Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. A population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by (18)F uptake. An in vitro...

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Autores principales: Langford, Seth T., Wiggins, Cody S., Santos, Roque, Hauser, Melinda, Becker, Jeffrey M., Ruggles, Arthur E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500330/
https://www.ncbi.nlm.nih.gov/pubmed/28683074
http://dx.doi.org/10.1371/journal.pone.0180503
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author Langford, Seth T.
Wiggins, Cody S.
Santos, Roque
Hauser, Melinda
Becker, Jeffrey M.
Ruggles, Arthur E.
author_facet Langford, Seth T.
Wiggins, Cody S.
Santos, Roque
Hauser, Melinda
Becker, Jeffrey M.
Ruggles, Arthur E.
author_sort Langford, Seth T.
collection PubMed
description A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. A population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by (18)F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. This work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.
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spelling pubmed-55003302017-07-11 Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking Langford, Seth T. Wiggins, Cody S. Santos, Roque Hauser, Melinda Becker, Jeffrey M. Ruggles, Arthur E. PLoS One Research Article A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. A population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by (18)F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. This work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers. Public Library of Science 2017-07-06 /pmc/articles/PMC5500330/ /pubmed/28683074 http://dx.doi.org/10.1371/journal.pone.0180503 Text en © 2017 Langford et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Langford, Seth T.
Wiggins, Cody S.
Santos, Roque
Hauser, Melinda
Becker, Jeffrey M.
Ruggles, Arthur E.
Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking
title Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking
title_full Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking
title_fullStr Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking
title_full_unstemmed Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking
title_short Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking
title_sort three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500330/
https://www.ncbi.nlm.nih.gov/pubmed/28683074
http://dx.doi.org/10.1371/journal.pone.0180503
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