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A novel method of caenophidian snake sex identification using molecular markers based on two gametologous genes

Sex identification provides important information for ecological and evolutionary studies, as well as benefiting snake conservation management. Traditional methods such as cloacal probing or cloacal popping are counterproductive for sex identification concerning very small species, resulting in diff...

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Autores principales: Laopichienpong, Nararat, Tawichasri, Panupong, Chanhome, Lawan, Phatcharakullawarawat, Rattanin, Singchat, Worapong, Kantachumpoo, Attachai, Muangmai, Narongrit, Suntrarachun, Sunutcha, Matsubara, Kazumi, Peyachoknagul, Surin, Srikulnath, Kornsorn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496543/
https://www.ncbi.nlm.nih.gov/pubmed/28690796
http://dx.doi.org/10.1002/ece3.3057
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author Laopichienpong, Nararat
Tawichasri, Panupong
Chanhome, Lawan
Phatcharakullawarawat, Rattanin
Singchat, Worapong
Kantachumpoo, Attachai
Muangmai, Narongrit
Suntrarachun, Sunutcha
Matsubara, Kazumi
Peyachoknagul, Surin
Srikulnath, Kornsorn
author_facet Laopichienpong, Nararat
Tawichasri, Panupong
Chanhome, Lawan
Phatcharakullawarawat, Rattanin
Singchat, Worapong
Kantachumpoo, Attachai
Muangmai, Narongrit
Suntrarachun, Sunutcha
Matsubara, Kazumi
Peyachoknagul, Surin
Srikulnath, Kornsorn
author_sort Laopichienpong, Nararat
collection PubMed
description Sex identification provides important information for ecological and evolutionary studies, as well as benefiting snake conservation management. Traditional methods such as cloacal probing or cloacal popping are counterproductive for sex identification concerning very small species, resulting in difficulties in the management of their breeding programs. In this study, the nucleotide sequences of gametologous genes (CTNNB1 and WAC genes) were used for the development of molecular sexing markers in caenophidian snakes. Two candidate markers were developed with the two primer sets, and successfully amplified by a single band on the agarose gel in male (ZZ) and two bands, differing in fragment sizes, in female (ZW) of 16 caenophidian snakes for CTNNB1 and 12 caenophidian snakes for WAC. Another candidate marker was developed with the primer set to amplify the specific sequence for CTNNB1W homolog, and the PCR products were successfully obtained in a female‐specific 250‐bp DNA bands. The three candidate PCR sexing markers provide a simple sex identification method based on the amplification of gametologous genes, and they can be used to facilitate effective caenophidian snake conservation and management programs.
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spelling pubmed-54965432017-07-07 A novel method of caenophidian snake sex identification using molecular markers based on two gametologous genes Laopichienpong, Nararat Tawichasri, Panupong Chanhome, Lawan Phatcharakullawarawat, Rattanin Singchat, Worapong Kantachumpoo, Attachai Muangmai, Narongrit Suntrarachun, Sunutcha Matsubara, Kazumi Peyachoknagul, Surin Srikulnath, Kornsorn Ecol Evol Original Research Sex identification provides important information for ecological and evolutionary studies, as well as benefiting snake conservation management. Traditional methods such as cloacal probing or cloacal popping are counterproductive for sex identification concerning very small species, resulting in difficulties in the management of their breeding programs. In this study, the nucleotide sequences of gametologous genes (CTNNB1 and WAC genes) were used for the development of molecular sexing markers in caenophidian snakes. Two candidate markers were developed with the two primer sets, and successfully amplified by a single band on the agarose gel in male (ZZ) and two bands, differing in fragment sizes, in female (ZW) of 16 caenophidian snakes for CTNNB1 and 12 caenophidian snakes for WAC. Another candidate marker was developed with the primer set to amplify the specific sequence for CTNNB1W homolog, and the PCR products were successfully obtained in a female‐specific 250‐bp DNA bands. The three candidate PCR sexing markers provide a simple sex identification method based on the amplification of gametologous genes, and they can be used to facilitate effective caenophidian snake conservation and management programs. John Wiley and Sons Inc. 2017-05-22 /pmc/articles/PMC5496543/ /pubmed/28690796 http://dx.doi.org/10.1002/ece3.3057 Text en © 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Laopichienpong, Nararat
Tawichasri, Panupong
Chanhome, Lawan
Phatcharakullawarawat, Rattanin
Singchat, Worapong
Kantachumpoo, Attachai
Muangmai, Narongrit
Suntrarachun, Sunutcha
Matsubara, Kazumi
Peyachoknagul, Surin
Srikulnath, Kornsorn
A novel method of caenophidian snake sex identification using molecular markers based on two gametologous genes
title A novel method of caenophidian snake sex identification using molecular markers based on two gametologous genes
title_full A novel method of caenophidian snake sex identification using molecular markers based on two gametologous genes
title_fullStr A novel method of caenophidian snake sex identification using molecular markers based on two gametologous genes
title_full_unstemmed A novel method of caenophidian snake sex identification using molecular markers based on two gametologous genes
title_short A novel method of caenophidian snake sex identification using molecular markers based on two gametologous genes
title_sort novel method of caenophidian snake sex identification using molecular markers based on two gametologous genes
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496543/
https://www.ncbi.nlm.nih.gov/pubmed/28690796
http://dx.doi.org/10.1002/ece3.3057
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