Comparative study of the methods of extracting mesenchymal stem cells from cryopreserved Wharton’s Jelly

The Wharton’s Jelly (WJ) is an established source of mesenchymal stem cells (MSC). We compared 3 methods of extracting WJ-MSC from cryopreserved tissue and determined that enzymatic digestion of the WJ yielded the most viable MSC, compared to the explant and mechanical digestion methods. The enzymat...

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Detalles Bibliográficos
Autores principales: Boey, Peng Yew Kenny, Lim, Say Liang Daniel, Tang, Kin Fai, Li, Ming Ming, Ekaputra, Andrew Krishna, Chowdhury, Prosanto Kumar, Mukherjee, Rajat Anand Gopal, Teo, Jennifer, Faundo, Arvin C., Chiew, Yoke Fong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of Stem Cells and Regenerative Medicine 2017
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494436/
https://www.ncbi.nlm.nih.gov/pubmed/28684895
Descripción
Sumario:The Wharton’s Jelly (WJ) is an established source of mesenchymal stem cells (MSC). We compared 3 methods of extracting WJ-MSC from cryopreserved tissue and determined that enzymatic digestion of the WJ yielded the most viable MSC, compared to the explant and mechanical digestion methods. The enzymatically-released WJ-MSC conformed to the International Society for Cellular Therapy (ISCT) criteria: displayed plastic-adherence, co-expressed CD73, CD90, CD105 and were negative for hematopoietic lineage cell markers.

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