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Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy
The pathological mechanisms of diabetic retinopathy (DR), a leading cause of blindness in adults with diabetes mellitus, remain incompletely understood. Because microRNAs (miRNAs) represent effective DR therapeutic targets, we identified aberrantly expressed miRNAs associated with cellular dysfuncti...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494571/ https://www.ncbi.nlm.nih.gov/pubmed/28706953 http://dx.doi.org/10.1155/2017/4727942 |
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author | Gong, Qiaoyun Xie, Jia'nan Liu, Yang Li, Ying Su, Guanfang |
author_facet | Gong, Qiaoyun Xie, Jia'nan Liu, Yang Li, Ying Su, Guanfang |
author_sort | Gong, Qiaoyun |
collection | PubMed |
description | The pathological mechanisms of diabetic retinopathy (DR), a leading cause of blindness in adults with diabetes mellitus, remain incompletely understood. Because microRNAs (miRNAs) represent effective DR therapeutic targets, we identified aberrantly expressed miRNAs associated with cellular dysfunction in early DR and detected their potential targets. We exposed human retinal endothelial cells (HRECs) and a cell line of retinal pigment epithelial (RPE) cells to high glucose (25 mmol/L, 1–7 days) to mimic DR progression and used streptozotocin-injected rats (4–8 weeks) for an in vivo diabetes model. HREC/RPE viability decreased after 24 h incubation and diminished further over 6 days, and Hoechst staining revealed hyperglycemia-induced HREC/RPE apoptosis. Although miR-124/-125b expression decreased with DR progression in vitro and in vivo, miR-135b/-199a levels decreased in retinal cells under hyperglycemia exposure, but increased in diabetic retinas. Moreover, miR-145/-146a expression decreased gradually in high-glucose-treated HRECs, but increased in hyperglycemia-exposed RPE cells and in diabetic rats. Our findings suggested that aberrant miRNA expression could be involved in hyperglycemia-induced retinal-cell dysfunction, and the identified miRNAs might vary in different retinal layers, with expression changes associated with DR development. Therefore, miRNA modulation and the targeting of miRNA effects on transcription factors could represent novel and effective DR-treatment strategies. |
format | Online Article Text |
id | pubmed-5494571 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-54945712017-07-13 Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy Gong, Qiaoyun Xie, Jia'nan Liu, Yang Li, Ying Su, Guanfang J Diabetes Res Research Article The pathological mechanisms of diabetic retinopathy (DR), a leading cause of blindness in adults with diabetes mellitus, remain incompletely understood. Because microRNAs (miRNAs) represent effective DR therapeutic targets, we identified aberrantly expressed miRNAs associated with cellular dysfunction in early DR and detected their potential targets. We exposed human retinal endothelial cells (HRECs) and a cell line of retinal pigment epithelial (RPE) cells to high glucose (25 mmol/L, 1–7 days) to mimic DR progression and used streptozotocin-injected rats (4–8 weeks) for an in vivo diabetes model. HREC/RPE viability decreased after 24 h incubation and diminished further over 6 days, and Hoechst staining revealed hyperglycemia-induced HREC/RPE apoptosis. Although miR-124/-125b expression decreased with DR progression in vitro and in vivo, miR-135b/-199a levels decreased in retinal cells under hyperglycemia exposure, but increased in diabetic retinas. Moreover, miR-145/-146a expression decreased gradually in high-glucose-treated HRECs, but increased in hyperglycemia-exposed RPE cells and in diabetic rats. Our findings suggested that aberrant miRNA expression could be involved in hyperglycemia-induced retinal-cell dysfunction, and the identified miRNAs might vary in different retinal layers, with expression changes associated with DR development. Therefore, miRNA modulation and the targeting of miRNA effects on transcription factors could represent novel and effective DR-treatment strategies. Hindawi 2017 2017-06-15 /pmc/articles/PMC5494571/ /pubmed/28706953 http://dx.doi.org/10.1155/2017/4727942 Text en Copyright © 2017 Qiaoyun Gong et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gong, Qiaoyun Xie, Jia'nan Liu, Yang Li, Ying Su, Guanfang Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy |
title | Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy |
title_full | Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy |
title_fullStr | Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy |
title_full_unstemmed | Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy |
title_short | Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy |
title_sort | differentially expressed micrornas in the development of early diabetic retinopathy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494571/ https://www.ncbi.nlm.nih.gov/pubmed/28706953 http://dx.doi.org/10.1155/2017/4727942 |
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