Identification and characterization of Burkholderia multivorans CCA53

OBJECTIVE: A lignin-degrading bacterium, Burkholderia sp. CCA53, was previously isolated from leaf soil. The purpose of this study was to determine phenotypic and biochemical features of Burkholderia sp. CCA53. RESULTS: Multilocus sequence typing (MLST) analysis based on fragments of the atpD, gltD,...

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Autores principales: Akita, Hironaga, Kimura, Zen-ichiro, Yusoff, Mohd Zulkhairi Mohd, Nakashima, Nobutaka, Hoshino, Tamotsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501517/
https://www.ncbi.nlm.nih.gov/pubmed/28683814
http://dx.doi.org/10.1186/s13104-017-2565-1
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author Akita, Hironaga
Kimura, Zen-ichiro
Yusoff, Mohd Zulkhairi Mohd
Nakashima, Nobutaka
Hoshino, Tamotsu
author_facet Akita, Hironaga
Kimura, Zen-ichiro
Yusoff, Mohd Zulkhairi Mohd
Nakashima, Nobutaka
Hoshino, Tamotsu
author_sort Akita, Hironaga
collection PubMed
description OBJECTIVE: A lignin-degrading bacterium, Burkholderia sp. CCA53, was previously isolated from leaf soil. The purpose of this study was to determine phenotypic and biochemical features of Burkholderia sp. CCA53. RESULTS: Multilocus sequence typing (MLST) analysis based on fragments of the atpD, gltD, gyrB, lepA, recA and trpB gene sequences was performed to identify Burkholderia sp. CCA53. The MLST analysis revealed that Burkholderia sp. CCA53 was tightly clustered with B. multivorans ATCC BAA-247(T). The quinone and cellular fatty acid profiles, carbon source utilization, growth temperature and pH were consistent with the characteristics of B. multivorans species. Burkholderia sp. CCA53 was therefore identified as B. multivorans CCA53.
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spelling pubmed-55015172017-07-10 Identification and characterization of Burkholderia multivorans CCA53 Akita, Hironaga Kimura, Zen-ichiro Yusoff, Mohd Zulkhairi Mohd Nakashima, Nobutaka Hoshino, Tamotsu BMC Res Notes Research Note OBJECTIVE: A lignin-degrading bacterium, Burkholderia sp. CCA53, was previously isolated from leaf soil. The purpose of this study was to determine phenotypic and biochemical features of Burkholderia sp. CCA53. RESULTS: Multilocus sequence typing (MLST) analysis based on fragments of the atpD, gltD, gyrB, lepA, recA and trpB gene sequences was performed to identify Burkholderia sp. CCA53. The MLST analysis revealed that Burkholderia sp. CCA53 was tightly clustered with B. multivorans ATCC BAA-247(T). The quinone and cellular fatty acid profiles, carbon source utilization, growth temperature and pH were consistent with the characteristics of B. multivorans species. Burkholderia sp. CCA53 was therefore identified as B. multivorans CCA53. BioMed Central 2017-07-06 /pmc/articles/PMC5501517/ /pubmed/28683814 http://dx.doi.org/10.1186/s13104-017-2565-1 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Akita, Hironaga
Kimura, Zen-ichiro
Yusoff, Mohd Zulkhairi Mohd
Nakashima, Nobutaka
Hoshino, Tamotsu
Identification and characterization of Burkholderia multivorans CCA53
title Identification and characterization of Burkholderia multivorans CCA53
title_full Identification and characterization of Burkholderia multivorans CCA53
title_fullStr Identification and characterization of Burkholderia multivorans CCA53
title_full_unstemmed Identification and characterization of Burkholderia multivorans CCA53
title_short Identification and characterization of Burkholderia multivorans CCA53
title_sort identification and characterization of burkholderia multivorans cca53
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501517/
https://www.ncbi.nlm.nih.gov/pubmed/28683814
http://dx.doi.org/10.1186/s13104-017-2565-1
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