Cargando…

Ribo-attenuators: novel elements for reliable and modular riboswitch engineering

Riboswitches are structural genetic regulatory elements that directly couple the sensing of small molecules to gene expression. They have considerable potential for applications throughout synthetic biology and bio-manufacturing as they are able to sense a wide range of small molecules and regulate...

Descripción completa

Detalles Bibliográficos
Autores principales: Folliard, Thomas, Mertins, Barbara, Steel, Harrison, Prescott, Thomas P., Newport, Thomas, Jones, Christopher W., Wadhams, George, Bayer, Travis, Armitage, Judith P., Papachristodoulou, Antonis, Rothschild, Lynn J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496857/
https://www.ncbi.nlm.nih.gov/pubmed/28676696
http://dx.doi.org/10.1038/s41598-017-04093-x
_version_ 1783248049537024000
author Folliard, Thomas
Mertins, Barbara
Steel, Harrison
Prescott, Thomas P.
Newport, Thomas
Jones, Christopher W.
Wadhams, George
Bayer, Travis
Armitage, Judith P.
Papachristodoulou, Antonis
Rothschild, Lynn J.
author_facet Folliard, Thomas
Mertins, Barbara
Steel, Harrison
Prescott, Thomas P.
Newport, Thomas
Jones, Christopher W.
Wadhams, George
Bayer, Travis
Armitage, Judith P.
Papachristodoulou, Antonis
Rothschild, Lynn J.
author_sort Folliard, Thomas
collection PubMed
description Riboswitches are structural genetic regulatory elements that directly couple the sensing of small molecules to gene expression. They have considerable potential for applications throughout synthetic biology and bio-manufacturing as they are able to sense a wide range of small molecules and regulate gene expression in response. Despite over a decade of research they have yet to reach this considerable potential as they cannot yet be treated as modular components. This is due to several limitations including sensitivity to changes in genetic context, low tunability, and variability in performance. To overcome the associated difficulties with riboswitches, we have designed and introduced a novel genetic element called a ribo-attenuator in Bacteria. This genetic element allows for predictable tuning, insulation from contextual changes, and a reduction in expression variation. Ribo-attenuators allow riboswitches to be treated as truly modular and tunable components, thus increasing their reliability for a wide range of applications.
format Online
Article
Text
id pubmed-5496857
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-54968572017-07-07 Ribo-attenuators: novel elements for reliable and modular riboswitch engineering Folliard, Thomas Mertins, Barbara Steel, Harrison Prescott, Thomas P. Newport, Thomas Jones, Christopher W. Wadhams, George Bayer, Travis Armitage, Judith P. Papachristodoulou, Antonis Rothschild, Lynn J. Sci Rep Article Riboswitches are structural genetic regulatory elements that directly couple the sensing of small molecules to gene expression. They have considerable potential for applications throughout synthetic biology and bio-manufacturing as they are able to sense a wide range of small molecules and regulate gene expression in response. Despite over a decade of research they have yet to reach this considerable potential as they cannot yet be treated as modular components. This is due to several limitations including sensitivity to changes in genetic context, low tunability, and variability in performance. To overcome the associated difficulties with riboswitches, we have designed and introduced a novel genetic element called a ribo-attenuator in Bacteria. This genetic element allows for predictable tuning, insulation from contextual changes, and a reduction in expression variation. Ribo-attenuators allow riboswitches to be treated as truly modular and tunable components, thus increasing their reliability for a wide range of applications. Nature Publishing Group UK 2017-07-04 /pmc/articles/PMC5496857/ /pubmed/28676696 http://dx.doi.org/10.1038/s41598-017-04093-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Folliard, Thomas
Mertins, Barbara
Steel, Harrison
Prescott, Thomas P.
Newport, Thomas
Jones, Christopher W.
Wadhams, George
Bayer, Travis
Armitage, Judith P.
Papachristodoulou, Antonis
Rothschild, Lynn J.
Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
title Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
title_full Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
title_fullStr Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
title_full_unstemmed Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
title_short Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
title_sort ribo-attenuators: novel elements for reliable and modular riboswitch engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496857/
https://www.ncbi.nlm.nih.gov/pubmed/28676696
http://dx.doi.org/10.1038/s41598-017-04093-x
work_keys_str_mv AT folliardthomas riboattenuatorsnovelelementsforreliableandmodularriboswitchengineering
AT mertinsbarbara riboattenuatorsnovelelementsforreliableandmodularriboswitchengineering
AT steelharrison riboattenuatorsnovelelementsforreliableandmodularriboswitchengineering
AT prescottthomasp riboattenuatorsnovelelementsforreliableandmodularriboswitchengineering
AT newportthomas riboattenuatorsnovelelementsforreliableandmodularriboswitchengineering
AT joneschristopherw riboattenuatorsnovelelementsforreliableandmodularriboswitchengineering
AT wadhamsgeorge riboattenuatorsnovelelementsforreliableandmodularriboswitchengineering
AT bayertravis riboattenuatorsnovelelementsforreliableandmodularriboswitchengineering
AT armitagejudithp riboattenuatorsnovelelementsforreliableandmodularriboswitchengineering
AT papachristodoulouantonis riboattenuatorsnovelelementsforreliableandmodularriboswitchengineering
AT rothschildlynnj riboattenuatorsnovelelementsforreliableandmodularriboswitchengineering