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WISP-3 inhibition of miR-452 promotes VEGF-A expression in chondrosarcoma cells and induces endothelial progenitor cells angiogenesis

Chondrosarcoma is the second most prevalent general primary tumor of bone following osteosarcoma. Chondrosarcoma development may be linked to angiogenesis, which is principally elicited by vascular endothelial growth factor-A (VEGF-A). VEGF-A level has been recognized as a prognostic marker in angio...

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Autores principales: Lin, Chih-Yang, Tzeng, Huey-En, Li, Te-Mao, Chen, Hsien-Te, Lee, Yi, Yang, Yi-Chen, Wang, Shih-Wei, Yang, Wei-Hung, Tang, Chih-Hsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503633/
https://www.ncbi.nlm.nih.gov/pubmed/28465477
http://dx.doi.org/10.18632/oncotarget.17142
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author Lin, Chih-Yang
Tzeng, Huey-En
Li, Te-Mao
Chen, Hsien-Te
Lee, Yi
Yang, Yi-Chen
Wang, Shih-Wei
Yang, Wei-Hung
Tang, Chih-Hsin
author_facet Lin, Chih-Yang
Tzeng, Huey-En
Li, Te-Mao
Chen, Hsien-Te
Lee, Yi
Yang, Yi-Chen
Wang, Shih-Wei
Yang, Wei-Hung
Tang, Chih-Hsin
author_sort Lin, Chih-Yang
collection PubMed
description Chondrosarcoma is the second most prevalent general primary tumor of bone following osteosarcoma. Chondrosarcoma development may be linked to angiogenesis, which is principally elicited by vascular endothelial growth factor-A (VEGF-A). VEGF-A level has been recognized as a prognostic marker in angiogenesis. WNT1-inducible signaling pathway protein-3 (WISP)-3/CCN6 belongs to the CCN family and is involved in regulating several cellular functions, including cell proliferation, differentiation, and migration. Nevertheless, the effect of WISP-3 on VEGF-A production and angiogenesis in human chondrosarcoma remains largely unknown. This current study shows that WISP-3 promoted VEGF-A production and induced angiogenesis of human endothelial progenitor cells. Moreover, WISP-3-enhanced VEGF-A expression and angiogenesis involved the c-Src and p38 signaling pathways, while miR-452 expression was negatively affected by WISP-3 via the c-Src and p38 pathways. Our results illustrate the clinical significance of WISP-3, VEGF-A and miR-452 in human chondrosarcoma patients. WISP-3 may illustrate a novel therapeutic target in the metastasis and angiogenesis of chondrosarcoma.
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spelling pubmed-55036332017-07-11 WISP-3 inhibition of miR-452 promotes VEGF-A expression in chondrosarcoma cells and induces endothelial progenitor cells angiogenesis Lin, Chih-Yang Tzeng, Huey-En Li, Te-Mao Chen, Hsien-Te Lee, Yi Yang, Yi-Chen Wang, Shih-Wei Yang, Wei-Hung Tang, Chih-Hsin Oncotarget Research Paper Chondrosarcoma is the second most prevalent general primary tumor of bone following osteosarcoma. Chondrosarcoma development may be linked to angiogenesis, which is principally elicited by vascular endothelial growth factor-A (VEGF-A). VEGF-A level has been recognized as a prognostic marker in angiogenesis. WNT1-inducible signaling pathway protein-3 (WISP)-3/CCN6 belongs to the CCN family and is involved in regulating several cellular functions, including cell proliferation, differentiation, and migration. Nevertheless, the effect of WISP-3 on VEGF-A production and angiogenesis in human chondrosarcoma remains largely unknown. This current study shows that WISP-3 promoted VEGF-A production and induced angiogenesis of human endothelial progenitor cells. Moreover, WISP-3-enhanced VEGF-A expression and angiogenesis involved the c-Src and p38 signaling pathways, while miR-452 expression was negatively affected by WISP-3 via the c-Src and p38 pathways. Our results illustrate the clinical significance of WISP-3, VEGF-A and miR-452 in human chondrosarcoma patients. WISP-3 may illustrate a novel therapeutic target in the metastasis and angiogenesis of chondrosarcoma. Impact Journals LLC 2017-04-17 /pmc/articles/PMC5503633/ /pubmed/28465477 http://dx.doi.org/10.18632/oncotarget.17142 Text en Copyright: © 2017 Lin et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Lin, Chih-Yang
Tzeng, Huey-En
Li, Te-Mao
Chen, Hsien-Te
Lee, Yi
Yang, Yi-Chen
Wang, Shih-Wei
Yang, Wei-Hung
Tang, Chih-Hsin
WISP-3 inhibition of miR-452 promotes VEGF-A expression in chondrosarcoma cells and induces endothelial progenitor cells angiogenesis
title WISP-3 inhibition of miR-452 promotes VEGF-A expression in chondrosarcoma cells and induces endothelial progenitor cells angiogenesis
title_full WISP-3 inhibition of miR-452 promotes VEGF-A expression in chondrosarcoma cells and induces endothelial progenitor cells angiogenesis
title_fullStr WISP-3 inhibition of miR-452 promotes VEGF-A expression in chondrosarcoma cells and induces endothelial progenitor cells angiogenesis
title_full_unstemmed WISP-3 inhibition of miR-452 promotes VEGF-A expression in chondrosarcoma cells and induces endothelial progenitor cells angiogenesis
title_short WISP-3 inhibition of miR-452 promotes VEGF-A expression in chondrosarcoma cells and induces endothelial progenitor cells angiogenesis
title_sort wisp-3 inhibition of mir-452 promotes vegf-a expression in chondrosarcoma cells and induces endothelial progenitor cells angiogenesis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503633/
https://www.ncbi.nlm.nih.gov/pubmed/28465477
http://dx.doi.org/10.18632/oncotarget.17142
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