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The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data
Finding an antibody that works for a specific application can be a difficult task. Hundreds of vendors offer millions of antibodies, but the quality of these products and available validation information varies greatly. In addition, several studies have called into question the reliability of publis...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
F1000Research
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499787/ https://www.ncbi.nlm.nih.gov/pubmed/28713558 http://dx.doi.org/10.12688/f1000research.11774.1 |
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author | Acharya, Poulomi Quinlan, Anna Neumeister, Veronique |
author_facet | Acharya, Poulomi Quinlan, Anna Neumeister, Veronique |
author_sort | Acharya, Poulomi |
collection | PubMed |
description | Finding an antibody that works for a specific application can be a difficult task. Hundreds of vendors offer millions of antibodies, but the quality of these products and available validation information varies greatly. In addition, several studies have called into question the reliability of published data as the primary metric for assessing antibody quality. We briefly discuss the antibody quality problem and provide best practice guidelines for selecting and validating an antibody, as well as for publishing data generated using antibodies. |
format | Online Article Text |
id | pubmed-5499787 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | F1000Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-54997872017-07-13 The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data Acharya, Poulomi Quinlan, Anna Neumeister, Veronique F1000Res Opinion Article Finding an antibody that works for a specific application can be a difficult task. Hundreds of vendors offer millions of antibodies, but the quality of these products and available validation information varies greatly. In addition, several studies have called into question the reliability of published data as the primary metric for assessing antibody quality. We briefly discuss the antibody quality problem and provide best practice guidelines for selecting and validating an antibody, as well as for publishing data generated using antibodies. F1000Research 2017-06-08 /pmc/articles/PMC5499787/ /pubmed/28713558 http://dx.doi.org/10.12688/f1000research.11774.1 Text en Copyright: © 2017 Acharya P et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Opinion Article Acharya, Poulomi Quinlan, Anna Neumeister, Veronique The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data |
title | The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data |
title_full | The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data |
title_fullStr | The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data |
title_full_unstemmed | The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data |
title_short | The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data |
title_sort | abcs of finding a good antibody: how to find a good antibody, validate it, and publish meaningful data |
topic | Opinion Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499787/ https://www.ncbi.nlm.nih.gov/pubmed/28713558 http://dx.doi.org/10.12688/f1000research.11774.1 |
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