Comparative study of the methods of extracting mesenchymal stem cells from cryopreserved Wharton’s Jelly

The Wharton’s Jelly (WJ) is an established source of mesenchymal stem cells (MSC). We compared 3 methods of extracting WJ-MSC from cryopreserved tissue and determined that enzymatic digestion of the WJ yielded the most viable MSC, compared to the explant and mechanical digestion methods. The enzymat...

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Detalles Bibliográficos
Autores principales: Boey, Peng Yew Kenny, Lim, Say Liang Daniel, Tang, Kin Fai, Li, Ming Ming, Ekaputra, Andrew Krishna, Chowdhury, Prosanto Kumar, Mukherjee, Rajat Anand Gopal, Teo, Jennifer, Faundo, Arvin C., Chiew, Yoke Fong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of Stem Cells and Regenerative Medicine 2017
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494436/
https://www.ncbi.nlm.nih.gov/pubmed/28684895
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author Boey, Peng Yew Kenny
Lim, Say Liang Daniel
Tang, Kin Fai
Li, Ming Ming
Ekaputra, Andrew Krishna
Chowdhury, Prosanto Kumar
Mukherjee, Rajat Anand Gopal
Teo, Jennifer
Faundo, Arvin C.
Chiew, Yoke Fong
author_facet Boey, Peng Yew Kenny
Lim, Say Liang Daniel
Tang, Kin Fai
Li, Ming Ming
Ekaputra, Andrew Krishna
Chowdhury, Prosanto Kumar
Mukherjee, Rajat Anand Gopal
Teo, Jennifer
Faundo, Arvin C.
Chiew, Yoke Fong
author_sort Boey, Peng Yew Kenny
collection PubMed
description The Wharton’s Jelly (WJ) is an established source of mesenchymal stem cells (MSC). We compared 3 methods of extracting WJ-MSC from cryopreserved tissue and determined that enzymatic digestion of the WJ yielded the most viable MSC, compared to the explant and mechanical digestion methods. The enzymatically-released WJ-MSC conformed to the International Society for Cellular Therapy (ISCT) criteria: displayed plastic-adherence, co-expressed CD73, CD90, CD105 and were negative for hematopoietic lineage cell markers.
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spelling pubmed-54944362017-07-06 Comparative study of the methods of extracting mesenchymal stem cells from cryopreserved Wharton’s Jelly Boey, Peng Yew Kenny Lim, Say Liang Daniel Tang, Kin Fai Li, Ming Ming Ekaputra, Andrew Krishna Chowdhury, Prosanto Kumar Mukherjee, Rajat Anand Gopal Teo, Jennifer Faundo, Arvin C. Chiew, Yoke Fong J Stem Cells Regen Med Brief Communication The Wharton’s Jelly (WJ) is an established source of mesenchymal stem cells (MSC). We compared 3 methods of extracting WJ-MSC from cryopreserved tissue and determined that enzymatic digestion of the WJ yielded the most viable MSC, compared to the explant and mechanical digestion methods. The enzymatically-released WJ-MSC conformed to the International Society for Cellular Therapy (ISCT) criteria: displayed plastic-adherence, co-expressed CD73, CD90, CD105 and were negative for hematopoietic lineage cell markers. Journal of Stem Cells and Regenerative Medicine 2017-05-30 /pmc/articles/PMC5494436/ /pubmed/28684895 Text en Copyright © Journal of Stem Cells and Regenerative Medicine
spellingShingle Brief Communication
Boey, Peng Yew Kenny
Lim, Say Liang Daniel
Tang, Kin Fai
Li, Ming Ming
Ekaputra, Andrew Krishna
Chowdhury, Prosanto Kumar
Mukherjee, Rajat Anand Gopal
Teo, Jennifer
Faundo, Arvin C.
Chiew, Yoke Fong
Comparative study of the methods of extracting mesenchymal stem cells from cryopreserved Wharton’s Jelly
title Comparative study of the methods of extracting mesenchymal stem cells from cryopreserved Wharton’s Jelly
title_full Comparative study of the methods of extracting mesenchymal stem cells from cryopreserved Wharton’s Jelly
title_fullStr Comparative study of the methods of extracting mesenchymal stem cells from cryopreserved Wharton’s Jelly
title_full_unstemmed Comparative study of the methods of extracting mesenchymal stem cells from cryopreserved Wharton’s Jelly
title_short Comparative study of the methods of extracting mesenchymal stem cells from cryopreserved Wharton’s Jelly
title_sort comparative study of the methods of extracting mesenchymal stem cells from cryopreserved wharton’s jelly
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494436/
https://www.ncbi.nlm.nih.gov/pubmed/28684895
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