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SOX7 expression is critically required in FLK1-expressing cells for vasculogenesis and angiogenesis during mouse embryonic development

The transcriptional program that regulates the differentiation of endothelial precursor cells into a highly organized vascular network is still poorly understood. Here we explore the role of SOX7 during this process, performing a detailed analysis of the vascular defects resulting from either a comp...

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Detalles Bibliográficos
Autores principales: Lilly, Andrew J., Mazan, Andrzej, Scott, Daryl A., Lacaud, Georges, Kouskoff, Valerie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496588/
https://www.ncbi.nlm.nih.gov/pubmed/28577909
http://dx.doi.org/10.1016/j.mod.2017.05.004
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author Lilly, Andrew J.
Mazan, Andrzej
Scott, Daryl A.
Lacaud, Georges
Kouskoff, Valerie
author_facet Lilly, Andrew J.
Mazan, Andrzej
Scott, Daryl A.
Lacaud, Georges
Kouskoff, Valerie
author_sort Lilly, Andrew J.
collection PubMed
description The transcriptional program that regulates the differentiation of endothelial precursor cells into a highly organized vascular network is still poorly understood. Here we explore the role of SOX7 during this process, performing a detailed analysis of the vascular defects resulting from either a complete deficiency in Sox7 expression or from the conditional deletion of Sox7 in FLK1-expressing cells. We analysed the consequence of Sox7 deficiency from E7.5 onward to determine from which stage of development the effect of Sox7 deficiency can be observed. We show that while Sox7 is expressed at the onset of endothelial specification from mesoderm, Sox7 deficiency does not impact the emergence of the first endothelial progenitors. However, by E8.5, clear signs of defective vascular development are already observed with the presence of highly unorganised endothelial cords rather than distinct paired dorsal aorta. By E10.5, both Sox7 complete knockout and FLK1-specific deletion of Sox7 lead to widespread vascular defects. In contrast, while SOX7 is expressed in the earliest specified blood progenitors, the VAV-specific deletion of Sox7 does not affect the hematopoietic system. Together, our data reveal the unique role of SOX7 in vasculogenesis and angiogenesis during embryonic development.
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spelling pubmed-54965882017-08-01 SOX7 expression is critically required in FLK1-expressing cells for vasculogenesis and angiogenesis during mouse embryonic development Lilly, Andrew J. Mazan, Andrzej Scott, Daryl A. Lacaud, Georges Kouskoff, Valerie Mech Dev Article The transcriptional program that regulates the differentiation of endothelial precursor cells into a highly organized vascular network is still poorly understood. Here we explore the role of SOX7 during this process, performing a detailed analysis of the vascular defects resulting from either a complete deficiency in Sox7 expression or from the conditional deletion of Sox7 in FLK1-expressing cells. We analysed the consequence of Sox7 deficiency from E7.5 onward to determine from which stage of development the effect of Sox7 deficiency can be observed. We show that while Sox7 is expressed at the onset of endothelial specification from mesoderm, Sox7 deficiency does not impact the emergence of the first endothelial progenitors. However, by E8.5, clear signs of defective vascular development are already observed with the presence of highly unorganised endothelial cords rather than distinct paired dorsal aorta. By E10.5, both Sox7 complete knockout and FLK1-specific deletion of Sox7 lead to widespread vascular defects. In contrast, while SOX7 is expressed in the earliest specified blood progenitors, the VAV-specific deletion of Sox7 does not affect the hematopoietic system. Together, our data reveal the unique role of SOX7 in vasculogenesis and angiogenesis during embryonic development. Elsevier 2017-08 /pmc/articles/PMC5496588/ /pubmed/28577909 http://dx.doi.org/10.1016/j.mod.2017.05.004 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lilly, Andrew J.
Mazan, Andrzej
Scott, Daryl A.
Lacaud, Georges
Kouskoff, Valerie
SOX7 expression is critically required in FLK1-expressing cells for vasculogenesis and angiogenesis during mouse embryonic development
title SOX7 expression is critically required in FLK1-expressing cells for vasculogenesis and angiogenesis during mouse embryonic development
title_full SOX7 expression is critically required in FLK1-expressing cells for vasculogenesis and angiogenesis during mouse embryonic development
title_fullStr SOX7 expression is critically required in FLK1-expressing cells for vasculogenesis and angiogenesis during mouse embryonic development
title_full_unstemmed SOX7 expression is critically required in FLK1-expressing cells for vasculogenesis and angiogenesis during mouse embryonic development
title_short SOX7 expression is critically required in FLK1-expressing cells for vasculogenesis and angiogenesis during mouse embryonic development
title_sort sox7 expression is critically required in flk1-expressing cells for vasculogenesis and angiogenesis during mouse embryonic development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496588/
https://www.ncbi.nlm.nih.gov/pubmed/28577909
http://dx.doi.org/10.1016/j.mod.2017.05.004
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