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Deletion of Adseverin in Osteoclasts Affects Cell Structure But Not Bone Metabolism
Adseverin is an actin-severing/capping protein that may contribute to osteoclast differentiation in vitro but its role in bone remodeling of healthy animals is not defined. We analyzed bone and osteoclast structure in adseverin conditional null mice at alveolar and long bone sites. In wild-type and...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5498625/ https://www.ncbi.nlm.nih.gov/pubmed/28389691 http://dx.doi.org/10.1007/s00223-017-0271-6 |
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author | Cao, Yixuan Wang, Yongqiang Sprangers, Sara Picavet, Daisy I. Glogauer, Michael McCulloch, Christopher A. Everts, Vincent |
author_facet | Cao, Yixuan Wang, Yongqiang Sprangers, Sara Picavet, Daisy I. Glogauer, Michael McCulloch, Christopher A. Everts, Vincent |
author_sort | Cao, Yixuan |
collection | PubMed |
description | Adseverin is an actin-severing/capping protein that may contribute to osteoclast differentiation in vitro but its role in bone remodeling of healthy animals is not defined. We analyzed bone and osteoclast structure in adseverin conditional null mice at alveolar and long bone sites. In wild-type and adseverin null mice, as measured by dual-energy X-ray absorptiometry, there were no differences of bone mineral content or bone mineral density, indicating no change of bone metabolism. In tibiae, TRAcP(+) osteoclasts were formed in comparable numbers in adseverin null and wild-type mice. Ultrastructural analysis showed normal and similar abundance of ruffled borders, sealing zones, and mitochondria, and with no difference of osteoclast nuclear numbers. In contrast, analyses of long bone showed that in the absence of adseverin osteoclasts were smaller (120 ± 13 vs. 274 ± 19 µm(2); p < 0.05), as were nuclear size and the surface area of cytoplasm. The nuclei of adseverin null osteoclasts exhibited more heterochromatin (31 ± 3%) than wild-type cells (8 ± 1%), suggesting that adseverin affects cell differentiation. The data indicate that in healthy, developing tissues, adseverin contributes to the regulation of osteoclast structure but not to bone metabolism in vivo. |
format | Online Article Text |
id | pubmed-5498625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-54986252017-07-21 Deletion of Adseverin in Osteoclasts Affects Cell Structure But Not Bone Metabolism Cao, Yixuan Wang, Yongqiang Sprangers, Sara Picavet, Daisy I. Glogauer, Michael McCulloch, Christopher A. Everts, Vincent Calcif Tissue Int Original Research Adseverin is an actin-severing/capping protein that may contribute to osteoclast differentiation in vitro but its role in bone remodeling of healthy animals is not defined. We analyzed bone and osteoclast structure in adseverin conditional null mice at alveolar and long bone sites. In wild-type and adseverin null mice, as measured by dual-energy X-ray absorptiometry, there were no differences of bone mineral content or bone mineral density, indicating no change of bone metabolism. In tibiae, TRAcP(+) osteoclasts were formed in comparable numbers in adseverin null and wild-type mice. Ultrastructural analysis showed normal and similar abundance of ruffled borders, sealing zones, and mitochondria, and with no difference of osteoclast nuclear numbers. In contrast, analyses of long bone showed that in the absence of adseverin osteoclasts were smaller (120 ± 13 vs. 274 ± 19 µm(2); p < 0.05), as were nuclear size and the surface area of cytoplasm. The nuclei of adseverin null osteoclasts exhibited more heterochromatin (31 ± 3%) than wild-type cells (8 ± 1%), suggesting that adseverin affects cell differentiation. The data indicate that in healthy, developing tissues, adseverin contributes to the regulation of osteoclast structure but not to bone metabolism in vivo. Springer US 2017-04-07 2017 /pmc/articles/PMC5498625/ /pubmed/28389691 http://dx.doi.org/10.1007/s00223-017-0271-6 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Research Cao, Yixuan Wang, Yongqiang Sprangers, Sara Picavet, Daisy I. Glogauer, Michael McCulloch, Christopher A. Everts, Vincent Deletion of Adseverin in Osteoclasts Affects Cell Structure But Not Bone Metabolism |
title | Deletion of Adseverin in Osteoclasts Affects Cell Structure But Not Bone Metabolism |
title_full | Deletion of Adseverin in Osteoclasts Affects Cell Structure But Not Bone Metabolism |
title_fullStr | Deletion of Adseverin in Osteoclasts Affects Cell Structure But Not Bone Metabolism |
title_full_unstemmed | Deletion of Adseverin in Osteoclasts Affects Cell Structure But Not Bone Metabolism |
title_short | Deletion of Adseverin in Osteoclasts Affects Cell Structure But Not Bone Metabolism |
title_sort | deletion of adseverin in osteoclasts affects cell structure but not bone metabolism |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5498625/ https://www.ncbi.nlm.nih.gov/pubmed/28389691 http://dx.doi.org/10.1007/s00223-017-0271-6 |
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