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Modified branched peptides with a histidine-rich tail enhance in vitro gene transfection

Successful gene therapy depends on the development of efficient, non-toxic gene delivery systems. To accomplish this objective, our laboratory has focused on solid-phase synthesized peptide carriers, in which the amino acid sequence can be varied precisely to augment intracellular DNA transport. We...

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Detalles Bibliográficos
Autores principales: Leng, Qixin, Mixson, A. James
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549579/
https://www.ncbi.nlm.nih.gov/pubmed/15731333
http://dx.doi.org/10.1093/nar/gni040
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author Leng, Qixin
Mixson, A. James
author_facet Leng, Qixin
Mixson, A. James
author_sort Leng, Qixin
collection PubMed
description Successful gene therapy depends on the development of efficient, non-toxic gene delivery systems. To accomplish this objective, our laboratory has focused on solid-phase synthesized peptide carriers, in which the amino acid sequence can be varied precisely to augment intracellular DNA transport. We previously determined that linear and branched co-polymers of histidine and lysine in combination with liposomes enhanced the efficiency of gene transfection. In this study, we have modified two branched histidine-lysine (HK) peptides by adding a histidine-rich tail. In a variety of cell lines, this histidine-rich tail markedly improved transfection efficiency, presumably by increasing the buffering capacity of the polymer. One polymer with a histidine-rich tail, H(2)K4bT, compared favorably with the commonly used transfection agents. Together with modification of our transfection protocol, these improved HK peptides alone, without liposomes, are the effective carriers of plasmids into a variety of cells. We anticipate that branched HK peptides will continue to be developed as carriers of nucleic acids for in vitro and in vivo applications.
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spelling pubmed-5495792005-02-26 Modified branched peptides with a histidine-rich tail enhance in vitro gene transfection Leng, Qixin Mixson, A. James Nucleic Acids Res Methods Online Successful gene therapy depends on the development of efficient, non-toxic gene delivery systems. To accomplish this objective, our laboratory has focused on solid-phase synthesized peptide carriers, in which the amino acid sequence can be varied precisely to augment intracellular DNA transport. We previously determined that linear and branched co-polymers of histidine and lysine in combination with liposomes enhanced the efficiency of gene transfection. In this study, we have modified two branched histidine-lysine (HK) peptides by adding a histidine-rich tail. In a variety of cell lines, this histidine-rich tail markedly improved transfection efficiency, presumably by increasing the buffering capacity of the polymer. One polymer with a histidine-rich tail, H(2)K4bT, compared favorably with the commonly used transfection agents. Together with modification of our transfection protocol, these improved HK peptides alone, without liposomes, are the effective carriers of plasmids into a variety of cells. We anticipate that branched HK peptides will continue to be developed as carriers of nucleic acids for in vitro and in vivo applications. Oxford University Press 2005 2005-02-24 /pmc/articles/PMC549579/ /pubmed/15731333 http://dx.doi.org/10.1093/nar/gni040 Text en © The Author 2005. Published by Oxford University Press. All rights reserved
spellingShingle Methods Online
Leng, Qixin
Mixson, A. James
Modified branched peptides with a histidine-rich tail enhance in vitro gene transfection
title Modified branched peptides with a histidine-rich tail enhance in vitro gene transfection
title_full Modified branched peptides with a histidine-rich tail enhance in vitro gene transfection
title_fullStr Modified branched peptides with a histidine-rich tail enhance in vitro gene transfection
title_full_unstemmed Modified branched peptides with a histidine-rich tail enhance in vitro gene transfection
title_short Modified branched peptides with a histidine-rich tail enhance in vitro gene transfection
title_sort modified branched peptides with a histidine-rich tail enhance in vitro gene transfection
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549579/
https://www.ncbi.nlm.nih.gov/pubmed/15731333
http://dx.doi.org/10.1093/nar/gni040
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