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Circulating fibrocytes are involved in inflammation and leukocyte trafficking in neonates with necrotizing enterocolitis

Fibrocytes, ahematopoietic stem cell source of fibroblasts/myofibroblasts, were previously implicated to infiltrate into the intestinal and enhance inflammation. The aims of the present study were to elucidate the role of fibrocytes in necrotizing enterocolitis (NEC) pathogenesis and to explore the...

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Autores principales: Liu, Ye, Qingjuan, Shang, Gao, Zongwei, Deng, Chun, Wang, Yan, Guo, Chunbao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500098/
https://www.ncbi.nlm.nih.gov/pubmed/28658176
http://dx.doi.org/10.1097/MD.0000000000007400
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author Liu, Ye
Qingjuan, Shang
Gao, Zongwei
Deng, Chun
Wang, Yan
Guo, Chunbao
author_facet Liu, Ye
Qingjuan, Shang
Gao, Zongwei
Deng, Chun
Wang, Yan
Guo, Chunbao
author_sort Liu, Ye
collection PubMed
description Fibrocytes, ahematopoietic stem cell source of fibroblasts/myofibroblasts, were previously implicated to infiltrate into the intestinal and enhance inflammation. The aims of the present study were to elucidate the role of fibrocytes in necrotizing enterocolitis (NEC) pathogenesis and to explore the mechanisms by which fibrocytes contributed to the inflammatory responses. We investigated circulating and intestinal local fibrocytes from 32 patients with NEC, 8 patients with noninflammatory conditions of the gastrointestinal tract and 12 normal subjects. Significantly higher numbers of circulating fibrocytes were found in the peripheral blood from NEC patients than the controls (P < .01). Numerous fibrocytes were found infiltrating the NEC intestinal mucous membranes. The percentage of fibrocytes to total leukocytes in the NEC inflammatory lesions was significantly increased compared with the percentage in the noninflammatory gastrointestinal tract. The fibrocyte attractant chemokine C-X-C motif chemokine ligand 12 (CXCL12) was significantly increased in the plasma and was detectable in 80% of the peritoneal lavage fluid from NEC patients but not the controls. Furthermore, chemokine expression was increased in fibrocytes infiltrating and trafficking to leukocyte sites. In culture, lipopolysaccharide (LPS) induced a significant increase in the expression of the Toll-like receptor (TLR4) signal, with the upregulation of p38 in both the isolated fibrocytes and macrophages. Similarly, interleukin (IL)-1β induced increased the upregulation of the IL-6, tumor necrosis factor (TNF)-α, and intercellular cell adhesion molecule-1 mRNAs but downregulated ColI in fibrocytes isolated from NEC patients compared with the controls. These findings indicate that circulating fibrocytes are increased in NEC patients and may be recruited to the inflammatory intestinal track, most likely through the CXCR4/CXCL12 axis. These cells may contribute to intestinal inflammation through TLR4 signaling by producing the TNF-α and IL-6 cytokines.
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spelling pubmed-55000982017-07-17 Circulating fibrocytes are involved in inflammation and leukocyte trafficking in neonates with necrotizing enterocolitis Liu, Ye Qingjuan, Shang Gao, Zongwei Deng, Chun Wang, Yan Guo, Chunbao Medicine (Baltimore) 3600 Fibrocytes, ahematopoietic stem cell source of fibroblasts/myofibroblasts, were previously implicated to infiltrate into the intestinal and enhance inflammation. The aims of the present study were to elucidate the role of fibrocytes in necrotizing enterocolitis (NEC) pathogenesis and to explore the mechanisms by which fibrocytes contributed to the inflammatory responses. We investigated circulating and intestinal local fibrocytes from 32 patients with NEC, 8 patients with noninflammatory conditions of the gastrointestinal tract and 12 normal subjects. Significantly higher numbers of circulating fibrocytes were found in the peripheral blood from NEC patients than the controls (P < .01). Numerous fibrocytes were found infiltrating the NEC intestinal mucous membranes. The percentage of fibrocytes to total leukocytes in the NEC inflammatory lesions was significantly increased compared with the percentage in the noninflammatory gastrointestinal tract. The fibrocyte attractant chemokine C-X-C motif chemokine ligand 12 (CXCL12) was significantly increased in the plasma and was detectable in 80% of the peritoneal lavage fluid from NEC patients but not the controls. Furthermore, chemokine expression was increased in fibrocytes infiltrating and trafficking to leukocyte sites. In culture, lipopolysaccharide (LPS) induced a significant increase in the expression of the Toll-like receptor (TLR4) signal, with the upregulation of p38 in both the isolated fibrocytes and macrophages. Similarly, interleukin (IL)-1β induced increased the upregulation of the IL-6, tumor necrosis factor (TNF)-α, and intercellular cell adhesion molecule-1 mRNAs but downregulated ColI in fibrocytes isolated from NEC patients compared with the controls. These findings indicate that circulating fibrocytes are increased in NEC patients and may be recruited to the inflammatory intestinal track, most likely through the CXCR4/CXCL12 axis. These cells may contribute to intestinal inflammation through TLR4 signaling by producing the TNF-α and IL-6 cytokines. Wolters Kluwer Health 2017-06-30 /pmc/articles/PMC5500098/ /pubmed/28658176 http://dx.doi.org/10.1097/MD.0000000000007400 Text en Copyright © 2017 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by-nc/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc/4.0
spellingShingle 3600
Liu, Ye
Qingjuan, Shang
Gao, Zongwei
Deng, Chun
Wang, Yan
Guo, Chunbao
Circulating fibrocytes are involved in inflammation and leukocyte trafficking in neonates with necrotizing enterocolitis
title Circulating fibrocytes are involved in inflammation and leukocyte trafficking in neonates with necrotizing enterocolitis
title_full Circulating fibrocytes are involved in inflammation and leukocyte trafficking in neonates with necrotizing enterocolitis
title_fullStr Circulating fibrocytes are involved in inflammation and leukocyte trafficking in neonates with necrotizing enterocolitis
title_full_unstemmed Circulating fibrocytes are involved in inflammation and leukocyte trafficking in neonates with necrotizing enterocolitis
title_short Circulating fibrocytes are involved in inflammation and leukocyte trafficking in neonates with necrotizing enterocolitis
title_sort circulating fibrocytes are involved in inflammation and leukocyte trafficking in neonates with necrotizing enterocolitis
topic 3600
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500098/
https://www.ncbi.nlm.nih.gov/pubmed/28658176
http://dx.doi.org/10.1097/MD.0000000000007400
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