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Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line
Biological effects of rutin bioactive are limited due to its poor oral bioavailability and its degradation in aqueous environments. For the purpose of bioenhancement, different nanoemulsion systems of rutin were developed by aqueous titration method using water as dispersion media. The nanoemulsion...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Leibniz Research Centre for Working Environment and Human Factors
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500835/ https://www.ncbi.nlm.nih.gov/pubmed/28694767 http://dx.doi.org/10.17179/excli2016-668 |
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author | Ahmad, Mohammad Sahabjada, - Akhtar, Juber Hussain, Arshad Badaruddeen, - Arshad, Md Mishra, Anuradha |
author_facet | Ahmad, Mohammad Sahabjada, - Akhtar, Juber Hussain, Arshad Badaruddeen, - Arshad, Md Mishra, Anuradha |
author_sort | Ahmad, Mohammad |
collection | PubMed |
description | Biological effects of rutin bioactive are limited due to its poor oral bioavailability and its degradation in aqueous environments. For the purpose of bioenhancement, different nanoemulsion systems of rutin were developed by aqueous titration method using water as dispersion media. The nanoemulsion systems were characterized for surface morphology, droplet size, polydispersity index, zeta potential, in vitro release profile and the formulations were optimized. The anticancer potential of optimized nanoemulsion was evaluated by cells viability (MTT) assay, nuclear condensation, and ROS activity using human prostate cancer (PC3) cell line. On the basis of cell viability data the inhibitory concentration (IC(50)) value for optimized nanoemulsion formulation on PC3 cancer cells was found to be 11.8 μM. Fluorescent microscopic analysis and intracellular ROS generation demonstrated significant ROS induction that might lead to triggering the apoptosis pathway. In conclusion, developed nanoemulsion displayed significant efficacy against prostate carcinoma cells. |
format | Online Article Text |
id | pubmed-5500835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Leibniz Research Centre for Working Environment and Human Factors |
record_format | MEDLINE/PubMed |
spelling | pubmed-55008352017-07-10 Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line Ahmad, Mohammad Sahabjada, - Akhtar, Juber Hussain, Arshad Badaruddeen, - Arshad, Md Mishra, Anuradha EXCLI J Original Article Biological effects of rutin bioactive are limited due to its poor oral bioavailability and its degradation in aqueous environments. For the purpose of bioenhancement, different nanoemulsion systems of rutin were developed by aqueous titration method using water as dispersion media. The nanoemulsion systems were characterized for surface morphology, droplet size, polydispersity index, zeta potential, in vitro release profile and the formulations were optimized. The anticancer potential of optimized nanoemulsion was evaluated by cells viability (MTT) assay, nuclear condensation, and ROS activity using human prostate cancer (PC3) cell line. On the basis of cell viability data the inhibitory concentration (IC(50)) value for optimized nanoemulsion formulation on PC3 cancer cells was found to be 11.8 μM. Fluorescent microscopic analysis and intracellular ROS generation demonstrated significant ROS induction that might lead to triggering the apoptosis pathway. In conclusion, developed nanoemulsion displayed significant efficacy against prostate carcinoma cells. Leibniz Research Centre for Working Environment and Human Factors 2017-05-31 /pmc/articles/PMC5500835/ /pubmed/28694767 http://dx.doi.org/10.17179/excli2016-668 Text en Copyright © 2017 Ahmad et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited. |
spellingShingle | Original Article Ahmad, Mohammad Sahabjada, - Akhtar, Juber Hussain, Arshad Badaruddeen, - Arshad, Md Mishra, Anuradha Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line |
title | Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line |
title_full | Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line |
title_fullStr | Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line |
title_full_unstemmed | Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line |
title_short | Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line |
title_sort | development of a new rutin nanoemulsion and its application on prostate carcinoma pc3 cell line |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500835/ https://www.ncbi.nlm.nih.gov/pubmed/28694767 http://dx.doi.org/10.17179/excli2016-668 |
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