Cargando…

Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery

Realizing the full potential of genome editing requires the development of efficient and broadly applicable methods for delivering programmable nucleases and donor templates for homology-directed repair (HDR). The RNA-guided Cas9 endonuclease can be introduced into cells as a purified protein in com...

Descripción completa

Detalles Bibliográficos
Autores principales:	Gaj, Thomas, Staahl, Brett T., Rodrigues, Gonçalo M. C., Limsirichai, Prajit, Ekman, Freja K., Doudna, Jennifer A., Schaffer, David V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499784/ https://www.ncbi.nlm.nih.gov/pubmed/28334779 http://dx.doi.org/10.1093/nar/gkx154

Ejemplares similares

In vivo genome editing improves motor function and extends survival in a mouse model of ALS
por: Gaj, Thomas, et al.
Publicado: (2017)

Dynamics and competition of CRISPR–Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editing
por: Fu, Ya-Wen, et al.
Publicado: (2021)

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery
por: Lin, Steven, et al.
Publicado: (2014)

Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair
por: Lee, Kunwoo, et al.
Publicado: (2017)

CRISPR-Cas9-Mediated Genome Editing Increases Lifespan and Improves Motor Deficits in a Huntington’s Disease Mouse Model
por: Ekman, Freja K., et al.
Publicado: (2019)

Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient ‘hit-and-run’ genome editing
por: Lyu, Pin, et al.
Publicado: (2019)

Targeted gene knock-in by CRISPR/Cas ribonucleoproteins in porcine zygotes
por: Park, Ki-Eun, et al.
Publicado: (2017)

Synthetic CRISPR/Cas9 reagents facilitate genome editing and homology directed repair
por: DiNapoli, Sara E, et al.
Publicado: (2020)

Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair
por: He, Xiangjun, et al.
Publicado: (2016)

Transcription-coupled donor DNA expression increases homologous recombination for efficient genome editing
por: Gao, Kaixuan, et al.
Publicado: (2022)

Knocking out consumer concerns and regulator’s rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals
por: Wolter, Felix, et al.
Publicado: (2017)

Genome Editing With TALEN, CRISPR-Cas9 and CRISPR-Cas12a in Combination With AAV6 Homology Donor Restores T Cell Function for XLP
por: Houghton, Benjamin C., et al.
Publicado: (2022)

Computational and molecular tools for scalable rAAV-mediated genome editing
por: Stoimenov, Ivaylo, et al.
Publicado: (2015)

Optimized knock-in of point mutations in zebrafish using CRISPR/Cas9
por: Prykhozhij, Sergey V, et al.
Publicado: (2018)

High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes
por: Paix, Alexandre, et al.
Publicado: (2015)

Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
por: Zhang, Song, et al.
Publicado: (2021)

CRISPR–Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae Nannochloropsis oceanica IMET1
por: Naduthodi, Mihris Ibnu Saleem, et al.
Publicado: (2019)

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
por: Svitashev, Sergei, et al.
Publicado: (2016)

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
por: Farboud, Behnom, et al.
Publicado: (2018)

In Vitro Pre-validation of Gene Editing by CRISPR/Cas9 Ribonucleoprotein
por: Mehravar, Maryam, et al.
Publicado: (2019)

Orthogonal control of expression mean and variance by epigenetic features at different genomic loci
por: Dey, Siddharth S, et al.
Publicado: (2015)

Efficient Generation of Knock-In Zebrafish Models for Inherited Disorders Using CRISPR-Cas9 Ribonucleoprotein Complexes
por: de Vrieze, Erik, et al.
Publicado: (2021)

CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair
por: Katic, Iskra, et al.
Publicado: (2015)

A thermostable Cas9 with increased lifetime in human plasma
por: Harrington, Lucas B., et al.
Publicado: (2017)

CasHRA (Cas9-facilitated Homologous Recombination Assembly) method of constructing megabase-sized DNA
por: Zhou, Jianting, et al.
Publicado: (2016)

CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci
por: Tasan, Ipek, et al.
Publicado: (2018)

Rapid conditional knock-down–knock-in system for mammalian cells
por: Hölzel, Michael, et al.
Publicado: (2007)

Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
por: Oakes, Benjamin L, et al.
Publicado: (2016)

Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination
por: Chen, Changchun, et al.
Publicado: (2013)

Easy quantification of template-directed CRISPR/Cas9 editing
por: Brinkman, Eva K, et al.
Publicado: (2018)

Peptide/Cas9 nanostructures for ribonucleoprotein cell membrane transport and gene edition
por: Lostalé-Seijo, Irene, et al.
Publicado: (2017)

Bacillus subtilis genome editing using ssDNA with short homology regions
por: Wang, Yang, et al.
Publicado: (2012)

Genome-wide activation screens to increase adeno-associated virus production
por: Barnes, Christopher R., et al.
Publicado: (2021)

CRISPR/Cas9-AAV Mediated Knock-in at NRL Locus in Human Embryonic Stem Cells
por: Ge, Xianglian, et al.
Publicado: (2016)

40008 COMBINED CRISPR/CAS9 AND AAV FOR THE GENERATION OF CONDITIONAL ISOGENIC GENE KNOCK-INS
por: Boddu, Prajwal, et al.
Publicado: (2021)

Self-inactivating, all-in-one AAV vectors for precision Cas9 genome editing via homology-directed repair in vivo
por: Ibraheim, Raed, et al.
Publicado: (2021)

A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC–Cas9 or cleavage by Cas9 in living cells
por: St. Martin, Amber, et al.
Publicado: (2018)

CRISPR-CasX is an RNA-dominated enzyme active for human genome editing
por: Liu, Jun-Jie, et al.
Publicado: (2019)

Efficient generation of Knock-in/Knock-out marmoset embryo via CRISPR/Cas9 gene editing
por: Kumita, Wakako, et al.
Publicado: (2019)

CRISPR-Cas9 ribonucleoprotein-mediated co-editing and counterselection in the rice blast fungus
por: Foster, Andrew J., et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_vl9q66iuf7eebs2h7mvs8qb2sg