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The developmental proteome of Drosophila melanogaster
Drosophila melanogaster is a widely used genetic model organism in developmental biology. While this model organism has been intensively studied at the RNA level, a comprehensive proteomic study covering the complete life cycle is still missing. Here, we apply label-free quantitative proteomics to e...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5495078/ https://www.ncbi.nlm.nih.gov/pubmed/28381612 http://dx.doi.org/10.1101/gr.213694.116 |
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author | Casas-Vila, Nuria Bluhm, Alina Sayols, Sergi Dinges, Nadja Dejung, Mario Altenhein, Tina Kappei, Dennis Altenhein, Benjamin Roignant, Jean-Yves Butter, Falk |
author_facet | Casas-Vila, Nuria Bluhm, Alina Sayols, Sergi Dinges, Nadja Dejung, Mario Altenhein, Tina Kappei, Dennis Altenhein, Benjamin Roignant, Jean-Yves Butter, Falk |
author_sort | Casas-Vila, Nuria |
collection | PubMed |
description | Drosophila melanogaster is a widely used genetic model organism in developmental biology. While this model organism has been intensively studied at the RNA level, a comprehensive proteomic study covering the complete life cycle is still missing. Here, we apply label-free quantitative proteomics to explore proteome remodeling across Drosophila’s life cycle, resulting in 7952 proteins, and provide a high temporal-resolved embryogenesis proteome of 5458 proteins. Our proteome data enabled us to monitor isoform-specific expression of 34 genes during development, to identify the pseudogene Cyp9f3Ψ as a protein-coding gene, and to obtain evidence of 268 small proteins. Moreover, the comparison with available transcriptomic data uncovered examples of poor correlation between mRNA and protein, underscoring the importance of proteomics to study developmental progression. Data integration of our embryogenesis proteome with tissue-specific data revealed spatial and temporal information for further functional studies of yet uncharacterized proteins. Overall, our high resolution proteomes provide a powerful resource and can be explored in detail in our interactive web interface. |
format | Online Article Text |
id | pubmed-5495078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-54950782018-01-01 The developmental proteome of Drosophila melanogaster Casas-Vila, Nuria Bluhm, Alina Sayols, Sergi Dinges, Nadja Dejung, Mario Altenhein, Tina Kappei, Dennis Altenhein, Benjamin Roignant, Jean-Yves Butter, Falk Genome Res Resource Drosophila melanogaster is a widely used genetic model organism in developmental biology. While this model organism has been intensively studied at the RNA level, a comprehensive proteomic study covering the complete life cycle is still missing. Here, we apply label-free quantitative proteomics to explore proteome remodeling across Drosophila’s life cycle, resulting in 7952 proteins, and provide a high temporal-resolved embryogenesis proteome of 5458 proteins. Our proteome data enabled us to monitor isoform-specific expression of 34 genes during development, to identify the pseudogene Cyp9f3Ψ as a protein-coding gene, and to obtain evidence of 268 small proteins. Moreover, the comparison with available transcriptomic data uncovered examples of poor correlation between mRNA and protein, underscoring the importance of proteomics to study developmental progression. Data integration of our embryogenesis proteome with tissue-specific data revealed spatial and temporal information for further functional studies of yet uncharacterized proteins. Overall, our high resolution proteomes provide a powerful resource and can be explored in detail in our interactive web interface. Cold Spring Harbor Laboratory Press 2017-07 /pmc/articles/PMC5495078/ /pubmed/28381612 http://dx.doi.org/10.1101/gr.213694.116 Text en © 2017 Casas-Vila et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Resource Casas-Vila, Nuria Bluhm, Alina Sayols, Sergi Dinges, Nadja Dejung, Mario Altenhein, Tina Kappei, Dennis Altenhein, Benjamin Roignant, Jean-Yves Butter, Falk The developmental proteome of Drosophila melanogaster |
title | The developmental proteome of Drosophila melanogaster |
title_full | The developmental proteome of Drosophila melanogaster |
title_fullStr | The developmental proteome of Drosophila melanogaster |
title_full_unstemmed | The developmental proteome of Drosophila melanogaster |
title_short | The developmental proteome of Drosophila melanogaster |
title_sort | developmental proteome of drosophila melanogaster |
topic | Resource |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5495078/ https://www.ncbi.nlm.nih.gov/pubmed/28381612 http://dx.doi.org/10.1101/gr.213694.116 |
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