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Pervasive Behavioral Effects of MicroRNA Regulation in Drosophila
The effects of microRNA (miRNA) regulation on the genetic programs underlying behavior remain largely unexplored. Despite this, recent work in Drosophila shows that mutation of a single miRNA locus (miR-iab4/iab8) affects the capacity of the larva to correct its orientation if turned upside down (se...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500149/ https://www.ncbi.nlm.nih.gov/pubmed/28468905 http://dx.doi.org/10.1534/genetics.116.195776 |
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author | Picao-Osorio, Joao Lago-Baldaia, Ines Patraquim, Pedro Alonso, Claudio R. |
author_facet | Picao-Osorio, Joao Lago-Baldaia, Ines Patraquim, Pedro Alonso, Claudio R. |
author_sort | Picao-Osorio, Joao |
collection | PubMed |
description | The effects of microRNA (miRNA) regulation on the genetic programs underlying behavior remain largely unexplored. Despite this, recent work in Drosophila shows that mutation of a single miRNA locus (miR-iab4/iab8) affects the capacity of the larva to correct its orientation if turned upside down (self-righting, SR), suggesting that other miRNAs might also be involved in behavioral control. Here we explore this possibility, studying early larval SR behavior in a collection of 81 Drosophila miRNA mutants covering almost the entire miRNA complement of the late embryo. Unexpectedly, we observe that >40% of all miRNAs tested significantly affect SR time, revealing pervasive behavioral effects of miRNA regulation in the early larva. Detailed analyses of those miRNAs affecting SR behavior (SR-miRNAs) show that individual miRNAs can affect movement in different ways, suggesting that specific molecular and cellular elements are affected by individual miRNA mutations. Furthermore, gene expression analysis shows that the Hox gene Abdominal-B (Abd-B) represents one of the targets deregulated by several SR-miRNAs. Our work thus reveals pervasive effects of miRNA regulation on a complex innate behavior in Drosophila and suggests that miRNAs may be core components of the genetic programs underlying behavioral control in other animals too. |
format | Online Article Text |
id | pubmed-5500149 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-55001492017-07-07 Pervasive Behavioral Effects of MicroRNA Regulation in Drosophila Picao-Osorio, Joao Lago-Baldaia, Ines Patraquim, Pedro Alonso, Claudio R. Genetics Investigations The effects of microRNA (miRNA) regulation on the genetic programs underlying behavior remain largely unexplored. Despite this, recent work in Drosophila shows that mutation of a single miRNA locus (miR-iab4/iab8) affects the capacity of the larva to correct its orientation if turned upside down (self-righting, SR), suggesting that other miRNAs might also be involved in behavioral control. Here we explore this possibility, studying early larval SR behavior in a collection of 81 Drosophila miRNA mutants covering almost the entire miRNA complement of the late embryo. Unexpectedly, we observe that >40% of all miRNAs tested significantly affect SR time, revealing pervasive behavioral effects of miRNA regulation in the early larva. Detailed analyses of those miRNAs affecting SR behavior (SR-miRNAs) show that individual miRNAs can affect movement in different ways, suggesting that specific molecular and cellular elements are affected by individual miRNA mutations. Furthermore, gene expression analysis shows that the Hox gene Abdominal-B (Abd-B) represents one of the targets deregulated by several SR-miRNAs. Our work thus reveals pervasive effects of miRNA regulation on a complex innate behavior in Drosophila and suggests that miRNAs may be core components of the genetic programs underlying behavioral control in other animals too. Genetics Society of America 2017-07 2017-05-02 /pmc/articles/PMC5500149/ /pubmed/28468905 http://dx.doi.org/10.1534/genetics.116.195776 Text en Copyright © 2017 Picao-Osorio et al. Available freely online through the author-supported open access option. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Investigations Picao-Osorio, Joao Lago-Baldaia, Ines Patraquim, Pedro Alonso, Claudio R. Pervasive Behavioral Effects of MicroRNA Regulation in Drosophila |
title | Pervasive Behavioral Effects of MicroRNA Regulation in Drosophila |
title_full | Pervasive Behavioral Effects of MicroRNA Regulation in Drosophila |
title_fullStr | Pervasive Behavioral Effects of MicroRNA Regulation in Drosophila |
title_full_unstemmed | Pervasive Behavioral Effects of MicroRNA Regulation in Drosophila |
title_short | Pervasive Behavioral Effects of MicroRNA Regulation in Drosophila |
title_sort | pervasive behavioral effects of microrna regulation in drosophila |
topic | Investigations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500149/ https://www.ncbi.nlm.nih.gov/pubmed/28468905 http://dx.doi.org/10.1534/genetics.116.195776 |
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