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Plasma Membrane is Compartmentalized by a Self-Similar Cortical Actin Meshwork
A broad range of membrane proteins display anomalous diffusion on the cell surface. Different methods provide evidence for obstructed subdiffusion and diffusion on a fractal space, but the underlying structure inducing anomalous diffusion has never been visualized because of experimental challenges....
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500227/ https://www.ncbi.nlm.nih.gov/pubmed/28690919 http://dx.doi.org/10.1103/PhysRevX.7.011031 |
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author | Sadegh, Sanaz Higgins, Jenny L. Mannion, Patrick C. Tamkun, Michael M. Krapf, Diego |
author_facet | Sadegh, Sanaz Higgins, Jenny L. Mannion, Patrick C. Tamkun, Michael M. Krapf, Diego |
author_sort | Sadegh, Sanaz |
collection | PubMed |
description | A broad range of membrane proteins display anomalous diffusion on the cell surface. Different methods provide evidence for obstructed subdiffusion and diffusion on a fractal space, but the underlying structure inducing anomalous diffusion has never been visualized because of experimental challenges. We addressed this problem by imaging the cortical actin at high resolution while simultaneously tracking individual membrane proteins in live mammalian cells. Our data confirm that actin introduces barriers leading to compartmentalization of the plasma membrane and that membrane proteins are transiently confined within actin fences. Furthermore, superresolution imaging shows that the cortical actin is organized into a self-similar meshwork. These results present a hierarchical nanoscale picture of the plasma membrane. |
format | Online Article Text |
id | pubmed-5500227 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
record_format | MEDLINE/PubMed |
spelling | pubmed-55002272017-07-06 Plasma Membrane is Compartmentalized by a Self-Similar Cortical Actin Meshwork Sadegh, Sanaz Higgins, Jenny L. Mannion, Patrick C. Tamkun, Michael M. Krapf, Diego Phys Rev X Article A broad range of membrane proteins display anomalous diffusion on the cell surface. Different methods provide evidence for obstructed subdiffusion and diffusion on a fractal space, but the underlying structure inducing anomalous diffusion has never been visualized because of experimental challenges. We addressed this problem by imaging the cortical actin at high resolution while simultaneously tracking individual membrane proteins in live mammalian cells. Our data confirm that actin introduces barriers leading to compartmentalization of the plasma membrane and that membrane proteins are transiently confined within actin fences. Furthermore, superresolution imaging shows that the cortical actin is organized into a self-similar meshwork. These results present a hierarchical nanoscale picture of the plasma membrane. 2017-03-09 2017 /pmc/articles/PMC5500227/ /pubmed/28690919 http://dx.doi.org/10.1103/PhysRevX.7.011031 Text en Published by the American Physical Society under the terms of the Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/) license. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. |
spellingShingle | Article Sadegh, Sanaz Higgins, Jenny L. Mannion, Patrick C. Tamkun, Michael M. Krapf, Diego Plasma Membrane is Compartmentalized by a Self-Similar Cortical Actin Meshwork |
title | Plasma Membrane is Compartmentalized by a Self-Similar Cortical Actin Meshwork |
title_full | Plasma Membrane is Compartmentalized by a Self-Similar Cortical Actin Meshwork |
title_fullStr | Plasma Membrane is Compartmentalized by a Self-Similar Cortical Actin Meshwork |
title_full_unstemmed | Plasma Membrane is Compartmentalized by a Self-Similar Cortical Actin Meshwork |
title_short | Plasma Membrane is Compartmentalized by a Self-Similar Cortical Actin Meshwork |
title_sort | plasma membrane is compartmentalized by a self-similar cortical actin meshwork |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500227/ https://www.ncbi.nlm.nih.gov/pubmed/28690919 http://dx.doi.org/10.1103/PhysRevX.7.011031 |
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