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A novel pH-sensitive carrier for the delivery of antitumor drugs: histidine-modified auricularia auricular polysaccharide nano-micelles

The study was aimed to design a novel pH-sensitive carrier to deliver antitumor drugs to increase treatment efficiency. Histidine (His)was used to modify auricularia auricular polysaccharide (AAP) by esterification. Proton nuclear magnetic resonance spectrometry was developed to characterize the His...

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Autores principales: Wang, Yingying, Li, Pingfei, Chen, Fen, Jia, Lianqun, Xu, Qihao, Gai, Xiumei, Yu, Yibin, Di, Yan, Zhu, Zhihong, Liang, Yanyao, Liu, Mengqi, Pan, Weisan, Yang, Xinggang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500601/
https://www.ncbi.nlm.nih.gov/pubmed/28684737
http://dx.doi.org/10.1038/s41598-017-04428-8
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author Wang, Yingying
Li, Pingfei
Chen, Fen
Jia, Lianqun
Xu, Qihao
Gai, Xiumei
Yu, Yibin
Di, Yan
Zhu, Zhihong
Liang, Yanyao
Liu, Mengqi
Pan, Weisan
Yang, Xinggang
author_facet Wang, Yingying
Li, Pingfei
Chen, Fen
Jia, Lianqun
Xu, Qihao
Gai, Xiumei
Yu, Yibin
Di, Yan
Zhu, Zhihong
Liang, Yanyao
Liu, Mengqi
Pan, Weisan
Yang, Xinggang
author_sort Wang, Yingying
collection PubMed
description The study was aimed to design a novel pH-sensitive carrier to deliver antitumor drugs to increase treatment efficiency. Histidine (His)was used to modify auricularia auricular polysaccharide (AAP) by esterification. Proton nuclear magnetic resonance spectrometry was developed to characterize the His-AAP carrier and the His-AAP Paclitaxel (PTX) micelles were prepared by self-assembled organic solvent evaporation. The formation of His-AAP PTX micelles was confirmed by dynamic light-scattering, transmission electron microscopy and high performance liquid chromatography. It was found that the His-AAP PTX micelles possessed a spherical morphology with an average diameter of 157.2 nm and an 80.3% PTX encapsulation efficiency. In vitro release at pH 7.4, 6.5, 5.0 reached 70%, 71%, and 88%, respectively. The cell viability assay and confocal laser scanning microscope were used to evaluate the cytotoxicity and cell uptake of the His-AAP PTX micelles. Compared with Taxol, the IC(50) of the His-AAP PTX micelles were lower after incubating for 24 h, 48 h, or 72 h (0.216 versus 0.199, 0.065 versus 0.060, and 0.023 versus 0.005, respectively). In a test of tumor-bearing mice, the His-AAP PTX micelles significantly inhibited tumor growth. These results showed that His-AAP PTX micelles are a highly promising therapeutic system for anticancer therapy.
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spelling pubmed-55006012017-07-10 A novel pH-sensitive carrier for the delivery of antitumor drugs: histidine-modified auricularia auricular polysaccharide nano-micelles Wang, Yingying Li, Pingfei Chen, Fen Jia, Lianqun Xu, Qihao Gai, Xiumei Yu, Yibin Di, Yan Zhu, Zhihong Liang, Yanyao Liu, Mengqi Pan, Weisan Yang, Xinggang Sci Rep Article The study was aimed to design a novel pH-sensitive carrier to deliver antitumor drugs to increase treatment efficiency. Histidine (His)was used to modify auricularia auricular polysaccharide (AAP) by esterification. Proton nuclear magnetic resonance spectrometry was developed to characterize the His-AAP carrier and the His-AAP Paclitaxel (PTX) micelles were prepared by self-assembled organic solvent evaporation. The formation of His-AAP PTX micelles was confirmed by dynamic light-scattering, transmission electron microscopy and high performance liquid chromatography. It was found that the His-AAP PTX micelles possessed a spherical morphology with an average diameter of 157.2 nm and an 80.3% PTX encapsulation efficiency. In vitro release at pH 7.4, 6.5, 5.0 reached 70%, 71%, and 88%, respectively. The cell viability assay and confocal laser scanning microscope were used to evaluate the cytotoxicity and cell uptake of the His-AAP PTX micelles. Compared with Taxol, the IC(50) of the His-AAP PTX micelles were lower after incubating for 24 h, 48 h, or 72 h (0.216 versus 0.199, 0.065 versus 0.060, and 0.023 versus 0.005, respectively). In a test of tumor-bearing mice, the His-AAP PTX micelles significantly inhibited tumor growth. These results showed that His-AAP PTX micelles are a highly promising therapeutic system for anticancer therapy. Nature Publishing Group UK 2017-07-06 /pmc/articles/PMC5500601/ /pubmed/28684737 http://dx.doi.org/10.1038/s41598-017-04428-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Yingying
Li, Pingfei
Chen, Fen
Jia, Lianqun
Xu, Qihao
Gai, Xiumei
Yu, Yibin
Di, Yan
Zhu, Zhihong
Liang, Yanyao
Liu, Mengqi
Pan, Weisan
Yang, Xinggang
A novel pH-sensitive carrier for the delivery of antitumor drugs: histidine-modified auricularia auricular polysaccharide nano-micelles
title A novel pH-sensitive carrier for the delivery of antitumor drugs: histidine-modified auricularia auricular polysaccharide nano-micelles
title_full A novel pH-sensitive carrier for the delivery of antitumor drugs: histidine-modified auricularia auricular polysaccharide nano-micelles
title_fullStr A novel pH-sensitive carrier for the delivery of antitumor drugs: histidine-modified auricularia auricular polysaccharide nano-micelles
title_full_unstemmed A novel pH-sensitive carrier for the delivery of antitumor drugs: histidine-modified auricularia auricular polysaccharide nano-micelles
title_short A novel pH-sensitive carrier for the delivery of antitumor drugs: histidine-modified auricularia auricular polysaccharide nano-micelles
title_sort novel ph-sensitive carrier for the delivery of antitumor drugs: histidine-modified auricularia auricular polysaccharide nano-micelles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500601/
https://www.ncbi.nlm.nih.gov/pubmed/28684737
http://dx.doi.org/10.1038/s41598-017-04428-8
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