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Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India

BACKGROUND & OBJECTIVES: Mixed-phenotype acute leukaemia (MPAL) is a rare neoplasm with no definite treatment protocols and a distinctly poor outcome. Advancement in polychromatic flow cytometry has made its identification easier. This prospective study was designed to identify cases of MPAL and...

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Autores principales: Sharma, Manupriya, Sachdeva, Man Updesh Singh, Bose, Parveen, Varma, Neelam, Varma, Subhash, Marwaha, R.K., Malhotra, Pankaj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501054/
https://www.ncbi.nlm.nih.gov/pubmed/28639598
http://dx.doi.org/10.4103/ijmr.IJMR_324_14
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author Sharma, Manupriya
Sachdeva, Man Updesh Singh
Bose, Parveen
Varma, Neelam
Varma, Subhash
Marwaha, R.K.
Malhotra, Pankaj
author_facet Sharma, Manupriya
Sachdeva, Man Updesh Singh
Bose, Parveen
Varma, Neelam
Varma, Subhash
Marwaha, R.K.
Malhotra, Pankaj
author_sort Sharma, Manupriya
collection PubMed
description BACKGROUND & OBJECTIVES: Mixed-phenotype acute leukaemia (MPAL) is a rare neoplasm with no definite treatment protocols and a distinctly poor outcome. Advancement in polychromatic flow cytometry has made its identification easier. This prospective study was designed to identify cases of MPAL and study their clinical presentation and haematological profile in a tertiary care hospital in north India. METHODS: Ethylenediaminetetraacetic acid (EDTA)-anticoagulated bone marrow aspirate samples of patients diagnosed as acute leukaemia (AL) on the basis of morphology were utilized for immunophenotyping. A comprehensive panel of fluorochrome-labelled monoclonal antibodies targeting myeloid, B-cell, T-cell and immaturity markers was utilized. The patients diagnosed to have MPAL, on the basis of the World Health Organization 2008 classification, were selected for further analyses. RESULTS: There were 15 (2.99%) patients with MPAL of the total 501 cases of AL. Seven were children, all males and mean age of 5.08±3.88 yr. Eight were adults, male:female=6:2 and mean age of 21.43±5.74 yr. Eight were diagnosed as B/myeloid and seven were T/myeloid. No association was observed between age and immunophenotype of MPAL. On morphology, 11 were diagnosed as AML and four as ALL, and no specific morphology of blasts was predictive of a MPAL. INTERPRETATION & CONCLUSIONS: MPAL appeared to be a rare neoplasm (2.99% of AL cases). A comprehensive primary panel of monoclonal antibodies should be used to identify this neoplasm known to have a poor outcome.
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spelling pubmed-55010542017-07-13 Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India Sharma, Manupriya Sachdeva, Man Updesh Singh Bose, Parveen Varma, Neelam Varma, Subhash Marwaha, R.K. Malhotra, Pankaj Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Mixed-phenotype acute leukaemia (MPAL) is a rare neoplasm with no definite treatment protocols and a distinctly poor outcome. Advancement in polychromatic flow cytometry has made its identification easier. This prospective study was designed to identify cases of MPAL and study their clinical presentation and haematological profile in a tertiary care hospital in north India. METHODS: Ethylenediaminetetraacetic acid (EDTA)-anticoagulated bone marrow aspirate samples of patients diagnosed as acute leukaemia (AL) on the basis of morphology were utilized for immunophenotyping. A comprehensive panel of fluorochrome-labelled monoclonal antibodies targeting myeloid, B-cell, T-cell and immaturity markers was utilized. The patients diagnosed to have MPAL, on the basis of the World Health Organization 2008 classification, were selected for further analyses. RESULTS: There were 15 (2.99%) patients with MPAL of the total 501 cases of AL. Seven were children, all males and mean age of 5.08±3.88 yr. Eight were adults, male:female=6:2 and mean age of 21.43±5.74 yr. Eight were diagnosed as B/myeloid and seven were T/myeloid. No association was observed between age and immunophenotype of MPAL. On morphology, 11 were diagnosed as AML and four as ALL, and no specific morphology of blasts was predictive of a MPAL. INTERPRETATION & CONCLUSIONS: MPAL appeared to be a rare neoplasm (2.99% of AL cases). A comprehensive primary panel of monoclonal antibodies should be used to identify this neoplasm known to have a poor outcome. Medknow Publications & Media Pvt Ltd 2017-02 /pmc/articles/PMC5501054/ /pubmed/28639598 http://dx.doi.org/10.4103/ijmr.IJMR_324_14 Text en Copyright: © 2017 Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Sharma, Manupriya
Sachdeva, Man Updesh Singh
Bose, Parveen
Varma, Neelam
Varma, Subhash
Marwaha, R.K.
Malhotra, Pankaj
Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India
title Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India
title_full Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India
title_fullStr Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India
title_full_unstemmed Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India
title_short Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India
title_sort haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north india
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501054/
https://www.ncbi.nlm.nih.gov/pubmed/28639598
http://dx.doi.org/10.4103/ijmr.IJMR_324_14
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