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Interferon γ induced compositional changes in human bone marrow derived mesenchymal stem/stromal cells
BACKGROUND: Mesenchymal stem/stromal cells (MSC) display a range of immunoregulatory properties which can be enhanced by the exposure to cytokines such interferon γ (IFN-γ). However the compositional changes associated with the ‘licensing’ of these cells have not been clearly defined. The present st...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501357/ https://www.ncbi.nlm.nih.gov/pubmed/28694743 http://dx.doi.org/10.1186/s12014-017-9161-1 |
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author | Guan, Qingdong Ezzati, Peyman Spicer, Victor Krokhin, Oleg Wall, Donna Wilkins, John A. |
author_facet | Guan, Qingdong Ezzati, Peyman Spicer, Victor Krokhin, Oleg Wall, Donna Wilkins, John A. |
author_sort | Guan, Qingdong |
collection | PubMed |
description | BACKGROUND: Mesenchymal stem/stromal cells (MSC) display a range of immunoregulatory properties which can be enhanced by the exposure to cytokines such interferon γ (IFN-γ). However the compositional changes associated with the ‘licensing’ of these cells have not been clearly defined. The present study was undertaken to provide a detailed comparative proteomic analysis of the compositional changes that occur in human bone marrow derived MSC following 20 h treatment with IFN-γ. METHODS: 2D LC MSMS analysis of control and IFN-γ treated cells from 5 different healthy donors provided confident identification of more than 8400 proteins. RESULTS: In total 210 proteins were shown to be significantly altered in their expression levels (≥|2SD|) following IFN-γ treatment. The changes for several of these proteins were confirmed by flow cytometry. STRING analysis determined that approximately 30% of the altered proteins physically interacted in described interferon mediated processes. Comparison of the list of proteins that were identified as changed in the proteomic analysis with data for the same proteins in the Interferome DB indicated that ~35% of these proteins have not been reported to be IFN-γ responsive in a range of cell types. CONCLUSIONS: This data provides an in depth analysis of the proteome of basal and IFN-γ treated human mesenchymal stem cells and it identifies a number of novel proteins that may contribute to the immunoregulatory capacity if IFN-γ licensed cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12014-017-9161-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5501357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-55013572017-07-10 Interferon γ induced compositional changes in human bone marrow derived mesenchymal stem/stromal cells Guan, Qingdong Ezzati, Peyman Spicer, Victor Krokhin, Oleg Wall, Donna Wilkins, John A. Clin Proteomics Research BACKGROUND: Mesenchymal stem/stromal cells (MSC) display a range of immunoregulatory properties which can be enhanced by the exposure to cytokines such interferon γ (IFN-γ). However the compositional changes associated with the ‘licensing’ of these cells have not been clearly defined. The present study was undertaken to provide a detailed comparative proteomic analysis of the compositional changes that occur in human bone marrow derived MSC following 20 h treatment with IFN-γ. METHODS: 2D LC MSMS analysis of control and IFN-γ treated cells from 5 different healthy donors provided confident identification of more than 8400 proteins. RESULTS: In total 210 proteins were shown to be significantly altered in their expression levels (≥|2SD|) following IFN-γ treatment. The changes for several of these proteins were confirmed by flow cytometry. STRING analysis determined that approximately 30% of the altered proteins physically interacted in described interferon mediated processes. Comparison of the list of proteins that were identified as changed in the proteomic analysis with data for the same proteins in the Interferome DB indicated that ~35% of these proteins have not been reported to be IFN-γ responsive in a range of cell types. CONCLUSIONS: This data provides an in depth analysis of the proteome of basal and IFN-γ treated human mesenchymal stem cells and it identifies a number of novel proteins that may contribute to the immunoregulatory capacity if IFN-γ licensed cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12014-017-9161-1) contains supplementary material, which is available to authorized users. BioMed Central 2017-07-06 /pmc/articles/PMC5501357/ /pubmed/28694743 http://dx.doi.org/10.1186/s12014-017-9161-1 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Guan, Qingdong Ezzati, Peyman Spicer, Victor Krokhin, Oleg Wall, Donna Wilkins, John A. Interferon γ induced compositional changes in human bone marrow derived mesenchymal stem/stromal cells |
title | Interferon γ induced compositional changes in human bone marrow derived mesenchymal stem/stromal cells |
title_full | Interferon γ induced compositional changes in human bone marrow derived mesenchymal stem/stromal cells |
title_fullStr | Interferon γ induced compositional changes in human bone marrow derived mesenchymal stem/stromal cells |
title_full_unstemmed | Interferon γ induced compositional changes in human bone marrow derived mesenchymal stem/stromal cells |
title_short | Interferon γ induced compositional changes in human bone marrow derived mesenchymal stem/stromal cells |
title_sort | interferon γ induced compositional changes in human bone marrow derived mesenchymal stem/stromal cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501357/ https://www.ncbi.nlm.nih.gov/pubmed/28694743 http://dx.doi.org/10.1186/s12014-017-9161-1 |
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