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Microarray expression profile of circular RNAs in chronic thromboembolic pulmonary hypertension
BACKGROUND: Chronic thromboembolic pulmonary hypertension (CTEPH) is a rare but debilitating and life-threatening complication of acute pulmonary embolism. Circular RNAs (circRNAs), presenting as covalently closed continuous loops, are RNA molecules with covalently joined 3′- and 5′-ends formed by b...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502157/ https://www.ncbi.nlm.nih.gov/pubmed/28682884 http://dx.doi.org/10.1097/MD.0000000000007354 |
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author | Miao, Ran Wang, Ying Wan, Jun Leng, Dong Gong, Juanni Li, Jifeng Liang, Yan Zhai, Zhenguo Yang, Yuanhua |
author_facet | Miao, Ran Wang, Ying Wan, Jun Leng, Dong Gong, Juanni Li, Jifeng Liang, Yan Zhai, Zhenguo Yang, Yuanhua |
author_sort | Miao, Ran |
collection | PubMed |
description | BACKGROUND: Chronic thromboembolic pulmonary hypertension (CTEPH) is a rare but debilitating and life-threatening complication of acute pulmonary embolism. Circular RNAs (circRNAs), presenting as covalently closed continuous loops, are RNA molecules with covalently joined 3′- and 5′-ends formed by back-splicing events. circRNAs may be significant biological molecules to understand disease mechanisms and to identify biomarkers for disease diagnosis and therapy. The aim of this study was to investigate the potential roles of circRNAs in CTEPH. METHODS: Ten human blood samples (5 each from CTEPH and control groups) were included in the Agilent circRNA chip. The differentially expressed circRNAs were evaluated using t test, with significance set at a P value of < .05. A functional enrichment analysis for differentially expressed circRNAs was performed using DAVID online tools, and a Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis for target genes of miRNAs was performed using the R package clusterProfiler. Furthermore, miRNAs that interacted with differentially expressed circRNAs were predicted using the miRanda package. mRNAs that had clear biological functions and were regulated by miRNAs were predicted using miRWalk2.0 and then combined into a circRNA–miRNA–mRNA network. RESULTS: In total, 351 differentially expressed circRNAs (122 upregulated and 229 downregulated) between CTEPH and control groups were obtained; among these circRNAs, hsa_circ_0002062 and hsa_circ_0022342 might be important because they can regulate 761 (e.g., hsa-miR-942–5p) and 453 (e.g., hsa-miR-940) miRNAs, respectively. Target genes (e.g., cyclin-dependent kinase 6) of hsa-miR-942–5p were mainly enriched in cancer-related pathways, whereas target genes (e.g., CRK-Like Proto-Oncogene, Adaptor Protein) of hsa-miR-940 were enriched in the ErbB signaling pathway. Therefore, these pathways are potentially important in CTEPH. CONCLUSIONS: Our findings suggested that hsa_circ_0002062 and hsa_circ_0022342 may be key circRNAs for CTEPH development and that their targeted regulation may be an effective approach for treating CTEPH. |
format | Online Article Text |
id | pubmed-5502157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Wolters Kluwer Health |
record_format | MEDLINE/PubMed |
spelling | pubmed-55021572017-07-18 Microarray expression profile of circular RNAs in chronic thromboembolic pulmonary hypertension Miao, Ran Wang, Ying Wan, Jun Leng, Dong Gong, Juanni Li, Jifeng Liang, Yan Zhai, Zhenguo Yang, Yuanhua Medicine (Baltimore) 6700 BACKGROUND: Chronic thromboembolic pulmonary hypertension (CTEPH) is a rare but debilitating and life-threatening complication of acute pulmonary embolism. Circular RNAs (circRNAs), presenting as covalently closed continuous loops, are RNA molecules with covalently joined 3′- and 5′-ends formed by back-splicing events. circRNAs may be significant biological molecules to understand disease mechanisms and to identify biomarkers for disease diagnosis and therapy. The aim of this study was to investigate the potential roles of circRNAs in CTEPH. METHODS: Ten human blood samples (5 each from CTEPH and control groups) were included in the Agilent circRNA chip. The differentially expressed circRNAs were evaluated using t test, with significance set at a P value of < .05. A functional enrichment analysis for differentially expressed circRNAs was performed using DAVID online tools, and a Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis for target genes of miRNAs was performed using the R package clusterProfiler. Furthermore, miRNAs that interacted with differentially expressed circRNAs were predicted using the miRanda package. mRNAs that had clear biological functions and were regulated by miRNAs were predicted using miRWalk2.0 and then combined into a circRNA–miRNA–mRNA network. RESULTS: In total, 351 differentially expressed circRNAs (122 upregulated and 229 downregulated) between CTEPH and control groups were obtained; among these circRNAs, hsa_circ_0002062 and hsa_circ_0022342 might be important because they can regulate 761 (e.g., hsa-miR-942–5p) and 453 (e.g., hsa-miR-940) miRNAs, respectively. Target genes (e.g., cyclin-dependent kinase 6) of hsa-miR-942–5p were mainly enriched in cancer-related pathways, whereas target genes (e.g., CRK-Like Proto-Oncogene, Adaptor Protein) of hsa-miR-940 were enriched in the ErbB signaling pathway. Therefore, these pathways are potentially important in CTEPH. CONCLUSIONS: Our findings suggested that hsa_circ_0002062 and hsa_circ_0022342 may be key circRNAs for CTEPH development and that their targeted regulation may be an effective approach for treating CTEPH. Wolters Kluwer Health 2017-07-07 /pmc/articles/PMC5502157/ /pubmed/28682884 http://dx.doi.org/10.1097/MD.0000000000007354 Text en Copyright © 2017 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0 |
spellingShingle | 6700 Miao, Ran Wang, Ying Wan, Jun Leng, Dong Gong, Juanni Li, Jifeng Liang, Yan Zhai, Zhenguo Yang, Yuanhua Microarray expression profile of circular RNAs in chronic thromboembolic pulmonary hypertension |
title | Microarray expression profile of circular RNAs in chronic thromboembolic pulmonary hypertension |
title_full | Microarray expression profile of circular RNAs in chronic thromboembolic pulmonary hypertension |
title_fullStr | Microarray expression profile of circular RNAs in chronic thromboembolic pulmonary hypertension |
title_full_unstemmed | Microarray expression profile of circular RNAs in chronic thromboembolic pulmonary hypertension |
title_short | Microarray expression profile of circular RNAs in chronic thromboembolic pulmonary hypertension |
title_sort | microarray expression profile of circular rnas in chronic thromboembolic pulmonary hypertension |
topic | 6700 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502157/ https://www.ncbi.nlm.nih.gov/pubmed/28682884 http://dx.doi.org/10.1097/MD.0000000000007354 |
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