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Expression, purification, and contaminant detection for structural studies of Ralstonia metallidurance ClC protein rm1
Single-particle electron cryo-microscopy (cryo-EM) has become a popular method for high-resolution study of the structural and functional properties of proteins. However, sufficient expression and purification of membrane proteins holds many challenges. We describe methods to overcome these obstacle...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503242/ https://www.ncbi.nlm.nih.gov/pubmed/28692650 http://dx.doi.org/10.1371/journal.pone.0180163 |