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Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India
BACKGROUND & OBJECTIVES: Mixed-phenotype acute leukaemia (MPAL) is a rare neoplasm with no definite treatment protocols and a distinctly poor outcome. Advancement in polychromatic flow cytometry has made its identification easier. This prospective study was designed to identify cases of MPAL and...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501054/ https://www.ncbi.nlm.nih.gov/pubmed/28639598 http://dx.doi.org/10.4103/ijmr.IJMR_324_14 |
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author | Sharma, Manupriya Sachdeva, Man Updesh Singh Bose, Parveen Varma, Neelam Varma, Subhash Marwaha, R.K. Malhotra, Pankaj |
author_facet | Sharma, Manupriya Sachdeva, Man Updesh Singh Bose, Parveen Varma, Neelam Varma, Subhash Marwaha, R.K. Malhotra, Pankaj |
author_sort | Sharma, Manupriya |
collection | PubMed |
description | BACKGROUND & OBJECTIVES: Mixed-phenotype acute leukaemia (MPAL) is a rare neoplasm with no definite treatment protocols and a distinctly poor outcome. Advancement in polychromatic flow cytometry has made its identification easier. This prospective study was designed to identify cases of MPAL and study their clinical presentation and haematological profile in a tertiary care hospital in north India. METHODS: Ethylenediaminetetraacetic acid (EDTA)-anticoagulated bone marrow aspirate samples of patients diagnosed as acute leukaemia (AL) on the basis of morphology were utilized for immunophenotyping. A comprehensive panel of fluorochrome-labelled monoclonal antibodies targeting myeloid, B-cell, T-cell and immaturity markers was utilized. The patients diagnosed to have MPAL, on the basis of the World Health Organization 2008 classification, were selected for further analyses. RESULTS: There were 15 (2.99%) patients with MPAL of the total 501 cases of AL. Seven were children, all males and mean age of 5.08±3.88 yr. Eight were adults, male:female=6:2 and mean age of 21.43±5.74 yr. Eight were diagnosed as B/myeloid and seven were T/myeloid. No association was observed between age and immunophenotype of MPAL. On morphology, 11 were diagnosed as AML and four as ALL, and no specific morphology of blasts was predictive of a MPAL. INTERPRETATION & CONCLUSIONS: MPAL appeared to be a rare neoplasm (2.99% of AL cases). A comprehensive primary panel of monoclonal antibodies should be used to identify this neoplasm known to have a poor outcome. |
format | Online Article Text |
id | pubmed-5501054 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-55010542017-07-13 Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India Sharma, Manupriya Sachdeva, Man Updesh Singh Bose, Parveen Varma, Neelam Varma, Subhash Marwaha, R.K. Malhotra, Pankaj Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Mixed-phenotype acute leukaemia (MPAL) is a rare neoplasm with no definite treatment protocols and a distinctly poor outcome. Advancement in polychromatic flow cytometry has made its identification easier. This prospective study was designed to identify cases of MPAL and study their clinical presentation and haematological profile in a tertiary care hospital in north India. METHODS: Ethylenediaminetetraacetic acid (EDTA)-anticoagulated bone marrow aspirate samples of patients diagnosed as acute leukaemia (AL) on the basis of morphology were utilized for immunophenotyping. A comprehensive panel of fluorochrome-labelled monoclonal antibodies targeting myeloid, B-cell, T-cell and immaturity markers was utilized. The patients diagnosed to have MPAL, on the basis of the World Health Organization 2008 classification, were selected for further analyses. RESULTS: There were 15 (2.99%) patients with MPAL of the total 501 cases of AL. Seven were children, all males and mean age of 5.08±3.88 yr. Eight were adults, male:female=6:2 and mean age of 21.43±5.74 yr. Eight were diagnosed as B/myeloid and seven were T/myeloid. No association was observed between age and immunophenotype of MPAL. On morphology, 11 were diagnosed as AML and four as ALL, and no specific morphology of blasts was predictive of a MPAL. INTERPRETATION & CONCLUSIONS: MPAL appeared to be a rare neoplasm (2.99% of AL cases). A comprehensive primary panel of monoclonal antibodies should be used to identify this neoplasm known to have a poor outcome. Medknow Publications & Media Pvt Ltd 2017-02 /pmc/articles/PMC5501054/ /pubmed/28639598 http://dx.doi.org/10.4103/ijmr.IJMR_324_14 Text en Copyright: © 2017 Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Sharma, Manupriya Sachdeva, Man Updesh Singh Bose, Parveen Varma, Neelam Varma, Subhash Marwaha, R.K. Malhotra, Pankaj Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India |
title | Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India |
title_full | Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India |
title_fullStr | Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India |
title_full_unstemmed | Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India |
title_short | Haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north India |
title_sort | haematological profile of patients with mixed-phenotype acute leukaemia from a tertiary care centre of north india |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501054/ https://www.ncbi.nlm.nih.gov/pubmed/28639598 http://dx.doi.org/10.4103/ijmr.IJMR_324_14 |
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