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Orientation-dependent FRET system reveals differences in structures and flexibilities of nicked and gapped DNA duplexes

Differences in structures and flexibilities of DNA duplexes play important roles on recognition by DNA-binding proteins. We herein describe a novel method for structural analyses of DNA duplexes by using orientation dependence of Förster resonance energy transfer (FRET). We first analyzed canonical...

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Detalles Bibliográficos
Autores principales:	Kashida, Hiromu, Kurihara, Ayako, Kawai, Hayato, Asanuma, Hiroyuki
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499647/ https://www.ncbi.nlm.nih.gov/pubmed/28369626 http://dx.doi.org/10.1093/nar/gkx200

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