Enhancing multiplex genome editing by natural transformation (MuGENT) via inactivation of ssDNA exonucleases

Recently, we described a method for multiplex genome editing by natural transformation (MuGENT). Mutant constructs for MuGENT require large arms of homology (>2000 bp) surrounding each genome edit, which necessitates laborious in vitro DNA splicing. In Vibrio cholerae, we uncover that this requir...

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Detalles Bibliográficos
Autores principales: Dalia, Triana N., Yoon, Soo Hun, Galli, Elisa, Barre, Francois-Xavier, Waters, Christopher M., Dalia, Ankur B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499599/
https://www.ncbi.nlm.nih.gov/pubmed/28575400
http://dx.doi.org/10.1093/nar/gkx496

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499599/
https://www.ncbi.nlm.nih.gov/pubmed/28575400
http://dx.doi.org/10.1093/nar/gkx496

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