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Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells

Within nanotechnology, gold and silver nanostructures have unique physical, chemical, and electronic properties [1,2], which make them suitable for a number of applications. Moreover, biosynthetic methods are considered to be a safer alternative to conventional physicochemical procedures for both th...

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Autores principales: Gorbe, Mónica, Bhat, Ravishankar, Aznar, Elena, Sancenón, Félix, Marcos, M. Dolores, Herraiz, F. Javier, Prohens, Jaime, Venkataraman, Abbaraju, Martínez-Máñez, Ramón
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503040/
https://www.ncbi.nlm.nih.gov/pubmed/28773448
http://dx.doi.org/10.3390/ma9050325
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author Gorbe, Mónica
Bhat, Ravishankar
Aznar, Elena
Sancenón, Félix
Marcos, M. Dolores
Herraiz, F. Javier
Prohens, Jaime
Venkataraman, Abbaraju
Martínez-Máñez, Ramón
author_facet Gorbe, Mónica
Bhat, Ravishankar
Aznar, Elena
Sancenón, Félix
Marcos, M. Dolores
Herraiz, F. Javier
Prohens, Jaime
Venkataraman, Abbaraju
Martínez-Máñez, Ramón
author_sort Gorbe, Mónica
collection PubMed
description Within nanotechnology, gold and silver nanostructures have unique physical, chemical, and electronic properties [1,2], which make them suitable for a number of applications. Moreover, biosynthetic methods are considered to be a safer alternative to conventional physicochemical procedures for both the environmental and biomedical applications, due to their eco-friendly nature and the avoidance of toxic chemicals in the synthesis. For this reason, employing bio routes in the synthesis of functionalized silver nanoparticles (FAgNP) have gained importance recently in this field. In the present study, we report the rapid synthesis of FAgNP through the extract of pepino (Solanum muricatum) leaves and employing microwave oven irradiation. The core-shell globular morphology and characterization of the different shaped and sized FAgNP, with a core of 20–50 nm of diameter is established using the UV-Visible spectroscopy (UV-vis), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and Zeta potential and dynamic light scanning (DLS) studies. Moreover, cytotoxic studies employing HeLa (human cervix carcinoma) cells were undertaken to understand FAgNP interactions with cells. HeLa cells showed significant dose dependent antiproliferative activity in the presence of FAgNP at relatively low concentrations. The calculated IC(50) value was 37.5 µg/mL, similar to others obtained for FAgNPs against HeLa cells.
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spelling pubmed-55030402017-07-28 Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells Gorbe, Mónica Bhat, Ravishankar Aznar, Elena Sancenón, Félix Marcos, M. Dolores Herraiz, F. Javier Prohens, Jaime Venkataraman, Abbaraju Martínez-Máñez, Ramón Materials (Basel) Article Within nanotechnology, gold and silver nanostructures have unique physical, chemical, and electronic properties [1,2], which make them suitable for a number of applications. Moreover, biosynthetic methods are considered to be a safer alternative to conventional physicochemical procedures for both the environmental and biomedical applications, due to their eco-friendly nature and the avoidance of toxic chemicals in the synthesis. For this reason, employing bio routes in the synthesis of functionalized silver nanoparticles (FAgNP) have gained importance recently in this field. In the present study, we report the rapid synthesis of FAgNP through the extract of pepino (Solanum muricatum) leaves and employing microwave oven irradiation. The core-shell globular morphology and characterization of the different shaped and sized FAgNP, with a core of 20–50 nm of diameter is established using the UV-Visible spectroscopy (UV-vis), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and Zeta potential and dynamic light scanning (DLS) studies. Moreover, cytotoxic studies employing HeLa (human cervix carcinoma) cells were undertaken to understand FAgNP interactions with cells. HeLa cells showed significant dose dependent antiproliferative activity in the presence of FAgNP at relatively low concentrations. The calculated IC(50) value was 37.5 µg/mL, similar to others obtained for FAgNPs against HeLa cells. MDPI 2016-04-28 /pmc/articles/PMC5503040/ /pubmed/28773448 http://dx.doi.org/10.3390/ma9050325 Text en © 2016 by the authors; Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gorbe, Mónica
Bhat, Ravishankar
Aznar, Elena
Sancenón, Félix
Marcos, M. Dolores
Herraiz, F. Javier
Prohens, Jaime
Venkataraman, Abbaraju
Martínez-Máñez, Ramón
Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells
title Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells
title_full Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells
title_fullStr Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells
title_full_unstemmed Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells
title_short Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells
title_sort rapid biosynthesis of silver nanoparticles using pepino (solanum muricatum) leaf extract and their cytotoxicity on hela cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503040/
https://www.ncbi.nlm.nih.gov/pubmed/28773448
http://dx.doi.org/10.3390/ma9050325
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