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The modulation of two motor behaviors by persistent sodium currents in Xenopus laevis tadpoles

Persistent sodium currents (I(NaP)) are common in neuronal circuitries and have been implicated in several diseases, such as amyotrophic lateral sclerosis (ALS) and epilepsy. However, the role of I(NaP) in the regulation of specific behaviors is still poorly understood. In this study we have charact...

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Autores principales: Svensson, Erik, Jeffreys, Hugo, Li, Wen-Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Physiological Society 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494356/
https://www.ncbi.nlm.nih.gov/pubmed/28331009
http://dx.doi.org/10.1152/jn.00755.2016
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author Svensson, Erik
Jeffreys, Hugo
Li, Wen-Chang
author_facet Svensson, Erik
Jeffreys, Hugo
Li, Wen-Chang
author_sort Svensson, Erik
collection PubMed
description Persistent sodium currents (I(NaP)) are common in neuronal circuitries and have been implicated in several diseases, such as amyotrophic lateral sclerosis (ALS) and epilepsy. However, the role of I(NaP) in the regulation of specific behaviors is still poorly understood. In this study we have characterized I(NaP) and investigated its role in the swimming and struggling behavior of Xenopus tadpoles. I(NaP) was identified in three groups of neurons, namely, sensory Rohon-Beard neurons (RB neurons), descending interneurons (dINs), and non-dINs (neurons rhythmically active in swimming). All groups of neurons expressed I(NaP), but the currents differed in decay time constants, amplitudes, and the membrane potential at which I(NaP) peaked. Low concentrations (1 µM) of the I(NaP) blocker riluzole blocked I(NaP) ~30% and decreased the excitability of the three neuron groups without affecting spike amplitudes or cellular input resistances. Riluzole reduced the number of rebound spikes in dINs and depressed repetitive firing in RB neurons and non-dINs. At the behavior level, riluzole at 1 µM shortened fictive swimming episodes. It also reduced the number of action potentials neurons fired on each struggling cycle. The results show that I(NaP) may play important modulatory roles in motor behaviors. NEW & NOTEWORTHY We have characterized persistent sodium currents in three groups of spinal neurons and their role in shaping spiking activity in the Xenopus tadpole. We then attempted to evaluate the role of persistent sodium currents in regulating tadpole swimming and struggling motor outputs by using low concentrations of the persistent sodium current antagonist riluzole.
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spelling pubmed-54943562017-07-18 The modulation of two motor behaviors by persistent sodium currents in Xenopus laevis tadpoles Svensson, Erik Jeffreys, Hugo Li, Wen-Chang J Neurophysiol Research Article Persistent sodium currents (I(NaP)) are common in neuronal circuitries and have been implicated in several diseases, such as amyotrophic lateral sclerosis (ALS) and epilepsy. However, the role of I(NaP) in the regulation of specific behaviors is still poorly understood. In this study we have characterized I(NaP) and investigated its role in the swimming and struggling behavior of Xenopus tadpoles. I(NaP) was identified in three groups of neurons, namely, sensory Rohon-Beard neurons (RB neurons), descending interneurons (dINs), and non-dINs (neurons rhythmically active in swimming). All groups of neurons expressed I(NaP), but the currents differed in decay time constants, amplitudes, and the membrane potential at which I(NaP) peaked. Low concentrations (1 µM) of the I(NaP) blocker riluzole blocked I(NaP) ~30% and decreased the excitability of the three neuron groups without affecting spike amplitudes or cellular input resistances. Riluzole reduced the number of rebound spikes in dINs and depressed repetitive firing in RB neurons and non-dINs. At the behavior level, riluzole at 1 µM shortened fictive swimming episodes. It also reduced the number of action potentials neurons fired on each struggling cycle. The results show that I(NaP) may play important modulatory roles in motor behaviors. NEW & NOTEWORTHY We have characterized persistent sodium currents in three groups of spinal neurons and their role in shaping spiking activity in the Xenopus tadpole. We then attempted to evaluate the role of persistent sodium currents in regulating tadpole swimming and struggling motor outputs by using low concentrations of the persistent sodium current antagonist riluzole. American Physiological Society 2017-07-01 2017-03-22 /pmc/articles/PMC5494356/ /pubmed/28331009 http://dx.doi.org/10.1152/jn.00755.2016 Text en Copyright © 2017 the American Physiological Society http://creativecommons.org/licenses/by/4.0/deed.en_US Licensed under Creative Commons Attribution CC-BY 4.0 (http://creativecommons.org/licenses/by/4.0/deed.en_US) : © the American Physiological Society.
spellingShingle Research Article
Svensson, Erik
Jeffreys, Hugo
Li, Wen-Chang
The modulation of two motor behaviors by persistent sodium currents in Xenopus laevis tadpoles
title The modulation of two motor behaviors by persistent sodium currents in Xenopus laevis tadpoles
title_full The modulation of two motor behaviors by persistent sodium currents in Xenopus laevis tadpoles
title_fullStr The modulation of two motor behaviors by persistent sodium currents in Xenopus laevis tadpoles
title_full_unstemmed The modulation of two motor behaviors by persistent sodium currents in Xenopus laevis tadpoles
title_short The modulation of two motor behaviors by persistent sodium currents in Xenopus laevis tadpoles
title_sort modulation of two motor behaviors by persistent sodium currents in xenopus laevis tadpoles
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494356/
https://www.ncbi.nlm.nih.gov/pubmed/28331009
http://dx.doi.org/10.1152/jn.00755.2016
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