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Biodegradation and biodetoxification of Fusarium mycotoxins by Sphaerodes mycoparasitica

A fungus Sphaerodes mycoparasitica SMCD 2220-01 is a host specific mycoparasite against plant pathogenic Fusarium species. Fusarium spp. are producing a plethora of mycotoxins including zearalenone (ZEN), deoxynivalenol (DON) and its acetylated derivatives, 3-acetyl-deoxynivalenol (3-ADON) and 15-ac...

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Autores principales: Kim, Seon Hwa, Vujanovic, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500597/
https://www.ncbi.nlm.nih.gov/pubmed/28687037
http://dx.doi.org/10.1186/s13568-017-0446-6
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author Kim, Seon Hwa
Vujanovic, Vladimir
author_facet Kim, Seon Hwa
Vujanovic, Vladimir
author_sort Kim, Seon Hwa
collection PubMed
description A fungus Sphaerodes mycoparasitica SMCD 2220-01 is a host specific mycoparasite against plant pathogenic Fusarium species. Fusarium spp. are producing a plethora of mycotoxins including zearalenone (ZEN), deoxynivalenol (DON) and its acetylated derivatives, 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyl-deoxynivalenol (15-ADON). The SMCD 2220-01 strain substantially reduced DON, 3-ADON, 15-ADON, and ZEN production capacity in co-culture system. Degradation and detoxification of the pure mycotoxins were also achieved when exposed to SMCD 2220-01 in shake flasks. The thin layer chromatography (TLC) combined with high performance liquid chromatography–electrospray ionization-high resolution mass spectrometry (HPLC–ESI–HRMS) revealed that the amount of mycotoxins exposed to SMCD 2220-01 was considerably reduced compared to control. ZEN level was decreased by 97%, while zearalenone sulfate ([M−H+SO(3)](−) at m/z 397.1052 C(18)H(21)O(8)S(1)) was detected as a metabolite of ZEN converted to less toxic molecule by the mycoparasite. Further, the mycoparasite appeared to degrade DON, 3-ADON, and 15-ADON by 89, 58, and 72%, respectively. The deoxynivalenol sulfate ([M−COCH(3)+SO(3)−CH(2)O](−) at m/z 345.2300 C(14)H(17)O(8)S(1)) was detected as a less toxic metabolic product of DON and 3-ADON. These findings report the SMCD 2220-01 effectiveness to lower mycotoxins-producing capacities of Fusarium, degrade pure mycotoxins and transform them to less toxic metabolites, opening new opportunities for research and innovation for detoxification of mycotoxins.
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spelling pubmed-55005972017-07-25 Biodegradation and biodetoxification of Fusarium mycotoxins by Sphaerodes mycoparasitica Kim, Seon Hwa Vujanovic, Vladimir AMB Express Original Article A fungus Sphaerodes mycoparasitica SMCD 2220-01 is a host specific mycoparasite against plant pathogenic Fusarium species. Fusarium spp. are producing a plethora of mycotoxins including zearalenone (ZEN), deoxynivalenol (DON) and its acetylated derivatives, 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyl-deoxynivalenol (15-ADON). The SMCD 2220-01 strain substantially reduced DON, 3-ADON, 15-ADON, and ZEN production capacity in co-culture system. Degradation and detoxification of the pure mycotoxins were also achieved when exposed to SMCD 2220-01 in shake flasks. The thin layer chromatography (TLC) combined with high performance liquid chromatography–electrospray ionization-high resolution mass spectrometry (HPLC–ESI–HRMS) revealed that the amount of mycotoxins exposed to SMCD 2220-01 was considerably reduced compared to control. ZEN level was decreased by 97%, while zearalenone sulfate ([M−H+SO(3)](−) at m/z 397.1052 C(18)H(21)O(8)S(1)) was detected as a metabolite of ZEN converted to less toxic molecule by the mycoparasite. Further, the mycoparasite appeared to degrade DON, 3-ADON, and 15-ADON by 89, 58, and 72%, respectively. The deoxynivalenol sulfate ([M−COCH(3)+SO(3)−CH(2)O](−) at m/z 345.2300 C(14)H(17)O(8)S(1)) was detected as a less toxic metabolic product of DON and 3-ADON. These findings report the SMCD 2220-01 effectiveness to lower mycotoxins-producing capacities of Fusarium, degrade pure mycotoxins and transform them to less toxic metabolites, opening new opportunities for research and innovation for detoxification of mycotoxins. Springer Berlin Heidelberg 2017-07-06 /pmc/articles/PMC5500597/ /pubmed/28687037 http://dx.doi.org/10.1186/s13568-017-0446-6 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Kim, Seon Hwa
Vujanovic, Vladimir
Biodegradation and biodetoxification of Fusarium mycotoxins by Sphaerodes mycoparasitica
title Biodegradation and biodetoxification of Fusarium mycotoxins by Sphaerodes mycoparasitica
title_full Biodegradation and biodetoxification of Fusarium mycotoxins by Sphaerodes mycoparasitica
title_fullStr Biodegradation and biodetoxification of Fusarium mycotoxins by Sphaerodes mycoparasitica
title_full_unstemmed Biodegradation and biodetoxification of Fusarium mycotoxins by Sphaerodes mycoparasitica
title_short Biodegradation and biodetoxification of Fusarium mycotoxins by Sphaerodes mycoparasitica
title_sort biodegradation and biodetoxification of fusarium mycotoxins by sphaerodes mycoparasitica
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500597/
https://www.ncbi.nlm.nih.gov/pubmed/28687037
http://dx.doi.org/10.1186/s13568-017-0446-6
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