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The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data

Finding an antibody that works for a specific application can be a difficult task. Hundreds of vendors offer millions of antibodies, but the quality of these products and available validation information varies greatly. In addition, several studies have called into question the reliability of publis...

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Detalles Bibliográficos
Autores principales: Acharya, Poulomi, Quinlan, Anna, Neumeister, Veronique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499787/
https://www.ncbi.nlm.nih.gov/pubmed/28713558
http://dx.doi.org/10.12688/f1000research.11774.1
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author Acharya, Poulomi
Quinlan, Anna
Neumeister, Veronique
author_facet Acharya, Poulomi
Quinlan, Anna
Neumeister, Veronique
author_sort Acharya, Poulomi
collection PubMed
description Finding an antibody that works for a specific application can be a difficult task. Hundreds of vendors offer millions of antibodies, but the quality of these products and available validation information varies greatly. In addition, several studies have called into question the reliability of published data as the primary metric for assessing antibody quality. We briefly discuss the antibody quality problem and provide best practice guidelines for selecting and validating an antibody, as well as for publishing data generated using antibodies.
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spelling pubmed-54997872017-07-13 The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data Acharya, Poulomi Quinlan, Anna Neumeister, Veronique F1000Res Opinion Article Finding an antibody that works for a specific application can be a difficult task. Hundreds of vendors offer millions of antibodies, but the quality of these products and available validation information varies greatly. In addition, several studies have called into question the reliability of published data as the primary metric for assessing antibody quality. We briefly discuss the antibody quality problem and provide best practice guidelines for selecting and validating an antibody, as well as for publishing data generated using antibodies. F1000Research 2017-06-08 /pmc/articles/PMC5499787/ /pubmed/28713558 http://dx.doi.org/10.12688/f1000research.11774.1 Text en Copyright: © 2017 Acharya P et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Opinion Article
Acharya, Poulomi
Quinlan, Anna
Neumeister, Veronique
The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data
title The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data
title_full The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data
title_fullStr The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data
title_full_unstemmed The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data
title_short The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data
title_sort abcs of finding a good antibody: how to find a good antibody, validate it, and publish meaningful data
topic Opinion Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499787/
https://www.ncbi.nlm.nih.gov/pubmed/28713558
http://dx.doi.org/10.12688/f1000research.11774.1
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