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Transcriptomic profiling of hemp bast fibres at different developmental stages
Bast fibres are long extraxylary cells which mechanically support the phloem and they are divided into xylan- and gelatinous-type, depending on the composition of their secondary cell walls. The former, typical of jute/kenaf bast fibres, are characterized by the presence of xylan and a high degree o...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5504027/ https://www.ncbi.nlm.nih.gov/pubmed/28694530 http://dx.doi.org/10.1038/s41598-017-05200-8 |
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author | Guerriero, Gea Behr, Marc Legay, Sylvain Mangeot-Peter, Lauralie Zorzan, Simone Ghoniem, Mohammad Hausman, Jean-Francois |
author_facet | Guerriero, Gea Behr, Marc Legay, Sylvain Mangeot-Peter, Lauralie Zorzan, Simone Ghoniem, Mohammad Hausman, Jean-Francois |
author_sort | Guerriero, Gea |
collection | PubMed |
description | Bast fibres are long extraxylary cells which mechanically support the phloem and they are divided into xylan- and gelatinous-type, depending on the composition of their secondary cell walls. The former, typical of jute/kenaf bast fibres, are characterized by the presence of xylan and a high degree of lignification, while the latter, found in tension wood, as well as flax, ramie and hemp bast fibres, have a high abundance of crystalline cellulose. During their differentiation, bast fibres undergo specific developmental stages: the cells initially elongate rapidly by intrusive growth, subsequently they cease elongation and start to thicken. The goal of the present study is to provide a transcriptomic close-up of the key events accompanying bast fibre development in textile hemp (Cannabis sativa L.), a fibre crop of great importance. Bast fibres have been sampled from different stem regions. The developmental stages corresponding to active elongation and cell wall thickening have been studied using RNA-Seq. The results show that the fibres sampled at each stem region are characterized by a specific transcriptomic signature and that the major changes in cell wall-related processes take place at the internode containing the snap point. The data generated also identify several interesting candidates for future functional analysis. |
format | Online Article Text |
id | pubmed-5504027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55040272017-07-12 Transcriptomic profiling of hemp bast fibres at different developmental stages Guerriero, Gea Behr, Marc Legay, Sylvain Mangeot-Peter, Lauralie Zorzan, Simone Ghoniem, Mohammad Hausman, Jean-Francois Sci Rep Article Bast fibres are long extraxylary cells which mechanically support the phloem and they are divided into xylan- and gelatinous-type, depending on the composition of their secondary cell walls. The former, typical of jute/kenaf bast fibres, are characterized by the presence of xylan and a high degree of lignification, while the latter, found in tension wood, as well as flax, ramie and hemp bast fibres, have a high abundance of crystalline cellulose. During their differentiation, bast fibres undergo specific developmental stages: the cells initially elongate rapidly by intrusive growth, subsequently they cease elongation and start to thicken. The goal of the present study is to provide a transcriptomic close-up of the key events accompanying bast fibre development in textile hemp (Cannabis sativa L.), a fibre crop of great importance. Bast fibres have been sampled from different stem regions. The developmental stages corresponding to active elongation and cell wall thickening have been studied using RNA-Seq. The results show that the fibres sampled at each stem region are characterized by a specific transcriptomic signature and that the major changes in cell wall-related processes take place at the internode containing the snap point. The data generated also identify several interesting candidates for future functional analysis. Nature Publishing Group UK 2017-07-10 /pmc/articles/PMC5504027/ /pubmed/28694530 http://dx.doi.org/10.1038/s41598-017-05200-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Guerriero, Gea Behr, Marc Legay, Sylvain Mangeot-Peter, Lauralie Zorzan, Simone Ghoniem, Mohammad Hausman, Jean-Francois Transcriptomic profiling of hemp bast fibres at different developmental stages |
title | Transcriptomic profiling of hemp bast fibres at different developmental stages |
title_full | Transcriptomic profiling of hemp bast fibres at different developmental stages |
title_fullStr | Transcriptomic profiling of hemp bast fibres at different developmental stages |
title_full_unstemmed | Transcriptomic profiling of hemp bast fibres at different developmental stages |
title_short | Transcriptomic profiling of hemp bast fibres at different developmental stages |
title_sort | transcriptomic profiling of hemp bast fibres at different developmental stages |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5504027/ https://www.ncbi.nlm.nih.gov/pubmed/28694530 http://dx.doi.org/10.1038/s41598-017-05200-8 |
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