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Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa

BACKGROUND: Tagetes minuta has a long record of human use for the treatment of stomach and intestinal diseases. Most drugs used for diseases treatment are less efficacious with side effects and this brought the search for new treatment regimens mainly from medicinal plants. METHOD: The essential oil...

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Autores principales: Igwaran, Aboi, Iweriebor, Benson Chucks, Ofuzim Okoh, Sunday, Nwodo, Uchechukwu Uchechukwu, Obi, Larry Chikwelu, Okoh, Anthony Ifeanyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5497343/
https://www.ncbi.nlm.nih.gov/pubmed/28676058
http://dx.doi.org/10.1186/s12906-017-1861-6
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author Igwaran, Aboi
Iweriebor, Benson Chucks
Ofuzim Okoh, Sunday
Nwodo, Uchechukwu Uchechukwu
Obi, Larry Chikwelu
Okoh, Anthony Ifeanyi
author_facet Igwaran, Aboi
Iweriebor, Benson Chucks
Ofuzim Okoh, Sunday
Nwodo, Uchechukwu Uchechukwu
Obi, Larry Chikwelu
Okoh, Anthony Ifeanyi
author_sort Igwaran, Aboi
collection PubMed
description BACKGROUND: Tagetes minuta has a long record of human use for the treatment of stomach and intestinal diseases. Most drugs used for diseases treatment are less efficacious with side effects and this brought the search for new treatment regimens mainly from medicinal plants. METHOD: The essential oil (EO) was extracted by Clevenger’s-type apparatus and its chemical composition, antioxidant and antibacterial properties were determined by GC-MS, spectrophotometric and broth dilution methods respectively. S. uberis, E. cloacae, S. aureus, M. smegmatis, L. ivanovii, Vibrio spp. and E. coli bacteria strains were used as test bacteria. RESULTS: GC-MS analysis revealed 98 compounds in the EO flower of T. minuta and β-Ocimene (14. 40%) was the major chemical constituents. The EO exhibited highest inhibitory effect against DPPH radical, followed by its effect on ABTS, while LP radical showed the least sensitivity with IC(50) values of 2.45 mg/mL, 2.76 mg/mL and 3.23 mg/mL respectively. The EO showed antibacterial activities against all test organisms with MIC value for S. aureus, M. smegatis and S. uberis at 0.125 mg/mL and for L. ivanovii, Vibrio spp., E. cloacae and E. coli at 0.06 mg/mL. The EO showed MBC against E. cloacae and E. coli at 0.06 mg/mL at 0.5 mg/mL for S. uberis and 0.125 mg/mL for Vibrio spp. CONCLUSION: Findings from this study suggest that the EO of T. minuta flower may be a useful candidate in the search for lead constituents for the synthesis of new potent antibacterial and antioxidant agent.
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spelling pubmed-54973432017-07-07 Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa Igwaran, Aboi Iweriebor, Benson Chucks Ofuzim Okoh, Sunday Nwodo, Uchechukwu Uchechukwu Obi, Larry Chikwelu Okoh, Anthony Ifeanyi BMC Complement Altern Med Research Article BACKGROUND: Tagetes minuta has a long record of human use for the treatment of stomach and intestinal diseases. Most drugs used for diseases treatment are less efficacious with side effects and this brought the search for new treatment regimens mainly from medicinal plants. METHOD: The essential oil (EO) was extracted by Clevenger’s-type apparatus and its chemical composition, antioxidant and antibacterial properties were determined by GC-MS, spectrophotometric and broth dilution methods respectively. S. uberis, E. cloacae, S. aureus, M. smegmatis, L. ivanovii, Vibrio spp. and E. coli bacteria strains were used as test bacteria. RESULTS: GC-MS analysis revealed 98 compounds in the EO flower of T. minuta and β-Ocimene (14. 40%) was the major chemical constituents. The EO exhibited highest inhibitory effect against DPPH radical, followed by its effect on ABTS, while LP radical showed the least sensitivity with IC(50) values of 2.45 mg/mL, 2.76 mg/mL and 3.23 mg/mL respectively. The EO showed antibacterial activities against all test organisms with MIC value for S. aureus, M. smegatis and S. uberis at 0.125 mg/mL and for L. ivanovii, Vibrio spp., E. cloacae and E. coli at 0.06 mg/mL. The EO showed MBC against E. cloacae and E. coli at 0.06 mg/mL at 0.5 mg/mL for S. uberis and 0.125 mg/mL for Vibrio spp. CONCLUSION: Findings from this study suggest that the EO of T. minuta flower may be a useful candidate in the search for lead constituents for the synthesis of new potent antibacterial and antioxidant agent. BioMed Central 2017-07-05 /pmc/articles/PMC5497343/ /pubmed/28676058 http://dx.doi.org/10.1186/s12906-017-1861-6 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Igwaran, Aboi
Iweriebor, Benson Chucks
Ofuzim Okoh, Sunday
Nwodo, Uchechukwu Uchechukwu
Obi, Larry Chikwelu
Okoh, Anthony Ifeanyi
Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa
title Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa
title_full Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa
title_fullStr Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa
title_full_unstemmed Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa
title_short Chemical constituents, antibacterial and antioxidant properties of the essential oil flower of Tagetes minuta grown in Cala community Eastern Cape, South Africa
title_sort chemical constituents, antibacterial and antioxidant properties of the essential oil flower of tagetes minuta grown in cala community eastern cape, south africa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5497343/
https://www.ncbi.nlm.nih.gov/pubmed/28676058
http://dx.doi.org/10.1186/s12906-017-1861-6
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