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Microstructural Crimp of the Lamina Cribrosa and Peripapillary Sclera Collagen Fibers

PURPOSE: Although collagen microstructural crimp is a major determinant of ocular biomechanics, no direct measurements of optic nerve head (ONH) crimp have been reported. Our goal was to characterize the crimp period of the lamina cribrosa (LC) and peripapillary sclera (PPS) at low and normal IOPs....

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Autores principales: Jan, Ning-Jiun, Gomez, Celeste, Moed, Saundria, Voorhees, Andrew P., Schuman, Joel S., Bilonick, Richard A., Sigal, Ian A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501496/
https://www.ncbi.nlm.nih.gov/pubmed/28687851
http://dx.doi.org/10.1167/iovs.17-21811
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author Jan, Ning-Jiun
Gomez, Celeste
Moed, Saundria
Voorhees, Andrew P.
Schuman, Joel S.
Bilonick, Richard A.
Sigal, Ian A.
author_facet Jan, Ning-Jiun
Gomez, Celeste
Moed, Saundria
Voorhees, Andrew P.
Schuman, Joel S.
Bilonick, Richard A.
Sigal, Ian A.
author_sort Jan, Ning-Jiun
collection PubMed
description PURPOSE: Although collagen microstructural crimp is a major determinant of ocular biomechanics, no direct measurements of optic nerve head (ONH) crimp have been reported. Our goal was to characterize the crimp period of the lamina cribrosa (LC) and peripapillary sclera (PPS) at low and normal IOPs. METHODS: ONHs from 11 sheep eyes were fixed at 10-, 5-, or 0-mm Hg IOP and crimp periods measured manually from coronal cryosections imaged with polarized light microscopy (PLM). Using linear mixed-effect models, we characterized the LC and PPS periods, and how they varied with distance from the scleral canal edge. RESULTS: A total of 17,374 manual collagen crimp period measurements were obtained with high repeatability (1.9 μm) and reproducibility (4.7 μm). The periods were smaller (P < 0.001) and less variable in the LC than in the PPS: average (SD) of 13.8 (3.1) μm in the LC, and 31.0 (10.4) μm in the PPS. LC crimp period did not vary with distance from the scleral canal wall (P > 0.1). PPS period increased with the square root of the distance to the canal (P < 0.0001). CONCLUSIONS: Small, uniform crimp periods within the sheep LC and immediately adjacent PPS may indicate that these tissues are setup to prevent large or heterogeneous deformations that insult the neural tissues within the canal. An increasing more variable period with distance from the canal provides a smooth transition of mechanical properties that minimizes stress and strain concentrations.
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spelling pubmed-55014962017-07-10 Microstructural Crimp of the Lamina Cribrosa and Peripapillary Sclera Collagen Fibers Jan, Ning-Jiun Gomez, Celeste Moed, Saundria Voorhees, Andrew P. Schuman, Joel S. Bilonick, Richard A. Sigal, Ian A. Invest Ophthalmol Vis Sci Glaucoma PURPOSE: Although collagen microstructural crimp is a major determinant of ocular biomechanics, no direct measurements of optic nerve head (ONH) crimp have been reported. Our goal was to characterize the crimp period of the lamina cribrosa (LC) and peripapillary sclera (PPS) at low and normal IOPs. METHODS: ONHs from 11 sheep eyes were fixed at 10-, 5-, or 0-mm Hg IOP and crimp periods measured manually from coronal cryosections imaged with polarized light microscopy (PLM). Using linear mixed-effect models, we characterized the LC and PPS periods, and how they varied with distance from the scleral canal edge. RESULTS: A total of 17,374 manual collagen crimp period measurements were obtained with high repeatability (1.9 μm) and reproducibility (4.7 μm). The periods were smaller (P < 0.001) and less variable in the LC than in the PPS: average (SD) of 13.8 (3.1) μm in the LC, and 31.0 (10.4) μm in the PPS. LC crimp period did not vary with distance from the scleral canal wall (P > 0.1). PPS period increased with the square root of the distance to the canal (P < 0.0001). CONCLUSIONS: Small, uniform crimp periods within the sheep LC and immediately adjacent PPS may indicate that these tissues are setup to prevent large or heterogeneous deformations that insult the neural tissues within the canal. An increasing more variable period with distance from the canal provides a smooth transition of mechanical properties that minimizes stress and strain concentrations. The Association for Research in Vision and Ophthalmology 2017-07 /pmc/articles/PMC5501496/ /pubmed/28687851 http://dx.doi.org/10.1167/iovs.17-21811 Text en Copyright 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Glaucoma
Jan, Ning-Jiun
Gomez, Celeste
Moed, Saundria
Voorhees, Andrew P.
Schuman, Joel S.
Bilonick, Richard A.
Sigal, Ian A.
Microstructural Crimp of the Lamina Cribrosa and Peripapillary Sclera Collagen Fibers
title Microstructural Crimp of the Lamina Cribrosa and Peripapillary Sclera Collagen Fibers
title_full Microstructural Crimp of the Lamina Cribrosa and Peripapillary Sclera Collagen Fibers
title_fullStr Microstructural Crimp of the Lamina Cribrosa and Peripapillary Sclera Collagen Fibers
title_full_unstemmed Microstructural Crimp of the Lamina Cribrosa and Peripapillary Sclera Collagen Fibers
title_short Microstructural Crimp of the Lamina Cribrosa and Peripapillary Sclera Collagen Fibers
title_sort microstructural crimp of the lamina cribrosa and peripapillary sclera collagen fibers
topic Glaucoma
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501496/
https://www.ncbi.nlm.nih.gov/pubmed/28687851
http://dx.doi.org/10.1167/iovs.17-21811
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