Gateway-Compatible CRISPR-Cas9 Vectors and a Rapid Detection by High-Resolution Melting Curve Analysis

CRISPR-Cas9 system rapidly became an indispensable tool in plant biology to perform targeted mutagenesis. A CRISPR-Cas9-mediated double strand break followed by non-homologous end joining (NHEJ) repair most frequently results in a single base pair deletion or insertions (indels), which is hard to de...

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Detalles Bibliográficos
Autores principales: Denbow, Cynthia J., Lapins, Samantha, Dietz, Nick, Scherer, Raelynn, Nimchuk, Zachary L., Okumoto, Sakiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496963/
https://www.ncbi.nlm.nih.gov/pubmed/28725235
http://dx.doi.org/10.3389/fpls.2017.01171

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