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WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms

RNA-binding proteins (RBPs) are involved in mRNA splicing, maturation, transport, translation, storage and turnover. Here, we identified ACOT7 mRNA as a novel target of human WIG1. ACOT7 mRNA decay was triggered by the microRNA miR-9 in a WIG1-dependent manner via classic recruitment of Argonaute 2...

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Autores principales: Lee, Hyung Chul, Jung, Seung Hee, Hwang, Hyun Jung, Kang, Donghee, De, Supriyo, Dudekula, Dawood B., Martindale, Jennifer L., Park, Byungkyu, Park, Seung Kuk, Lee, Eun Kyung, Lee, Jeong-Hwa, Jeong, Sunjoo, Han, Kyungsook, Park, Heon Joo, Ko, Young-Gyu, Gorospe, Myriam, Lee, Jae-Seon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499809/
https://www.ncbi.nlm.nih.gov/pubmed/28472401
http://dx.doi.org/10.1093/nar/gkx307
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author Lee, Hyung Chul
Jung, Seung Hee
Hwang, Hyun Jung
Kang, Donghee
De, Supriyo
Dudekula, Dawood B.
Martindale, Jennifer L.
Park, Byungkyu
Park, Seung Kuk
Lee, Eun Kyung
Lee, Jeong-Hwa
Jeong, Sunjoo
Han, Kyungsook
Park, Heon Joo
Ko, Young-Gyu
Gorospe, Myriam
Lee, Jae-Seon
author_facet Lee, Hyung Chul
Jung, Seung Hee
Hwang, Hyun Jung
Kang, Donghee
De, Supriyo
Dudekula, Dawood B.
Martindale, Jennifer L.
Park, Byungkyu
Park, Seung Kuk
Lee, Eun Kyung
Lee, Jeong-Hwa
Jeong, Sunjoo
Han, Kyungsook
Park, Heon Joo
Ko, Young-Gyu
Gorospe, Myriam
Lee, Jae-Seon
author_sort Lee, Hyung Chul
collection PubMed
description RNA-binding proteins (RBPs) are involved in mRNA splicing, maturation, transport, translation, storage and turnover. Here, we identified ACOT7 mRNA as a novel target of human WIG1. ACOT7 mRNA decay was triggered by the microRNA miR-9 in a WIG1-dependent manner via classic recruitment of Argonaute 2 (AGO2). Interestingly, AGO2 was also recruited to ACOT7 mRNA in a WIG1-dependent manner in the absence of miR-9, which indicates an alternative model whereby WIG1 controls AGO2-mediated gene silencing. The WIG1–AGO2 complex attenuated translation initiation via an interaction with translation initiation factor 5B (eIF5B). These results were confirmed using a WIG1 tethering system based on the MS2 bacteriophage coat protein and a reporter construct containing an MS2-binding site, and by immunoprecipitation of WIG1 and detection of WIG1-associated proteins using liquid chromatography-tandem mass spectrometry. We also identified WIG1-binding motifs using photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation analyses. Altogether, our data indicate that WIG1 governs the miRNA-dependent and the miRNA-independent recruitment of AGO2 to lower the stability of and suppress the translation of ACOT7 mRNA.
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spelling pubmed-54998092017-07-12 WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms Lee, Hyung Chul Jung, Seung Hee Hwang, Hyun Jung Kang, Donghee De, Supriyo Dudekula, Dawood B. Martindale, Jennifer L. Park, Byungkyu Park, Seung Kuk Lee, Eun Kyung Lee, Jeong-Hwa Jeong, Sunjoo Han, Kyungsook Park, Heon Joo Ko, Young-Gyu Gorospe, Myriam Lee, Jae-Seon Nucleic Acids Res RNA RNA-binding proteins (RBPs) are involved in mRNA splicing, maturation, transport, translation, storage and turnover. Here, we identified ACOT7 mRNA as a novel target of human WIG1. ACOT7 mRNA decay was triggered by the microRNA miR-9 in a WIG1-dependent manner via classic recruitment of Argonaute 2 (AGO2). Interestingly, AGO2 was also recruited to ACOT7 mRNA in a WIG1-dependent manner in the absence of miR-9, which indicates an alternative model whereby WIG1 controls AGO2-mediated gene silencing. The WIG1–AGO2 complex attenuated translation initiation via an interaction with translation initiation factor 5B (eIF5B). These results were confirmed using a WIG1 tethering system based on the MS2 bacteriophage coat protein and a reporter construct containing an MS2-binding site, and by immunoprecipitation of WIG1 and detection of WIG1-associated proteins using liquid chromatography-tandem mass spectrometry. We also identified WIG1-binding motifs using photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation analyses. Altogether, our data indicate that WIG1 governs the miRNA-dependent and the miRNA-independent recruitment of AGO2 to lower the stability of and suppress the translation of ACOT7 mRNA. Oxford University Press 2017-06-20 2017-05-03 /pmc/articles/PMC5499809/ /pubmed/28472401 http://dx.doi.org/10.1093/nar/gkx307 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle RNA
Lee, Hyung Chul
Jung, Seung Hee
Hwang, Hyun Jung
Kang, Donghee
De, Supriyo
Dudekula, Dawood B.
Martindale, Jennifer L.
Park, Byungkyu
Park, Seung Kuk
Lee, Eun Kyung
Lee, Jeong-Hwa
Jeong, Sunjoo
Han, Kyungsook
Park, Heon Joo
Ko, Young-Gyu
Gorospe, Myriam
Lee, Jae-Seon
WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms
title WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms
title_full WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms
title_fullStr WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms
title_full_unstemmed WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms
title_short WIG1 is crucial for AGO2-mediated ACOT7 mRNA silencing via miRNA-dependent and -independent mechanisms
title_sort wig1 is crucial for ago2-mediated acot7 mrna silencing via mirna-dependent and -independent mechanisms
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499809/
https://www.ncbi.nlm.nih.gov/pubmed/28472401
http://dx.doi.org/10.1093/nar/gkx307
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