions and defined 901 high-confidence mitochondrial proteins, expanding the set of mitochondrial proteins by 82. Our analysis includes protein abundance under fermentable and nonfermentable growth, submitochondrial localization, single-protein experiments, and subcellular classification of mitochondria-associated fractions. We identified mitochondrial interactors of respiratory chain supercomplexes, ATP synthase, AAA proteases, the mitochondrial contact site and cristae organizing system (MICOS), and the coenzyme Q biosynthesis cluster, as well as mitochondrial proteins with dual cellular localization. The integrative proteome provides a high-confidence source for the characterization of physiological and pathophysiological functions of mitochondria and their integration into the cellular environment. |
format | Online Article Text |
id | pubmed-5494306 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-54943062017-07-13 Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale Morgenstern, Marcel Stiller, Sebastian B. Lübbert, Philipp Peikert, Christian D. Dannenmaier, Stefan Drepper, Friedel Weill, Uri Höß, Philipp Feuerstein, Reinhild Gebert, Michael Bohnert, Maria van der Laan, Martin Schuldiner, Maya Schütze, Conny Oeljeklaus, Silke Pfanner, Nikolaus Wiedemann, Nils Warscheid, Bettina Cell Rep Resource Mitochondria perform central functions in cellular bioenergetics, metabolism, and signaling, and their dysfunction has been linked to numerous diseases. The available studies cover only part of the mitochondrial proteome, and a separation of core mitochondrial proteins from associated fractions has not been achieved. We developed an integrative experimental approach to define the proteome of east mitochondria. We classified > 3,300 proteins of mitochondria and mitochondria-associated fractions and defined 901 high-confidence mitochondrial proteins, expanding the set of mitochondrial proteins by 82. Our analysis includes protein abundance under fermentable and nonfermentable growth, submitochondrial localization, single-protein experiments, and subcellular classification of mitochondria-associated fractions. We identified mitochondrial interactors of respiratory chain supercomplexes, ATP synthase, AAA proteases, the mitochondrial contact site and cristae organizing system (MICOS), and the coenzyme Q biosynthesis cluster, as well as mitochondrial proteins with dual cellular localization. The integrative proteome provides a high-confidence source for the characterization of physiological and pathophysiological functions of mitochondria and their integration into the cellular environment. Cell Press 2017-06-27 /pmc/articles/PMC5494306/ /pubmed/28658629 http://dx.doi.org/10.1016/j.celrep.2017.06.014 Text en © 2017 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Resource Morgenstern, Marcel Stiller, Sebastian B. Lübbert, Philipp Peikert, Christian D. Dannenmaier, Stefan Drepper, Friedel Weill, Uri Höß, Philipp Feuerstein, Reinhild Gebert, Michael Bohnert, Maria van der Laan, Martin Schuldiner, Maya Schütze, Conny Oeljeklaus, Silke Pfanner, Nikolaus Wiedemann, Nils Warscheid, Bettina Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale |
title | Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale |
title_full | Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale |
title_fullStr | Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale |
title_full_unstemmed | Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale |
title_short | Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale |
title_sort | definition of a high-confidence mitochondrial proteome at quantitative scale |
topic | Resource |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494306/ https://www.ncbi.nlm.nih.gov/pubmed/28658629 http://dx.doi.org/10.1016/j.celrep.2017.06.014 |
work_keys_str_mv | AT morgensternmarcel definitionofahighconfidencemitochondrialproteomeatquantitativescale AT stillersebastianb definitionofahighconfidencemitochondrialproteomeatquantitativescale AT lubbertphilipp definitionofahighconfidencemitochondrialproteomeatquantitativescale AT peikertchristiand definitionofahighconfidencemitochondrialproteomeatquantitativescale AT dannenmaierstefan definitionofahighconfidencemitochondrialproteomeatquantitativescale AT drepperfriedel definitionofahighconfidencemitochondrialproteomeatquantitativescale AT weilluri definitionofahighconfidencemitochondrialproteomeatquantitativescale AT hoßphilipp definitionofahighconfidencemitochondrialproteomeatquantitativescale AT feuersteinreinhild definitionofahighconfidencemitochondrialproteomeatquantitativescale AT gebertmichael definitionofahighconfidencemitochondrialproteomeatquantitativescale AT bohnertmaria definitionofahighconfidencemitochondrialproteomeatquantitativescale AT vanderlaanmartin definitionofahighconfidencemitochondrialproteomeatquantitativescale AT schuldinermaya definitionofahighconfidencemitochondrialproteomeatquantitativescale AT schutzeconny definitionofahighconfidencemitochondrialproteomeatquantitativescale AT oeljeklaussilke definitionofahighconfidencemitochondrialproteomeatquantitativescale AT pfannernikolaus definitionofahighconfidencemitochondrialproteomeatquantitativescale AT wiedemannnils definitionofahighconfidencemitochondrialproteomeatquantitativescale AT warscheidbettina definitionofahighconfidencemitochondrialproteomeatquantitativescale |