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ESCRT-mediated vesicle concatenation in plant endosomes

Ubiquitinated plasma membrane proteins (cargo) are delivered to endosomes and sorted by endosomal sorting complex required for transport (ESCRT) machinery into endosome intralumenal vesicles (ILVs) for degradation. In contrast to the current model that postulates that ILVs form individually from inw...

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Autores principales: Buono, Rafael Andrade, Leier, André, Paez-Valencia, Julio, Pennington, Janice, Goodman, Kaija, Miller, Nathan, Ahlquist, Paul, Marquez-Lago, Tatiana T., Otegui, Marisa S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496621/
https://www.ncbi.nlm.nih.gov/pubmed/28592443
http://dx.doi.org/10.1083/jcb.201612040
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author Buono, Rafael Andrade
Leier, André
Paez-Valencia, Julio
Pennington, Janice
Goodman, Kaija
Miller, Nathan
Ahlquist, Paul
Marquez-Lago, Tatiana T.
Otegui, Marisa S.
author_facet Buono, Rafael Andrade
Leier, André
Paez-Valencia, Julio
Pennington, Janice
Goodman, Kaija
Miller, Nathan
Ahlquist, Paul
Marquez-Lago, Tatiana T.
Otegui, Marisa S.
author_sort Buono, Rafael Andrade
collection PubMed
description Ubiquitinated plasma membrane proteins (cargo) are delivered to endosomes and sorted by endosomal sorting complex required for transport (ESCRT) machinery into endosome intralumenal vesicles (ILVs) for degradation. In contrast to the current model that postulates that ILVs form individually from inward budding of the endosomal limiting membrane, plant ILVs form as networks of concatenated vesicle buds by a novel vesiculation mechanism. We ran computational simulations based on experimentally derived diffusion coefficients of an ESCRT cargo protein and electron tomograms of Arabidopsis thaliana endosomes to measure cargo escape from budding ILVs. We found that 50% of the ESCRT cargo would escape from a single budding profile in 5–20 ms and from three concatenated ILVs in 80–200 ms. These short cargo escape times predict the need for strong diffusion barriers in ILVs. Consistent with a potential role as a diffusion barrier, we find that the ESCRT-III protein SNF7 remains associated with ILVs and is delivered to the vacuole for degradation.
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spelling pubmed-54966212018-01-03 ESCRT-mediated vesicle concatenation in plant endosomes Buono, Rafael Andrade Leier, André Paez-Valencia, Julio Pennington, Janice Goodman, Kaija Miller, Nathan Ahlquist, Paul Marquez-Lago, Tatiana T. Otegui, Marisa S. J Cell Biol Research Articles Ubiquitinated plasma membrane proteins (cargo) are delivered to endosomes and sorted by endosomal sorting complex required for transport (ESCRT) machinery into endosome intralumenal vesicles (ILVs) for degradation. In contrast to the current model that postulates that ILVs form individually from inward budding of the endosomal limiting membrane, plant ILVs form as networks of concatenated vesicle buds by a novel vesiculation mechanism. We ran computational simulations based on experimentally derived diffusion coefficients of an ESCRT cargo protein and electron tomograms of Arabidopsis thaliana endosomes to measure cargo escape from budding ILVs. We found that 50% of the ESCRT cargo would escape from a single budding profile in 5–20 ms and from three concatenated ILVs in 80–200 ms. These short cargo escape times predict the need for strong diffusion barriers in ILVs. Consistent with a potential role as a diffusion barrier, we find that the ESCRT-III protein SNF7 remains associated with ILVs and is delivered to the vacuole for degradation. The Rockefeller University Press 2017-07-03 /pmc/articles/PMC5496621/ /pubmed/28592443 http://dx.doi.org/10.1083/jcb.201612040 Text en © 2017 Buono et al. https://creativecommons.org/licenses/by-nc-sa/4.0/http://www.rupress.org/terms/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Buono, Rafael Andrade
Leier, André
Paez-Valencia, Julio
Pennington, Janice
Goodman, Kaija
Miller, Nathan
Ahlquist, Paul
Marquez-Lago, Tatiana T.
Otegui, Marisa S.
ESCRT-mediated vesicle concatenation in plant endosomes
title ESCRT-mediated vesicle concatenation in plant endosomes
title_full ESCRT-mediated vesicle concatenation in plant endosomes
title_fullStr ESCRT-mediated vesicle concatenation in plant endosomes
title_full_unstemmed ESCRT-mediated vesicle concatenation in plant endosomes
title_short ESCRT-mediated vesicle concatenation in plant endosomes
title_sort escrt-mediated vesicle concatenation in plant endosomes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496621/
https://www.ncbi.nlm.nih.gov/pubmed/28592443
http://dx.doi.org/10.1083/jcb.201612040
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