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Utility of a novel chromogenic medium as a screening method in the detection of carbapenemase producing Enterobacteriaceae

INTRODUCTION: With the emergence of multidrug resistant Gram-negative bugs, there is an urgent need for rapid detection of these resistant organisms. AIM: This study was performed to assess the utility of a novel chromogenic medium in the detection of carbapenemase producing Enterobacteriaceae from...

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Autores principales: Chaturvedi, Anusha, Banashankari, Gunjiganur Shankarappa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496299/
https://www.ncbi.nlm.nih.gov/pubmed/28706391
http://dx.doi.org/10.4103/0974-2727.208257
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author Chaturvedi, Anusha
Banashankari, Gunjiganur Shankarappa
author_facet Chaturvedi, Anusha
Banashankari, Gunjiganur Shankarappa
author_sort Chaturvedi, Anusha
collection PubMed
description INTRODUCTION: With the emergence of multidrug resistant Gram-negative bugs, there is an urgent need for rapid detection of these resistant organisms. AIM: This study was performed to assess the utility of a novel chromogenic medium in the detection of carbapenemase producing Enterobacteriaceae from various clinical specimens. MATERIALS AND METHODS: A total of 202 isolates of Enterobacteriaceae, which were resistant to meropenem (10 μg) disc by standard disc diffusion method, were analyzed over a period of 6 months. These isolates were subjected to the modified Hodge test (MHT) and inoculated onto the chromogenic medium. Following which the results were analyzed. RESULTS: It was observed that 76.73% of the Enterobacteriaceae gave a positive result on the MHT, 18.31% were negative and 4.95% gave indeterminate result. While 88.11% produced growth on the chromogenic medium, 6.93% yielded no growth and 4.95% gave variable results. Concordance in the results of the two tests was found to be 65.34%. CONCLUSION: Enterobacteriaceae are emerging to be carbapenem resistant and are responsible for the soaring rates of Healthcare Associated Infections (HAIs), especially among critically ill patients. Hence, early detection of the same is important in controlling HAIs.
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spelling pubmed-54962992017-07-13 Utility of a novel chromogenic medium as a screening method in the detection of carbapenemase producing Enterobacteriaceae Chaturvedi, Anusha Banashankari, Gunjiganur Shankarappa J Lab Physicians Original Article INTRODUCTION: With the emergence of multidrug resistant Gram-negative bugs, there is an urgent need for rapid detection of these resistant organisms. AIM: This study was performed to assess the utility of a novel chromogenic medium in the detection of carbapenemase producing Enterobacteriaceae from various clinical specimens. MATERIALS AND METHODS: A total of 202 isolates of Enterobacteriaceae, which were resistant to meropenem (10 μg) disc by standard disc diffusion method, were analyzed over a period of 6 months. These isolates were subjected to the modified Hodge test (MHT) and inoculated onto the chromogenic medium. Following which the results were analyzed. RESULTS: It was observed that 76.73% of the Enterobacteriaceae gave a positive result on the MHT, 18.31% were negative and 4.95% gave indeterminate result. While 88.11% produced growth on the chromogenic medium, 6.93% yielded no growth and 4.95% gave variable results. Concordance in the results of the two tests was found to be 65.34%. CONCLUSION: Enterobacteriaceae are emerging to be carbapenem resistant and are responsible for the soaring rates of Healthcare Associated Infections (HAIs), especially among critically ill patients. Hence, early detection of the same is important in controlling HAIs. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5496299/ /pubmed/28706391 http://dx.doi.org/10.4103/0974-2727.208257 Text en Copyright: © 2017 Journal of Laboratory Physicians http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Chaturvedi, Anusha
Banashankari, Gunjiganur Shankarappa
Utility of a novel chromogenic medium as a screening method in the detection of carbapenemase producing Enterobacteriaceae
title Utility of a novel chromogenic medium as a screening method in the detection of carbapenemase producing Enterobacteriaceae
title_full Utility of a novel chromogenic medium as a screening method in the detection of carbapenemase producing Enterobacteriaceae
title_fullStr Utility of a novel chromogenic medium as a screening method in the detection of carbapenemase producing Enterobacteriaceae
title_full_unstemmed Utility of a novel chromogenic medium as a screening method in the detection of carbapenemase producing Enterobacteriaceae
title_short Utility of a novel chromogenic medium as a screening method in the detection of carbapenemase producing Enterobacteriaceae
title_sort utility of a novel chromogenic medium as a screening method in the detection of carbapenemase producing enterobacteriaceae
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496299/
https://www.ncbi.nlm.nih.gov/pubmed/28706391
http://dx.doi.org/10.4103/0974-2727.208257
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