Flexible CRISPR library construction using parallel oligonucleotide retrieval

CRISPR/Cas9-based gene knockout libraries have emerged as a powerful tool for functional screens. We present here a set of pre-designed human and mouse sgRNA sequences that are optimized for both high on-target potency and low off-target effect. To maximize the chance of target gene inactivation, sg...

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Detalles Bibliográficos
Autores principales: Read, Abigail, Gao, Shaojian, Batchelor, Eric, Luo, Ji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499874/
https://www.ncbi.nlm.nih.gov/pubmed/28334828
http://dx.doi.org/10.1093/nar/gkx181

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499874/
https://www.ncbi.nlm.nih.gov/pubmed/28334828
http://dx.doi.org/10.1093/nar/gkx181

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