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Dynamic tracking of pathogenic receptor expression of live cells using pyrenyl glycoanthraquinone-decorated graphene electrodes
Expression of specific transmembrane receptors by cells frequently represents an important signature of diseases, but this dynamic event can hardly be monitored directly with live cells due to the limitation of current biochemical techniques. Here we develop a pyrenyl glycoanthraquinone construct th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496384/ https://www.ncbi.nlm.nih.gov/pubmed/28706649 http://dx.doi.org/10.1039/c4sc03614j |
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author | He, Xiao-Peng Zhu, Bi-Wen Zang, Yi Li, Jia Chen, Guo-Rong Tian, He Long, Yi-Tao |
author_facet | He, Xiao-Peng Zhu, Bi-Wen Zang, Yi Li, Jia Chen, Guo-Rong Tian, He Long, Yi-Tao |
author_sort | He, Xiao-Peng |
collection | PubMed |
description | Expression of specific transmembrane receptors by cells frequently represents an important signature of diseases, but this dynamic event can hardly be monitored directly with live cells due to the limitation of current biochemical techniques. Here we develop a pyrenyl glycoanthraquinone construct that can be firmly immobilized on a graphene-spotted screen printed electrode via strong π-interactions. The inherent current signal produced by the surface-confined glycoquinone can be used to detect selective sugar–protein recognitions with simple electrochemical techniques and portable facilities. Importantly, we demonstrate that the level of pathogenic receptors expressed by different types of live cells can be tracked with the electrode system in a label-free manner, providing a useful tool for the on-demand disease diagnosis as well as basic biochemical studies. |
format | Online Article Text |
id | pubmed-5496384 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-54963842017-07-13 Dynamic tracking of pathogenic receptor expression of live cells using pyrenyl glycoanthraquinone-decorated graphene electrodes He, Xiao-Peng Zhu, Bi-Wen Zang, Yi Li, Jia Chen, Guo-Rong Tian, He Long, Yi-Tao Chem Sci Chemistry Expression of specific transmembrane receptors by cells frequently represents an important signature of diseases, but this dynamic event can hardly be monitored directly with live cells due to the limitation of current biochemical techniques. Here we develop a pyrenyl glycoanthraquinone construct that can be firmly immobilized on a graphene-spotted screen printed electrode via strong π-interactions. The inherent current signal produced by the surface-confined glycoquinone can be used to detect selective sugar–protein recognitions with simple electrochemical techniques and portable facilities. Importantly, we demonstrate that the level of pathogenic receptors expressed by different types of live cells can be tracked with the electrode system in a label-free manner, providing a useful tool for the on-demand disease diagnosis as well as basic biochemical studies. Royal Society of Chemistry 2015-03-01 2015-01-13 /pmc/articles/PMC5496384/ /pubmed/28706649 http://dx.doi.org/10.1039/c4sc03614j Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Chemistry He, Xiao-Peng Zhu, Bi-Wen Zang, Yi Li, Jia Chen, Guo-Rong Tian, He Long, Yi-Tao Dynamic tracking of pathogenic receptor expression of live cells using pyrenyl glycoanthraquinone-decorated graphene electrodes |
title | Dynamic tracking of pathogenic receptor expression of live cells using pyrenyl glycoanthraquinone-decorated graphene electrodes
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title_full | Dynamic tracking of pathogenic receptor expression of live cells using pyrenyl glycoanthraquinone-decorated graphene electrodes
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title_fullStr | Dynamic tracking of pathogenic receptor expression of live cells using pyrenyl glycoanthraquinone-decorated graphene electrodes
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title_full_unstemmed | Dynamic tracking of pathogenic receptor expression of live cells using pyrenyl glycoanthraquinone-decorated graphene electrodes
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title_short | Dynamic tracking of pathogenic receptor expression of live cells using pyrenyl glycoanthraquinone-decorated graphene electrodes
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title_sort | dynamic tracking of pathogenic receptor expression of live cells using pyrenyl glycoanthraquinone-decorated graphene electrodes |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496384/ https://www.ncbi.nlm.nih.gov/pubmed/28706649 http://dx.doi.org/10.1039/c4sc03614j |
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