Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery

Realizing the full potential of genome editing requires the development of efficient and broadly applicable methods for delivering programmable nucleases and donor templates for homology-directed repair (HDR). The RNA-guided Cas9 endonuclease can be introduced into cells as a purified protein in com...

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Detalles Bibliográficos
Autores principales: Gaj, Thomas, Staahl, Brett T., Rodrigues, Gonçalo M. C., Limsirichai, Prajit, Ekman, Freja K., Doudna, Jennifer A., Schaffer, David V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499784/
https://www.ncbi.nlm.nih.gov/pubmed/28334779
http://dx.doi.org/10.1093/nar/gkx154

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499784/
https://www.ncbi.nlm.nih.gov/pubmed/28334779
http://dx.doi.org/10.1093/nar/gkx154

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