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Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line

Biological effects of rutin bioactive are limited due to its poor oral bioavailability and its degradation in aqueous environments. For the purpose of bioenhancement, different nanoemulsion systems of rutin were developed by aqueous titration method using water as dispersion media. The nanoemulsion...

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Detalles Bibliográficos
Autores principales: Ahmad, Mohammad, Sahabjada, -, Akhtar, Juber, Hussain, Arshad, Badaruddeen, -, Arshad, Md, Mishra, Anuradha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Leibniz Research Centre for Working Environment and Human Factors 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500835/
https://www.ncbi.nlm.nih.gov/pubmed/28694767
http://dx.doi.org/10.17179/excli2016-668
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author Ahmad, Mohammad
Sahabjada, -
Akhtar, Juber
Hussain, Arshad
Badaruddeen, -
Arshad, Md
Mishra, Anuradha
author_facet Ahmad, Mohammad
Sahabjada, -
Akhtar, Juber
Hussain, Arshad
Badaruddeen, -
Arshad, Md
Mishra, Anuradha
author_sort Ahmad, Mohammad
collection PubMed
description Biological effects of rutin bioactive are limited due to its poor oral bioavailability and its degradation in aqueous environments. For the purpose of bioenhancement, different nanoemulsion systems of rutin were developed by aqueous titration method using water as dispersion media. The nanoemulsion systems were characterized for surface morphology, droplet size, polydispersity index, zeta potential, in vitro release profile and the formulations were optimized. The anticancer potential of optimized nanoemulsion was evaluated by cells viability (MTT) assay, nuclear condensation, and ROS activity using human prostate cancer (PC3) cell line. On the basis of cell viability data the inhibitory concentration (IC(50)) value for optimized nanoemulsion formulation on PC3 cancer cells was found to be 11.8 μM. Fluorescent microscopic analysis and intracellular ROS generation demonstrated significant ROS induction that might lead to triggering the apoptosis pathway. In conclusion, developed nanoemulsion displayed significant efficacy against prostate carcinoma cells.
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spelling pubmed-55008352017-07-10 Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line Ahmad, Mohammad Sahabjada, - Akhtar, Juber Hussain, Arshad Badaruddeen, - Arshad, Md Mishra, Anuradha EXCLI J Original Article Biological effects of rutin bioactive are limited due to its poor oral bioavailability and its degradation in aqueous environments. For the purpose of bioenhancement, different nanoemulsion systems of rutin were developed by aqueous titration method using water as dispersion media. The nanoemulsion systems were characterized for surface morphology, droplet size, polydispersity index, zeta potential, in vitro release profile and the formulations were optimized. The anticancer potential of optimized nanoemulsion was evaluated by cells viability (MTT) assay, nuclear condensation, and ROS activity using human prostate cancer (PC3) cell line. On the basis of cell viability data the inhibitory concentration (IC(50)) value for optimized nanoemulsion formulation on PC3 cancer cells was found to be 11.8 μM. Fluorescent microscopic analysis and intracellular ROS generation demonstrated significant ROS induction that might lead to triggering the apoptosis pathway. In conclusion, developed nanoemulsion displayed significant efficacy against prostate carcinoma cells. Leibniz Research Centre for Working Environment and Human Factors 2017-05-31 /pmc/articles/PMC5500835/ /pubmed/28694767 http://dx.doi.org/10.17179/excli2016-668 Text en Copyright © 2017 Ahmad et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited.
spellingShingle Original Article
Ahmad, Mohammad
Sahabjada, -
Akhtar, Juber
Hussain, Arshad
Badaruddeen, -
Arshad, Md
Mishra, Anuradha
Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line
title Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line
title_full Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line
title_fullStr Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line
title_full_unstemmed Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line
title_short Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line
title_sort development of a new rutin nanoemulsion and its application on prostate carcinoma pc3 cell line
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500835/
https://www.ncbi.nlm.nih.gov/pubmed/28694767
http://dx.doi.org/10.17179/excli2016-668
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