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Rapid Assay to Assess Bacterial Adhesion on Textiles

Textiles are frequently colonized by microorganisms leading to undesired consequences like hygienic problems. Biocidal coatings often raise environmental and health concerns, thus sustainable, biocide-free coatings are of interest. To develop novel anti-adhesive textile coatings, a rapid, reliable,...

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Autores principales: Schmidt-Emrich, Sabrina, Stiefel, Philipp, Rupper, Patrick, Katzenmeier, Heinz, Amberg, Caroline, Maniura-Weber, Katharina, Ren, Qun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502901/
https://www.ncbi.nlm.nih.gov/pubmed/28773373
http://dx.doi.org/10.3390/ma9040249
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author Schmidt-Emrich, Sabrina
Stiefel, Philipp
Rupper, Patrick
Katzenmeier, Heinz
Amberg, Caroline
Maniura-Weber, Katharina
Ren, Qun
author_facet Schmidt-Emrich, Sabrina
Stiefel, Philipp
Rupper, Patrick
Katzenmeier, Heinz
Amberg, Caroline
Maniura-Weber, Katharina
Ren, Qun
author_sort Schmidt-Emrich, Sabrina
collection PubMed
description Textiles are frequently colonized by microorganisms leading to undesired consequences like hygienic problems. Biocidal coatings often raise environmental and health concerns, thus sustainable, biocide-free coatings are of interest. To develop novel anti-adhesive textile coatings, a rapid, reliable, and quantitative high-throughput method to study microbial attachment to fabrics is required, however currently not available. Here, a fast and reliable 96-well plate-based screening method is developed. The quantification of bacterial adhesion is based on nucleic acid staining by SYTO9, with Pseudomonas aeruginosa and Staphylococcus aureus as the model microorganisms. Subsequently, 38 commercially available and novel coatings were evaluated for their anti-bacterial adhesion properties. A poly(l-lysine)-g-poly(ethylene glycol) coating on polyester textile substratum revealed an 80% reduction of bacterial adhesion. Both the coating itself and the anti-adhesive property were stable after 20 washing cycles, confirmed by X-ray analysis. The assay provides an efficient tool to rapidly screen for non-biocidal coatings reducing bacterial attachment.
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spelling pubmed-55029012017-07-28 Rapid Assay to Assess Bacterial Adhesion on Textiles Schmidt-Emrich, Sabrina Stiefel, Philipp Rupper, Patrick Katzenmeier, Heinz Amberg, Caroline Maniura-Weber, Katharina Ren, Qun Materials (Basel) Article Textiles are frequently colonized by microorganisms leading to undesired consequences like hygienic problems. Biocidal coatings often raise environmental and health concerns, thus sustainable, biocide-free coatings are of interest. To develop novel anti-adhesive textile coatings, a rapid, reliable, and quantitative high-throughput method to study microbial attachment to fabrics is required, however currently not available. Here, a fast and reliable 96-well plate-based screening method is developed. The quantification of bacterial adhesion is based on nucleic acid staining by SYTO9, with Pseudomonas aeruginosa and Staphylococcus aureus as the model microorganisms. Subsequently, 38 commercially available and novel coatings were evaluated for their anti-bacterial adhesion properties. A poly(l-lysine)-g-poly(ethylene glycol) coating on polyester textile substratum revealed an 80% reduction of bacterial adhesion. Both the coating itself and the anti-adhesive property were stable after 20 washing cycles, confirmed by X-ray analysis. The assay provides an efficient tool to rapidly screen for non-biocidal coatings reducing bacterial attachment. MDPI 2016-03-30 /pmc/articles/PMC5502901/ /pubmed/28773373 http://dx.doi.org/10.3390/ma9040249 Text en © 2016 by the authors; Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schmidt-Emrich, Sabrina
Stiefel, Philipp
Rupper, Patrick
Katzenmeier, Heinz
Amberg, Caroline
Maniura-Weber, Katharina
Ren, Qun
Rapid Assay to Assess Bacterial Adhesion on Textiles
title Rapid Assay to Assess Bacterial Adhesion on Textiles
title_full Rapid Assay to Assess Bacterial Adhesion on Textiles
title_fullStr Rapid Assay to Assess Bacterial Adhesion on Textiles
title_full_unstemmed Rapid Assay to Assess Bacterial Adhesion on Textiles
title_short Rapid Assay to Assess Bacterial Adhesion on Textiles
title_sort rapid assay to assess bacterial adhesion on textiles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502901/
https://www.ncbi.nlm.nih.gov/pubmed/28773373
http://dx.doi.org/10.3390/ma9040249
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