Cargando…
A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering
The concept of biphasic or multi-layered compound scaffolds has been explored within numerous studies in the context of cartilage and osteochondral regeneration. To date, no system has been identified that stands out in terms of superior chondrogenesis, osteogenesis or the formation of a zone of cal...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502978/ https://www.ncbi.nlm.nih.gov/pubmed/28773410 http://dx.doi.org/10.3390/ma9040285 |
_version_ | 1783249017105285120 |
---|---|
author | Bartnikowski, Michal Akkineni, Ashwini Rahul Gelinsky, Michael Woodruff, Maria A. Klein, Travis J. |
author_facet | Bartnikowski, Michal Akkineni, Ashwini Rahul Gelinsky, Michael Woodruff, Maria A. Klein, Travis J. |
author_sort | Bartnikowski, Michal |
collection | PubMed |
description | The concept of biphasic or multi-layered compound scaffolds has been explored within numerous studies in the context of cartilage and osteochondral regeneration. To date, no system has been identified that stands out in terms of superior chondrogenesis, osteogenesis or the formation of a zone of calcified cartilage (ZCC). Herein we present a 3D plotted scaffold, comprising an alginate and hydroxyapatite paste, cast within a photocrosslinkable hydrogel made of gelatin methacrylamide (GelMA), or GelMA with hyaluronic acid methacrylate (HAMA). We hypothesized that this combination of 3D plotting and hydrogel crosslinking would form a high fidelity, cell supporting structure that would allow localization of hydroxyapatite to the deepest regions of the structure whilst taking advantage of hydrogel photocrosslinking. We assessed this preliminary design in terms of chondrogenesis in culture with human articular chondrocytes, and verified whether the inclusion of hydroxyapatite in the form presented had any influence on the formation of the ZCC. Whilst the inclusion of HAMA resulted in a better chondrogenic outcome, the effect of HAP was limited. We overall demonstrated that formation of such compound structures is possible, providing a foundation for future work. The development of cohesive biphasic systems is highly relevant for current and future cartilage tissue engineering. |
format | Online Article Text |
id | pubmed-5502978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-55029782017-07-28 A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering Bartnikowski, Michal Akkineni, Ashwini Rahul Gelinsky, Michael Woodruff, Maria A. Klein, Travis J. Materials (Basel) Article The concept of biphasic or multi-layered compound scaffolds has been explored within numerous studies in the context of cartilage and osteochondral regeneration. To date, no system has been identified that stands out in terms of superior chondrogenesis, osteogenesis or the formation of a zone of calcified cartilage (ZCC). Herein we present a 3D plotted scaffold, comprising an alginate and hydroxyapatite paste, cast within a photocrosslinkable hydrogel made of gelatin methacrylamide (GelMA), or GelMA with hyaluronic acid methacrylate (HAMA). We hypothesized that this combination of 3D plotting and hydrogel crosslinking would form a high fidelity, cell supporting structure that would allow localization of hydroxyapatite to the deepest regions of the structure whilst taking advantage of hydrogel photocrosslinking. We assessed this preliminary design in terms of chondrogenesis in culture with human articular chondrocytes, and verified whether the inclusion of hydroxyapatite in the form presented had any influence on the formation of the ZCC. Whilst the inclusion of HAMA resulted in a better chondrogenic outcome, the effect of HAP was limited. We overall demonstrated that formation of such compound structures is possible, providing a foundation for future work. The development of cohesive biphasic systems is highly relevant for current and future cartilage tissue engineering. MDPI 2016-04-14 /pmc/articles/PMC5502978/ /pubmed/28773410 http://dx.doi.org/10.3390/ma9040285 Text en © 2016 by the authors; Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bartnikowski, Michal Akkineni, Ashwini Rahul Gelinsky, Michael Woodruff, Maria A. Klein, Travis J. A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering |
title | A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering |
title_full | A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering |
title_fullStr | A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering |
title_full_unstemmed | A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering |
title_short | A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering |
title_sort | hydrogel model incorporating 3d-plotted hydroxyapatite for osteochondral tissue engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502978/ https://www.ncbi.nlm.nih.gov/pubmed/28773410 http://dx.doi.org/10.3390/ma9040285 |
work_keys_str_mv | AT bartnikowskimichal ahydrogelmodelincorporating3dplottedhydroxyapatiteforosteochondraltissueengineering AT akkineniashwinirahul ahydrogelmodelincorporating3dplottedhydroxyapatiteforosteochondraltissueengineering AT gelinskymichael ahydrogelmodelincorporating3dplottedhydroxyapatiteforosteochondraltissueengineering AT woodruffmariaa ahydrogelmodelincorporating3dplottedhydroxyapatiteforosteochondraltissueengineering AT kleintravisj ahydrogelmodelincorporating3dplottedhydroxyapatiteforosteochondraltissueengineering AT bartnikowskimichal hydrogelmodelincorporating3dplottedhydroxyapatiteforosteochondraltissueengineering AT akkineniashwinirahul hydrogelmodelincorporating3dplottedhydroxyapatiteforosteochondraltissueengineering AT gelinskymichael hydrogelmodelincorporating3dplottedhydroxyapatiteforosteochondraltissueengineering AT woodruffmariaa hydrogelmodelincorporating3dplottedhydroxyapatiteforosteochondraltissueengineering AT kleintravisj hydrogelmodelincorporating3dplottedhydroxyapatiteforosteochondraltissueengineering |