Cargando…

RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket

During 30S ribosomal subunit biogenesis, assembly factors are believed to prevent accumulation of misfolded intermediate states of low free energy that slowly convert into mature 30S subunits, namely, kinetically trapped particles. Among the assembly factors, the circularly permuted GTPase, RsgA, pl...

Descripción completa

Detalles Bibliográficos
Autores principales: López-Alonso, Jorge Pedro, Kaminishi, Tatsuya, Kikuchi, Takeshi, Hirata, Yuya, Iturrioz, Idoia, Dhimole, Neha, Schedlbauer, Andreas, Hase, Yoichi, Goto, Simon, Kurita, Daisuke, Muto, Akira, Zhou, Shu, Naoe, Chieko, Mills, Deryck J., Gil-Carton, David, Takemoto, Chie, Himeno, Hyouta, Fucini, Paola, Connell, Sean R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499641/
https://www.ncbi.nlm.nih.gov/pubmed/28482099
http://dx.doi.org/10.1093/nar/gkx324
_version_ 1783248508529147904
author López-Alonso, Jorge Pedro
Kaminishi, Tatsuya
Kikuchi, Takeshi
Hirata, Yuya
Iturrioz, Idoia
Dhimole, Neha
Schedlbauer, Andreas
Hase, Yoichi
Goto, Simon
Kurita, Daisuke
Muto, Akira
Zhou, Shu
Naoe, Chieko
Mills, Deryck J.
Gil-Carton, David
Takemoto, Chie
Himeno, Hyouta
Fucini, Paola
Connell, Sean R.
author_facet López-Alonso, Jorge Pedro
Kaminishi, Tatsuya
Kikuchi, Takeshi
Hirata, Yuya
Iturrioz, Idoia
Dhimole, Neha
Schedlbauer, Andreas
Hase, Yoichi
Goto, Simon
Kurita, Daisuke
Muto, Akira
Zhou, Shu
Naoe, Chieko
Mills, Deryck J.
Gil-Carton, David
Takemoto, Chie
Himeno, Hyouta
Fucini, Paola
Connell, Sean R.
author_sort López-Alonso, Jorge Pedro
collection PubMed
description During 30S ribosomal subunit biogenesis, assembly factors are believed to prevent accumulation of misfolded intermediate states of low free energy that slowly convert into mature 30S subunits, namely, kinetically trapped particles. Among the assembly factors, the circularly permuted GTPase, RsgA, plays a crucial role in the maturation of the 30S decoding center. Here, directed hydroxyl radical probing and single particle cryo-EM are employed to elucidate RsgA΄s mechanism of action. Our results show that RsgA destabilizes the 30S structure, including late binding r-proteins, providing a structural basis for avoiding kinetically trapped assembly intermediates. Moreover, RsgA exploits its distinct GTPase pocket and specific interactions with the 30S to coordinate GTPase activation with the maturation state of the 30S subunit. This coordination validates the architecture of the decoding center and facilitates the timely release of RsgA to control the progression of 30S biogenesis.
format Online
Article
Text
id pubmed-5499641
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-54996412017-07-10 RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket López-Alonso, Jorge Pedro Kaminishi, Tatsuya Kikuchi, Takeshi Hirata, Yuya Iturrioz, Idoia Dhimole, Neha Schedlbauer, Andreas Hase, Yoichi Goto, Simon Kurita, Daisuke Muto, Akira Zhou, Shu Naoe, Chieko Mills, Deryck J. Gil-Carton, David Takemoto, Chie Himeno, Hyouta Fucini, Paola Connell, Sean R. Nucleic Acids Res Structural Biology During 30S ribosomal subunit biogenesis, assembly factors are believed to prevent accumulation of misfolded intermediate states of low free energy that slowly convert into mature 30S subunits, namely, kinetically trapped particles. Among the assembly factors, the circularly permuted GTPase, RsgA, plays a crucial role in the maturation of the 30S decoding center. Here, directed hydroxyl radical probing and single particle cryo-EM are employed to elucidate RsgA΄s mechanism of action. Our results show that RsgA destabilizes the 30S structure, including late binding r-proteins, providing a structural basis for avoiding kinetically trapped assembly intermediates. Moreover, RsgA exploits its distinct GTPase pocket and specific interactions with the 30S to coordinate GTPase activation with the maturation state of the 30S subunit. This coordination validates the architecture of the decoding center and facilitates the timely release of RsgA to control the progression of 30S biogenesis. Oxford University Press 2017-06-20 2017-05-08 /pmc/articles/PMC5499641/ /pubmed/28482099 http://dx.doi.org/10.1093/nar/gkx324 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Structural Biology
López-Alonso, Jorge Pedro
Kaminishi, Tatsuya
Kikuchi, Takeshi
Hirata, Yuya
Iturrioz, Idoia
Dhimole, Neha
Schedlbauer, Andreas
Hase, Yoichi
Goto, Simon
Kurita, Daisuke
Muto, Akira
Zhou, Shu
Naoe, Chieko
Mills, Deryck J.
Gil-Carton, David
Takemoto, Chie
Himeno, Hyouta
Fucini, Paola
Connell, Sean R.
RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket
title RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket
title_full RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket
title_fullStr RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket
title_full_unstemmed RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket
title_short RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket
title_sort rsga couples the maturation state of the 30s ribosomal decoding center to activation of its gtpase pocket
topic Structural Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499641/
https://www.ncbi.nlm.nih.gov/pubmed/28482099
http://dx.doi.org/10.1093/nar/gkx324
work_keys_str_mv AT lopezalonsojorgepedro rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT kaminishitatsuya rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT kikuchitakeshi rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT hiratayuya rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT iturriozidoia rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT dhimoleneha rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT schedlbauerandreas rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT haseyoichi rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT gotosimon rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT kuritadaisuke rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT mutoakira rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT zhoushu rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT naoechieko rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT millsderyckj rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT gilcartondavid rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT takemotochie rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT himenohyouta rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT fucinipaola rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket
AT connellseanr rsgacouplesthematurationstateofthe30sribosomaldecodingcentertoactivationofitsgtpasepocket