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Mouse models for the study of postnatal cardiac hypertrophy
The main objective of this study was to create a postnatal model for cardiac hypertrophy (CH), in order to explain the mechanisms that are present in childhood cardiac hypertrophy. Five days after implantation, intraperitoneal (IP) isoproterenol (ISO) was injected for 7 days to pregnant female mice....
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5497247/ https://www.ncbi.nlm.nih.gov/pubmed/28785661 http://dx.doi.org/10.1016/j.ijcha.2015.02.005 |
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author | Del Olmo-Turrubiarte, A. Calzada-Torres, A. Díaz-Rosas, G. Palma-Lara, I. Sánchez-Urbina, R. Balderrábano-Saucedo, N.A. González-Márquez, H. Garcia-Alonso, P. Contreras-Ramos, A. |
author_facet | Del Olmo-Turrubiarte, A. Calzada-Torres, A. Díaz-Rosas, G. Palma-Lara, I. Sánchez-Urbina, R. Balderrábano-Saucedo, N.A. González-Márquez, H. Garcia-Alonso, P. Contreras-Ramos, A. |
author_sort | Del Olmo-Turrubiarte, A. |
collection | PubMed |
description | The main objective of this study was to create a postnatal model for cardiac hypertrophy (CH), in order to explain the mechanisms that are present in childhood cardiac hypertrophy. Five days after implantation, intraperitoneal (IP) isoproterenol (ISO) was injected for 7 days to pregnant female mice. The fetuses were obtained at 15, 17 and 19 dpc from both groups, also newborns (NB), neonates (7–15 days) and young adults (6 weeks of age). Histopathological exams were done on the hearts. Immunohistochemistry and western blot demonstrated GATA4 and PCNA protein expression, qPCR real time the mRNA of adrenergic receptors (α-AR and β-AR), alpha and beta myosins (α-MHC, β-MHC) and GATA4. After the administration of ISO, there was no change in the number of offsprings. We observed significant structural changes in the size of the offspring hearts. Morphometric analysis revealed an increase in the size of the left ventricular wall and interventricular septum (IVS). Histopathological analysis demonstrated loss of cellular compaction and presence of left ventricular small fibrous foci after birth. Adrenergic receptors might be responsible for changing a physiological into a pathological hypertrophy. However GATA4 seemed to be the determining factor in the pathology. A new animal model was established for the study of pathologic CH in early postnatal stages. |
format | Online Article Text |
id | pubmed-5497247 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-54972472017-08-07 Mouse models for the study of postnatal cardiac hypertrophy Del Olmo-Turrubiarte, A. Calzada-Torres, A. Díaz-Rosas, G. Palma-Lara, I. Sánchez-Urbina, R. Balderrábano-Saucedo, N.A. González-Márquez, H. Garcia-Alonso, P. Contreras-Ramos, A. Int J Cardiol Heart Vasc Article The main objective of this study was to create a postnatal model for cardiac hypertrophy (CH), in order to explain the mechanisms that are present in childhood cardiac hypertrophy. Five days after implantation, intraperitoneal (IP) isoproterenol (ISO) was injected for 7 days to pregnant female mice. The fetuses were obtained at 15, 17 and 19 dpc from both groups, also newborns (NB), neonates (7–15 days) and young adults (6 weeks of age). Histopathological exams were done on the hearts. Immunohistochemistry and western blot demonstrated GATA4 and PCNA protein expression, qPCR real time the mRNA of adrenergic receptors (α-AR and β-AR), alpha and beta myosins (α-MHC, β-MHC) and GATA4. After the administration of ISO, there was no change in the number of offsprings. We observed significant structural changes in the size of the offspring hearts. Morphometric analysis revealed an increase in the size of the left ventricular wall and interventricular septum (IVS). Histopathological analysis demonstrated loss of cellular compaction and presence of left ventricular small fibrous foci after birth. Adrenergic receptors might be responsible for changing a physiological into a pathological hypertrophy. However GATA4 seemed to be the determining factor in the pathology. A new animal model was established for the study of pathologic CH in early postnatal stages. Elsevier 2015-03-06 /pmc/articles/PMC5497247/ /pubmed/28785661 http://dx.doi.org/10.1016/j.ijcha.2015.02.005 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Del Olmo-Turrubiarte, A. Calzada-Torres, A. Díaz-Rosas, G. Palma-Lara, I. Sánchez-Urbina, R. Balderrábano-Saucedo, N.A. González-Márquez, H. Garcia-Alonso, P. Contreras-Ramos, A. Mouse models for the study of postnatal cardiac hypertrophy |
title | Mouse models for the study of postnatal cardiac hypertrophy |
title_full | Mouse models for the study of postnatal cardiac hypertrophy |
title_fullStr | Mouse models for the study of postnatal cardiac hypertrophy |
title_full_unstemmed | Mouse models for the study of postnatal cardiac hypertrophy |
title_short | Mouse models for the study of postnatal cardiac hypertrophy |
title_sort | mouse models for the study of postnatal cardiac hypertrophy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5497247/ https://www.ncbi.nlm.nih.gov/pubmed/28785661 http://dx.doi.org/10.1016/j.ijcha.2015.02.005 |
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