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The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data

Finding an antibody that works for a specific application can be a difficult task. Hundreds of vendors offer millions of antibodies, but the quality of these products and available validation information varies greatly. In addition, several studies have called into question the reliability of publis...

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Detalles Bibliográficos
Autores principales: Acharya, Poulomi, Quinlan, Anna, Neumeister, Veronique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499787/
https://www.ncbi.nlm.nih.gov/pubmed/28713558
http://dx.doi.org/10.12688/f1000research.11774.1
Descripción
Sumario:Finding an antibody that works for a specific application can be a difficult task. Hundreds of vendors offer millions of antibodies, but the quality of these products and available validation information varies greatly. In addition, several studies have called into question the reliability of published data as the primary metric for assessing antibody quality. We briefly discuss the antibody quality problem and provide best practice guidelines for selecting and validating an antibody, as well as for publishing data generated using antibodies.