Cargando…

Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides

This paper investigates the synthesis of paramagnetic nanoparticles, which are able to bind branched chain amino acids (BCAAs)—leucine, valine, and isoleucine and, thus, serve as a tool for their isolation. Further, by this, we present an approach for encapsulation of nanoparticles into a liposome c...

Descripción completa

Detalles Bibliográficos
Autores principales: Vesely, Radek, Jelinkova, Pavlina, Hegerova, Dagmar, Cernei, Natalia, Kopel, Pavel, Moulick, Amitava, Richtera, Lukas, Heger, Zbynek, Adam, Vojtech, Zitka, Ondrej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502924/
https://www.ncbi.nlm.nih.gov/pubmed/28773383
http://dx.doi.org/10.3390/ma9040260
_version_ 1783249006464335872
author Vesely, Radek
Jelinkova, Pavlina
Hegerova, Dagmar
Cernei, Natalia
Kopel, Pavel
Moulick, Amitava
Richtera, Lukas
Heger, Zbynek
Adam, Vojtech
Zitka, Ondrej
author_facet Vesely, Radek
Jelinkova, Pavlina
Hegerova, Dagmar
Cernei, Natalia
Kopel, Pavel
Moulick, Amitava
Richtera, Lukas
Heger, Zbynek
Adam, Vojtech
Zitka, Ondrej
author_sort Vesely, Radek
collection PubMed
description This paper investigates the synthesis of paramagnetic nanoparticles, which are able to bind branched chain amino acids (BCAAs)—leucine, valine, and isoleucine and, thus, serve as a tool for their isolation. Further, by this, we present an approach for encapsulation of nanoparticles into a liposome cavity resulting in a delivery system. Analyses of valine and leucine in entire complex show that 31.3% and 32.6% recoveries are reached for those amino acids. Evaluation of results shows that the success rate of delivery in Escherichia coli (E. coli) is higher in the case of BCAAs on nanoparticles entrapped in liposomes (28.7% and 34.7% for valine and leucine, respectively) when compared to nanoparticles with no liposomal envelope (18.3% and 13.7% for valine and leucine, respectively). The nanoparticles with no liposomal envelope exhibit the negative zeta potential (−9.1 ± 0.3 mV); however, their encapsulation results in a shift into positive values (range of 28.9 ± 0.4 to 33.1 ± 0.5 mV). Thus, electrostatic interactions with negatively-charged cell membranes (approx. −50 mV in the case of E. coli) leads to a better uptake of cargo. Our delivery system was finally tested with the leucine-rich antimicrobial peptide (FALALKALKKALKKLKKALKKAL) and it is shown that hemocompatibility (7.5%) and antimicrobial activity of the entire complex against E. coli, Staphylococcus aureus (S. aureus), and methicilin-resistant S. aureus (MRSA) is comparable or better than conventional penicillin antibiotics.
format Online
Article
Text
id pubmed-5502924
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-55029242017-07-28 Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides Vesely, Radek Jelinkova, Pavlina Hegerova, Dagmar Cernei, Natalia Kopel, Pavel Moulick, Amitava Richtera, Lukas Heger, Zbynek Adam, Vojtech Zitka, Ondrej Materials (Basel) Article This paper investigates the synthesis of paramagnetic nanoparticles, which are able to bind branched chain amino acids (BCAAs)—leucine, valine, and isoleucine and, thus, serve as a tool for their isolation. Further, by this, we present an approach for encapsulation of nanoparticles into a liposome cavity resulting in a delivery system. Analyses of valine and leucine in entire complex show that 31.3% and 32.6% recoveries are reached for those amino acids. Evaluation of results shows that the success rate of delivery in Escherichia coli (E. coli) is higher in the case of BCAAs on nanoparticles entrapped in liposomes (28.7% and 34.7% for valine and leucine, respectively) when compared to nanoparticles with no liposomal envelope (18.3% and 13.7% for valine and leucine, respectively). The nanoparticles with no liposomal envelope exhibit the negative zeta potential (−9.1 ± 0.3 mV); however, their encapsulation results in a shift into positive values (range of 28.9 ± 0.4 to 33.1 ± 0.5 mV). Thus, electrostatic interactions with negatively-charged cell membranes (approx. −50 mV in the case of E. coli) leads to a better uptake of cargo. Our delivery system was finally tested with the leucine-rich antimicrobial peptide (FALALKALKKALKKLKKALKKAL) and it is shown that hemocompatibility (7.5%) and antimicrobial activity of the entire complex against E. coli, Staphylococcus aureus (S. aureus), and methicilin-resistant S. aureus (MRSA) is comparable or better than conventional penicillin antibiotics. MDPI 2016-03-31 /pmc/articles/PMC5502924/ /pubmed/28773383 http://dx.doi.org/10.3390/ma9040260 Text en © 2016 by the authors; Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vesely, Radek
Jelinkova, Pavlina
Hegerova, Dagmar
Cernei, Natalia
Kopel, Pavel
Moulick, Amitava
Richtera, Lukas
Heger, Zbynek
Adam, Vojtech
Zitka, Ondrej
Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides
title Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides
title_full Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides
title_fullStr Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides
title_full_unstemmed Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides
title_short Nanoparticles Suitable for BCAA Isolation Can Serve for Use in Magnetic Lipoplex-Based Delivery System for L, I, V, or R-rich Antimicrobial Peptides
title_sort nanoparticles suitable for bcaa isolation can serve for use in magnetic lipoplex-based delivery system for l, i, v, or r-rich antimicrobial peptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502924/
https://www.ncbi.nlm.nih.gov/pubmed/28773383
http://dx.doi.org/10.3390/ma9040260
work_keys_str_mv AT veselyradek nanoparticlessuitableforbcaaisolationcanserveforuseinmagneticlipoplexbaseddeliverysystemforlivorrrichantimicrobialpeptides
AT jelinkovapavlina nanoparticlessuitableforbcaaisolationcanserveforuseinmagneticlipoplexbaseddeliverysystemforlivorrrichantimicrobialpeptides
AT hegerovadagmar nanoparticlessuitableforbcaaisolationcanserveforuseinmagneticlipoplexbaseddeliverysystemforlivorrrichantimicrobialpeptides
AT cerneinatalia nanoparticlessuitableforbcaaisolationcanserveforuseinmagneticlipoplexbaseddeliverysystemforlivorrrichantimicrobialpeptides
AT kopelpavel nanoparticlessuitableforbcaaisolationcanserveforuseinmagneticlipoplexbaseddeliverysystemforlivorrrichantimicrobialpeptides
AT moulickamitava nanoparticlessuitableforbcaaisolationcanserveforuseinmagneticlipoplexbaseddeliverysystemforlivorrrichantimicrobialpeptides
AT richteralukas nanoparticlessuitableforbcaaisolationcanserveforuseinmagneticlipoplexbaseddeliverysystemforlivorrrichantimicrobialpeptides
AT hegerzbynek nanoparticlessuitableforbcaaisolationcanserveforuseinmagneticlipoplexbaseddeliverysystemforlivorrrichantimicrobialpeptides
AT adamvojtech nanoparticlessuitableforbcaaisolationcanserveforuseinmagneticlipoplexbaseddeliverysystemforlivorrrichantimicrobialpeptides
AT zitkaondrej nanoparticlessuitableforbcaaisolationcanserveforuseinmagneticlipoplexbaseddeliverysystemforlivorrrichantimicrobialpeptides