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Cytotoxic evaluation of a new ceramic-based root canal sealer on human fibroblasts

OBJECTIVES: The purpose of this study was to evaluate the cytotoxicity of a new bioceramic-based root canal sealer (BioMM) by direct and indirect contact with human fibroblasts and to compare it with a zinc oxide-eugenol sealer, the Pulp Canal Sealer-extended working time (PCS-EWT). MATERIALS AND ME...

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Autores principales: Chakar, Sandra, Changotade, Sylvie, Osta, Nada, Khalil, Issam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502555/
https://www.ncbi.nlm.nih.gov/pubmed/28729783
http://dx.doi.org/10.4103/ejd.ejd_2_17
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author Chakar, Sandra
Changotade, Sylvie
Osta, Nada
Khalil, Issam
author_facet Chakar, Sandra
Changotade, Sylvie
Osta, Nada
Khalil, Issam
author_sort Chakar, Sandra
collection PubMed
description OBJECTIVES: The purpose of this study was to evaluate the cytotoxicity of a new bioceramic-based root canal sealer (BioMM) by direct and indirect contact with human fibroblasts and to compare it with a zinc oxide-eugenol sealer, the Pulp Canal Sealer-extended working time (PCS-EWT). MATERIALS AND METHODS: Cell viability was assessed through direct and indirect contact between human fibroblasts and sealer. Direct contact was performed at 24 h, whereas the indirect contact was performed at 24 and 48 h at different concentrations: 100%, 50%, and 25%. After direct contact, 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used and the optical density was measured by a spectrophotometer. Giemsa stain was also performed for a qualitative evaluation of the cells. STATISTICAL ANALYSIS USED: Shapiro–Wilk test was used to verify the normality of distribution of the variable. Data were analyzed by analysis of variance and Tukey tests using SPSS for Windows software 18.0. The significance level used was P < 0.05. RESULTS: Direct contact showed a significantly higher cell viability with BioMM as compared to PCS-EWT (P = 0.002). Cell viability at 24 h was significantly higher with BioMM compared to PCS-EWT for the concentrations of 50% (P = 0.004) and 25% (P = 0.003), whereas no significant difference was noted at 100% (P = 0.141). Cell viability at 48 h was significantly higher with BioMM as compared to PCS-EWT at 25% (P = 0.007). No significant difference was observed at 100% (P = 0.484) and 50% (P = 0.185). CONCLUSION: BioMM may be considered minimally cytotoxic if accidentally extruded into the periapical tissues.
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spelling pubmed-55025552017-07-20 Cytotoxic evaluation of a new ceramic-based root canal sealer on human fibroblasts Chakar, Sandra Changotade, Sylvie Osta, Nada Khalil, Issam Eur J Dent Original Article OBJECTIVES: The purpose of this study was to evaluate the cytotoxicity of a new bioceramic-based root canal sealer (BioMM) by direct and indirect contact with human fibroblasts and to compare it with a zinc oxide-eugenol sealer, the Pulp Canal Sealer-extended working time (PCS-EWT). MATERIALS AND METHODS: Cell viability was assessed through direct and indirect contact between human fibroblasts and sealer. Direct contact was performed at 24 h, whereas the indirect contact was performed at 24 and 48 h at different concentrations: 100%, 50%, and 25%. After direct contact, 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used and the optical density was measured by a spectrophotometer. Giemsa stain was also performed for a qualitative evaluation of the cells. STATISTICAL ANALYSIS USED: Shapiro–Wilk test was used to verify the normality of distribution of the variable. Data were analyzed by analysis of variance and Tukey tests using SPSS for Windows software 18.0. The significance level used was P < 0.05. RESULTS: Direct contact showed a significantly higher cell viability with BioMM as compared to PCS-EWT (P = 0.002). Cell viability at 24 h was significantly higher with BioMM compared to PCS-EWT for the concentrations of 50% (P = 0.004) and 25% (P = 0.003), whereas no significant difference was noted at 100% (P = 0.141). Cell viability at 48 h was significantly higher with BioMM as compared to PCS-EWT at 25% (P = 0.007). No significant difference was observed at 100% (P = 0.484) and 50% (P = 0.185). CONCLUSION: BioMM may be considered minimally cytotoxic if accidentally extruded into the periapical tissues. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5502555/ /pubmed/28729783 http://dx.doi.org/10.4103/ejd.ejd_2_17 Text en Copyright: © 2017 European Journal of Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Chakar, Sandra
Changotade, Sylvie
Osta, Nada
Khalil, Issam
Cytotoxic evaluation of a new ceramic-based root canal sealer on human fibroblasts
title Cytotoxic evaluation of a new ceramic-based root canal sealer on human fibroblasts
title_full Cytotoxic evaluation of a new ceramic-based root canal sealer on human fibroblasts
title_fullStr Cytotoxic evaluation of a new ceramic-based root canal sealer on human fibroblasts
title_full_unstemmed Cytotoxic evaluation of a new ceramic-based root canal sealer on human fibroblasts
title_short Cytotoxic evaluation of a new ceramic-based root canal sealer on human fibroblasts
title_sort cytotoxic evaluation of a new ceramic-based root canal sealer on human fibroblasts
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502555/
https://www.ncbi.nlm.nih.gov/pubmed/28729783
http://dx.doi.org/10.4103/ejd.ejd_2_17
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